Exposure of rats to high oxygen tensions causes increased collagen content of lungs and alveolar enlargement in 3-6 wk. We tested whether cis-hydroxyproline, a proline analogue that inhibits collagen synthesis, could prevent the collagen accumulation and alveolar enlargement. Rats were exposed to hyperoxia for 60 h and then to room air and hyperoxia for alternate 24-h periods for 11.5 d. Treated oxygen-exposed rats received 200 mg/kg cis-hydroxyproline twice daily over the 14-d exposure period. Control rats breathed room air. Examination of lungs on day 14 showed collagen content of oxygen-exposed lungs to be 48% greater than control (P < 0.05). The collagen content of the treated oxygen-exposed lungs was −12% of control (NS). Total lung volume was 16% greater than control in oxygen-exposed rats (P < 0.05) and 8% greater than control in treated oxygen-exposed rats (NS). Morphometric studies showed alveolar size was greater than control in oxygen-exposed rats (188±11 [SE] vs. 143±6 μμl [P < 0.05]). Oxygen-exposed, treated rats had a mean alveolar volume of 150±7 μμl. Lung pressure-volume curves were significantly shifted to the left of control in the oxygen-exposed rats, whereas the curves of the oxygen-exposed, treated group were identical to control. These data suggest that cis-hydroxyproline prevented the accumulation of collagen in the lungs in pulmonary oxygen toxicity. In addition, there was apparent protection from airspace dilatation and decreased lung elasticity, suggesting that alveolar enlargement after oxygen toxicity is linked to the deposition in lung tissue of new connective tissue fibers.
David J. Riley, Richard A. Berg, Norman H. Edelman, Darwin J. Prockop
Title and authors | Publication | Year |
---|---|---|
N-Vanillylnonanamide, a natural product from capsicum oleoresin, as potential inhibitor of collagen fibrillation
M Visalatchi, B Madhan, G Shanmugam |
International Journal of Biological Macromolecules | 2019 |
Collagen VI deficiency reduces muscle pathology, but does not improve muscle function, in the γ-sarcoglycan-null mouse
JC de Greef, R Hamlyn, BS Jensen, RO Landa, JR Levy, K Kobuke, KP Campbell |
Human Molecular Genetics | 2016 |
Collagen Inhibitory Peptide R1R2 Mediates Vascular Remodeling by Decreasing Inflammation and Smooth Muscle Cell Activation
TH Lee, J Sottile, HY Chiang, AP Gadeau |
PloS one | 2015 |
Properties, metabolisms, and applications of l-proline analogues
TM Bach, H Takagi |
Applied Microbiology and Biotechnology | 2013 |
Production of N-acetyl cis-4-hydroxy-l-proline by the yeast N-acetyltransferase Mpr1
BT Hoa, T Hibi, R Nasuno, G Matsuo, Y Sasano, H Takagi |
Journal of Bioscience and Bioengineering | 2012 |
Microbial production of N-acetyl cis-4-hydroxy-l-proline by coexpression of the Rhizobium l-proline cis-4-hydroxylase and the yeast N-acetyltransferase Mpr1
TM Bach, R Hara, K Kino, I Ohtsu, N Yoshida, H Takagi |
Applied Microbiology and Biotechnology | 2012 |
Inhibition of collagen fibril formation
A Steplewski, A Fertala |
Fibrogenesis & Tissue Repair | 2012 |
Oxygen distribution in the human eye: relevance to the etiology of open-angle glaucoma after vitrectomy
CJ Siegfried, YB Shui, NM Holekamp, F Bai, DC Beebe |
Investigative ophthalmology & visual science | 2010 |
Effects of long-term low-dose oxygen supplementation on the epithelial function, collagen metabolism and interstitial fibrogenesis in the guinea pig lung
T Aoki, F Yamasawa, T Kawashiro, T Shibata, A Ishizaka, T Urano, Y Okada |
Respiratory Research | 2008 |
Collagen Fibril Formation: A NEW TARGET TO LIMIT FIBROSIS
HJ Chung, A Steplewski, KY Chung, J Uitto, A Fertala |
The Journal of biological chemistry | 2008 |
A kampo medicine, Yin-Chiao-san, Prevents bleomycin-induced pulmonary injury in rats
FL Yen, TH Wu, CW Liao, CC Lin |
Phytotherapy Research | 2007 |
Chronic Lung Disease in Early Infancy
D Riley |
Chronic Lung Disease in Early Infancy | 1999 |
