Jejunal perfusion studies were performed in 16 healthy volunteers to test the hypothesis that intraluminal cholesterol can mitigate the fluid secretion induced by dihydroxy bile acids and fatty acids. Fluid secretion in the presence of 5 mM taurodeoxycholate was somewhat reduced by 4 mM mono-olein which was used for the solubilization of cholesterol. Addition of 0.8 mM cholesterol reduced fluid secretion further (P less than 0.05). Fluid secretion induced by 4 mM oleic acid was changed to net absorption in a linear fashion with increasing cholesterol concentration in the perfusion solutions. 1 mM cholesterol reduced fluid secretion induced by 6 mM oleic acid (P less than 0.005), but had no effect on fluid secretion induced by 6 mM linolenic acid. Glucose absorption was generally affected in a similar manner as water transport. In vitro, 1 mM cholesterol reduced monomer activity of 6 mM oleic acid to 72.3 +/- 0.9% of control and that of linolenic acid to 81.1 +/- 1.7% of control. Although statistically significant (P less than 0.001), the difference in the effects of cholesterol on monomer activities of the two fatty acids was rather small and it is unlikely that changes in monomer concentration of fatty acids and bile acids account for the protective effect of cholesterol. The in vivo observations point to a new physiological role for biliary cholesterol: the modification of the response of the small intestine to the effects of dihydroxy bile acids and fatty acids.
S L Broor, T Slota, H V Ammon
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Dualistic acidic and neutral glucose fermentation balance in small intestine: Simulation in vitro
E Hakalehto, T Humppi, H Paakkanen |
Pathophysiology | 2008 |
Hepatic cholestasis during the post-embryonic development of fish larvae
JP Diaz, L Mani-Ponset, E Guyot, R Connes |
The Journal of Experimental Zoology | 1998 |
Protective role of biliary cholesterol and phospholipid lamellae against bile acid-induced cell damage
L Puglielli, L Amigo, M Arrese, L Núñez, A Rigotti, J Garrido, S González, G Mingrone, AV Greco, L Accatino, F Nervi |
Gastroenterology | 1994 |
Protection by Dietary Proteins Against the Effects of Bile Acids on Rat Jejunum and Stomach
JF Kruidenier, HV Ammon, UK Charaf, LG Walter |
Gastroenterology | 1985 |
Hepatic Free Fatty Acids in Alcoholic Liver Disease and Morbid Obesity
PG Mavrelis, HV Ammon, JJ Gleysteen, RA Komorowski, UK Charaf |
Hepatology | 1983 |
Effects of lysophosphatidylcholine on jejunal water and solute transport in the rat in vivo
HV Ammon, RE Loeffler, LA Luedtke |
Lipids | 1983 |
Comparison of cholesterol and beta-sitosterol: effects on jejunal fluid secretion induced by oleate, and absorption from mixed micellar solutions.
Slota T, Kozlov NA, Ammon HV |
Gut | 1983 |
Bile Acid-Dependent Secretion of Alkaline Phosphatase in Rat Bile
DE Hatoff, WG Hardison |
Hepatology | 1982 |
Regulation of 3-hydroxy-3-methylglutaryl CoA reductase by analogs of cholesterol and bile acids in cultured intestinal mucosa
EF Stange, A Schneider, G Preclik, M Alavi, H Ditschuneit |
Lipids | 1981 |
Bile acid induced interconversion of 3-hydroxy-3-methylglutaryl coenzyme a reductase in cultured intestine
EF Stange, A Schneider, G Preclik, H Ditschuneit |
Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism | 1981 |
Effect of glucose on jejunal water and solute absorption in the presence of glycodeoxycholate and oleate in man.
Brown BD, Ammon HV |
Digestive Diseases and Sciences | 1981 |