To assess the adequacy of oxygen availability and utilization within the cerebral cortex in vivo, we have measured the partial pressure of oxygen in tissue (PtO2), as well as the reduction oxidation state of cytochrome c oxidase (cyt aa3) during shock induced by slow or rapid hemorrhage in anesthetized cats. PtO2 was measured with pyrenebutyric acid-generated fluorescence in cerebral cortical cells. Cyt aa3 redox state was measured by the absorption of monochromatic light at 605 nm absorption peak of the enzyme reflected from the same cortical field. The PtO2 remained within the normal range until either 30 +/- 1.5 ml blood/kg was removed or the mean arterial pressure fell by 70 +/- 5% of base line. Beyond either point, the PtO2 fell rapidly to a low value approximating zero. By contrast, the reduction of cyt aa3 began early when as little as 5 ml blood/kg was removed. Thereafter, the shift toward reduction was progressive and continuous with a slow rate at first and a rapid rate later. This accelerated rate of cyt aa3 reduction preceded the rapid fall of PtO2. We concluded that, under these experimental conditions, cyt aa3 reduction is a much earlier and more sensitive indicator of perturbed intracellular aerobic metabolism due to hemorrhage that is PtO2.
K Kariman, F G Hempel, F F Jöbsis, S R Burns, H A Saltzman
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Monitoring Tissue Perfusion, Oxygenation, and Metabolism in Critically Ill Patients
NJ Ekbal, A Dyson, C Black, M Singer |
Chest | 2013 |
Cytochrome Spectroscopy in Scattering Suspensions Containing Mitochondria and Red Blood Cells
EL Hull, TH Foster |
Applied Spectroscopy | 2001 |
Escherichia coli endotoxin reduces cytochrome aa3 redox status in pig skeletal muscle:
AP Forget, J Mangalaboyi, S Mordon, B Guery, B Vallet, F Fourrier, C Chopin |
Critical Care Medicine | 2000 |
Redox status of cytochrome a,a3: A noninvasive indicator of dysoxia in regional hypoxic or ischemic hypoxia
BP Guery, J Mangalaboyi, P Menager, S Mordon, B Vallet, C Chopin |
Critical Care Medicine | 1999 |
Significance of elevated cytochrome aa3 in a state of endotoxemia in dogs
H Ogata, M Mishio, XX Luo, X Xu |
Resuscitation | 1996 |
Neurochemical Monitoring in the Intensive Care Unit
T Tsubokawa, A Marmarou, C Robertson, G Teasdale |
1995 | |
Physiological Aspects of Biosynthesis of Lignin Peroxidases by Phanerochaete chrysosporium
D Lestan, M Lestan, A Perdih |
Applied and environmental microbiology | 1994 |
Analysis of Fungal Pellets by UV-Visible Spectrum Diffuse Reflectance Spectroscopy
D Lestan, H Podgornik, A Perdih |
Applied and environmental microbiology | 1993 |
Mitochondrial Dysfunction
T Inoue, K Tagawa |
Mitochondrial Dysfunction | 1993 |
Cerebral oxygen monitoring with near infrared spectroscopy: Clinical application to neonates
JE Brazy |
Journal of Clinical Monitoring | 1991 |
Uncoupling effects of 2,4-dinitrophenol on electron transfer reactions and cell bioenergetics in rat brain in situ
K Kariman, B Chance, DS Burkhart, LA Bolinger |
Brain Research | 1986 |
Non-invasive in vivo spectrophotometric monitoring of brain cytochrome aa3 revisited
K Kariman, DS Burkhart |
Brain Research | 1985 |
Heme-copper relationship of cytochrome oxidase in rat brain
K Kariman, DS Burkhart |
Biochemical and Biophysical Research Communications | 1985 |
Evidence for Adenosine Triphosphate Degradation in Critically-Ill Patients
CM Grum, RH Simon, DR Dantzker, IH Fox |
Chest | 1985 |
Cytochrome a,a3 reoxidation. Early indicator of metabolic recovery from hemorrhagic shock in rats
K Kariman, FF Jöbsis, HA Saltzman |
Journal of Clinical Investigation | 1983 |
Effect of Acute Arterial Hypo- and Hypertension on Cerebrocortical NAD/NADH Redox State and Vascular Volume
E Dóra, AG Kovách |
Journal of Cerebral Blood Flow & Metabolism | 1982 |