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Research Article Free access | 10.1172/JCI109807
Department of Medicine, Granulocyte Transfusion Service, University of Iowa, Iowa City, Iowa
Department of Pediatrics, Granulocyte Transfusion Service, University of Iowa, Iowa City, Iowa
Department of Pathology, Granulocyte Transfusion Service, University of Iowa, Iowa City, Iowa
Veterans Administration Medical Center, Iowa City, Iowa
Department of Immunohaematology, University Hospital, Leiden, Netherlands
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Department of Medicine, Granulocyte Transfusion Service, University of Iowa, Iowa City, Iowa
Department of Pediatrics, Granulocyte Transfusion Service, University of Iowa, Iowa City, Iowa
Department of Pathology, Granulocyte Transfusion Service, University of Iowa, Iowa City, Iowa
Veterans Administration Medical Center, Iowa City, Iowa
Department of Immunohaematology, University Hospital, Leiden, Netherlands
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Department of Medicine, Granulocyte Transfusion Service, University of Iowa, Iowa City, Iowa
Department of Pediatrics, Granulocyte Transfusion Service, University of Iowa, Iowa City, Iowa
Department of Pathology, Granulocyte Transfusion Service, University of Iowa, Iowa City, Iowa
Veterans Administration Medical Center, Iowa City, Iowa
Department of Immunohaematology, University Hospital, Leiden, Netherlands
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Department of Medicine, Granulocyte Transfusion Service, University of Iowa, Iowa City, Iowa
Department of Pediatrics, Granulocyte Transfusion Service, University of Iowa, Iowa City, Iowa
Department of Pathology, Granulocyte Transfusion Service, University of Iowa, Iowa City, Iowa
Veterans Administration Medical Center, Iowa City, Iowa
Department of Immunohaematology, University Hospital, Leiden, Netherlands
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Department of Medicine, Granulocyte Transfusion Service, University of Iowa, Iowa City, Iowa
Department of Pediatrics, Granulocyte Transfusion Service, University of Iowa, Iowa City, Iowa
Department of Pathology, Granulocyte Transfusion Service, University of Iowa, Iowa City, Iowa
Veterans Administration Medical Center, Iowa City, Iowa
Department of Immunohaematology, University Hospital, Leiden, Netherlands
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Department of Medicine, Granulocyte Transfusion Service, University of Iowa, Iowa City, Iowa
Department of Pediatrics, Granulocyte Transfusion Service, University of Iowa, Iowa City, Iowa
Department of Pathology, Granulocyte Transfusion Service, University of Iowa, Iowa City, Iowa
Veterans Administration Medical Center, Iowa City, Iowa
Department of Immunohaematology, University Hospital, Leiden, Netherlands
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Department of Medicine, Granulocyte Transfusion Service, University of Iowa, Iowa City, Iowa
Department of Pediatrics, Granulocyte Transfusion Service, University of Iowa, Iowa City, Iowa
Department of Pathology, Granulocyte Transfusion Service, University of Iowa, Iowa City, Iowa
Veterans Administration Medical Center, Iowa City, Iowa
Department of Immunohaematology, University Hospital, Leiden, Netherlands
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Department of Medicine, Granulocyte Transfusion Service, University of Iowa, Iowa City, Iowa
Department of Pediatrics, Granulocyte Transfusion Service, University of Iowa, Iowa City, Iowa
Department of Pathology, Granulocyte Transfusion Service, University of Iowa, Iowa City, Iowa
Veterans Administration Medical Center, Iowa City, Iowa
Department of Immunohaematology, University Hospital, Leiden, Netherlands
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Department of Medicine, Granulocyte Transfusion Service, University of Iowa, Iowa City, Iowa
Department of Pediatrics, Granulocyte Transfusion Service, University of Iowa, Iowa City, Iowa
Department of Pathology, Granulocyte Transfusion Service, University of Iowa, Iowa City, Iowa
Veterans Administration Medical Center, Iowa City, Iowa
Department of Immunohaematology, University Hospital, Leiden, Netherlands
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Published June 1, 1980 - More info
Although granulocyte transfusions and bone marrow transplantation are becoming common clinical modalities, our knowledge of surface nonerythroid, nonlymphoid, non-HLA hematopoetic antigens remains very incomplete. Accordingly, we have systematically screened sera from recipients of multiple granulocyte and whole blood transfusions, and immunoneutropenic patients for antibodies directed primarily at granulocytes.
The initial screens demonstrated that >50% of the sera from the above sources contained non-HLA cytotoxic and/or agglutinating antibodies. Preliminary clustering indicated seven possible new specificities detected by microgranulocytotoxicity. Calculations for Hardy-Weinberg goodness of fit based on a study of 98 unrelated donors plus informative families established that 5 of these were alleles of a single new locus termed Human Granulocyte Antigen (HGA)-3a, b, c, d, and e. Absorptions indicated that these antigens were present on mature granulocytes but absent from platelets, lymphocytes, monocytes, and myeloid precursors. A single antigen of another separate locus, HGA-1, was also identified. Absorptions revealed a quite different distribution for HGA-1 than HGA-3, this antigen being detected on monocytes and myeloblasts as well as on mature granulocytes. Independent segregation of the three loci from HLA, from the NA-NB and the 5a-5b antigens, and from themselves was confirmed in informative families.
Finally, it seems likely that other antigens will be identified because several other sera that react with both monocytes and granulocytes have been detected.