Past investigation has revealed that the circadian rhythm of intestinal sucrase activity in rats is primarily cued by the time of feeding. We examined the mechanism of the circadian rhythm by methods involving quantitative immunoprecipitation of sucrase-isomaltase protein and study of decay of radioactively labeled protein. Rats were placed on a controlled feeding regimen (1000-1500 h) and then sacrificed at 3-h intervals over a 24-h period. Immunotitration experiments indicated that the circadian rhythm was the result of changes in the absolute amount of sucrase-isomaltase protein present and not of changes in the enzyme's catalytic efficiency.
Mark A. Kaufman, Helen A. Korsmo, Ward A. Olsen
Title and authors | Publication | Year |
---|---|---|
Gastrointestinal toxicity of mycophenolate mofetil in rats: Effect of administration time
I Dridi, W Ben-Cherif, Z Haouas, K Aouam, M Ben-Attia, A Reinberg, NA Boughattas |
Chronobiology International | 2015 |
Clock genes regulate the feeding schedule-dependent diurnal rhythm changes in hexose transporter gene expressions through the binding of BMAL1 to the promoter/enhancer and transcribed regions
I Iwashina, K Mochizuki, Y Inamochi, T Goda |
The Journal of Nutritional Biochemistry | 2011 |
Coordinated regulation of circadian rhythms and homeostasis by the suprachiasmatic nucleus
H NAKAGAWA, N OKUMURA |
Proceedings of the Japan Academy, Series B | 2010 |
Diurnal rhythmicity in intestinal SGLT-1 function, Vmax , and mRNA expression topography
A Tavakkolizadeh, UV Berger, KR Shen, LL Levitsky, MJ Zinner, MA Hediger, SW Ashley, EE Whang, DB Rhoads |
AJP Gastrointestinal and Liver Physiology | 2001 |
Biological clocks and the digestive system
LA Scheving |
Gastroenterology | 2000 |
Determination of protein synthesis in human ileum in situ by continuous [1- 13 C]leucine infusion
P Rittler, H Demmelmair, B Koletzko, FW Schildberg, WH Hartl |
American journal of physiology. Endocrinology and metabolism | 2000 |
Circadian regulation of uroguanylin and guanylin in the rat intestine
LA Scheving, WH Jin |
American journal of physiology. Cell physiology | 1999 |
Dietary-induced increases of disaccharidase activities in rat jejunum
BK Santos, T Goda, O Koldovsky |
British Journal of Nutrition | 1992 |
Molecular cloning and characterization of a rat intestinal sucrase-isomaltase cDNA. Regulation of sucrase-isomaltase gene expression by sucrose feeding
JP Broyart, JP Hugot, C Perret, A Porteu |
Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression | 1990 |
Quantitative aspects of circadian rhythm in peptide hydrolases and disaccharidases from rat intestinal brush border in vivo
R Ungerer, WT Hekkens |
Journal of Interdisiplinary Cycle Research | 1989 |
Posttranslational Regulation of Sucrase-Isomaltase Expression in Intestinal Crypt and Villus Cells
JF Beaulieu, B Nichols, A Quaroni |
The Journal of biological chemistry | 1989 |
Immunological Aspects
JR Harris |
1988 | |
A Study of the Molecular Pathology of Sucrase-Isomaltase Deficiency
ML Lloyd, WA Olsen |
New England Journal of Medicine | 1987 |
Localization of sucrase-isomaltase in the rat enterocyte
V Lorenzsonn, H Korsmo, WA Olsen |
Gastroenterology | 1987 |
Diarrhoea Associated with Carbohydrate Malabsorption
WF Caspary |
1986 | |
Influence of a Dorsomedial Hypothalamus Lesion on the Circadian Changes in the Enzyme Development and Activity of Intestinal Brushborder Membranes in the Rat
WT Hekkens, PA van Rijn, J Lukač-bajalo, R Ungerer, WJ Rietveld |
Chronobiology International | 1985 |
Circadian rhythm of the pancreatic enzymes in rats: Its relation to small intestinal disaccharidase
DE George, E Lebenthal, M Landis |
Nutrition research (New York, N.Y.) | 1985 |
Advances in Radiation Biology
SK Calderwood, JA Dickson |
Advances in Radiation Biology | 1983 |
Sucrase-Isomaltase Regulation by Dietary Sucrose in the Rat
JP Cézard, JP Broyart, P Cuisinier-Gleizes, H Mathieu |
Gastroenterology | 1983 |
Carbohydrate Absorption and Malabsorption
WJ RAVICH, TM Bayless |
1983 | |
The effect of feeding and fasting on the activity of acyl-CoA:cholesterol acyltransferase in rat small intestine
P Helgerud, R Haugen, KR Norum |
European Journal of Clinical Investigation | 1982 |
Effects of substitution of glucose-oligosaccharides by sucrose in a defined formula diet on intestinal disaccharidases, hepatic lipogenic enzymes and carbohydrate metabolism in young rats
CJ Cha, HT Randall |
Metabolism | 1982 |