Complement-independent Adherence of Escherichia coli to Complement Receptors In Vitro

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Abstract

We studied adherence to human cells by a strain of Escherichia coli. Adherence to erythrocytes was assessed directly by phase-contrast microscopy and indirectly by hemagglutination; adherence to peripheral blood leukocytes, using radiolabeled bacteria and subsequent determination of leukocyte-associated radioactivity; and adherence to renal glomeruli, by microscopy of fluoresceinated bacteria and of Gram-stained nonfluoresceinated bacteria.

Authors

Douglas P. Fine, Barbara L. Harper, Edward D. Carpenter, C. Patrick Davis, Tito Cavallo, James C. Guckian