Pitfalls in Antiprotease Therapy of Emphysema
GL Snider, EC Lucey, PJ Stone |
American journal of respiratory and critical care medicine | 1994 |
Conjugates of cis-4-Hydroxy-L-proline and Poly(PEG-Lys), a Water Soluble Poly(ether urethane): Synthesis and Evaluation of Antifibrotic Effects in vitro and in vivo
GJ Poiani, DJ Riley, JD Fox, JE Kemnitzer, KF Gean, J Kohn |
Bioconjugate Chemistry | 1994 |
Effects of cis-4-hydroxy-L-proline on the androgen-lnduced growth of the prostate of the prepubertally castrated guinea pig
YC Wong, FL Chan |
The Prostate | 1993 |
Antifibrotic effect of a proline analogue delivered in liposomes to cells in culture
G J Poiani, K F Gean, J D Fox, J Kohn, D J Riley |
Amino Acids | 1993 |
Emphysema: The First Two Centuries-and Beyond: A Historical Overview, with Suggestions for Future Research: Part
GL Snider |
American journal of respiratory and critical care medicine | 1992 |
Putative Role of Neutrophil Elastase in the Pathogenesis of Emphysema
GL Snider, DE Ciccolella, SM Morris, PJ Stone, EC Lucey |
Annals of the New York Academy of Sciences | 1991 |
Synthesis and characterization of magnetically responsive albumin microspheres containingcis-hydroxyproline for scar inhibition
JP Iannotti, TC Baradet, M Tobin, A Alavi, M Staum |
Journal of Orthopaedic Research | 1991 |
Strain specific respiratory air space enlargement in aged rats
JS Kerr, SY Yu, DJ Riley |
Experimental Gerontology | 1990 |
Exacerbation of doxorubicin cardiotoxicity by digoxin administration in an experimental rabbit model
WC Reeves, JW Griffith, MA Wood, L Whitesell |
International Journal of Cancer | 1990 |
Organization and Assembly of Plant and Animal Extracellular Matrix
NA Guzman, GC Fuller, JE Dixon |
Organization and Assembly of Plant and Animal Extracellular Matrix | 1990 |
El pulmón como órgano diana del estrés oxidativo. radicales libres y especies activas del oxígeno
DR Alvira, AS Ariño, FC Lambán, MM Villacampa, MP Martin |
Archivos de Bronconeumología | 1990 |
Amino Acids
G Lubec, GA Rosenthal |
Amino Acids | 1990 |
Molecular, Pharmacologic, and Clinical Aspects of Oxygen-Induced Lung Injury
RM Jackson |
Clinics in Chest Medicine | 1990 |
Pulmonary Strategies of Antioxidant Defense
JE Heffner, JE Repine |
American journal of respiratory and critical care medicine | 1989 |
Long-term Pulmonary Sequelae after Legionella pneumophila Infection in the Hamster
CM Parenti, SW Richards, JR Hoidal, DE Niewoehner |
American journal of respiratory and critical care medicine | 1989 |
Effects of cis-4-hydroxy-l-proline, an inhibitor of Schwann cell differentiation, on the secretion of collagenous and noncollagenous proteins by Schwann cells
CF Eldridge, RP Bunge, MB Bunge |
Experimental Cell Research | 1988 |
Treatment of experimental silicosis with antifibrotic agents
LS Geismar, JS Kerr, RL Trelstad, DJ Riley |
Toxicology | 1988 |
Cadmium-Chloride-induced Air-Space Enlargement with Interstitial Pulmonary Fibrosis Is Not Associated with Destruction of Lung Elastin: Implications for the Pathogenesis of Human Emphysema
GL Snider, EC Lucey, B Faris, Y Jung-Legg, PJ Stone, C Franzblau |
American journal of respiratory and critical care medicine | 1988 |
Colchicine Suppresses the Release of Fibroblast Growth Factors from Alveolar Macrophages In Vitro : The Basis of a Possible Therapeutic Approach to the Fibrotic Disorders
SI Rennard, PB Bitterman, T Ozaki, WN Rom, RG Crystal |
American journal of respiratory and critical care medicine | 1988 |
Elastase/Antielastase Systems in Pulmonary Diseases
S Umeki, Y Niki, R Soejima |
The American Journal of the Medical Sciences | 1988 |
Repair of chronic hyperoxic lung injury: Changes in lung ultrastructure and matrix
RA Durr, BA Dubaybo, LA Thet |
Experimental and Molecular Pathology | 1987 |
Sequential changes in lung morphology during the repair of acute oxygen-induced lung injury in adult rats
L A Thet, S C Parra, J D Shelburne |
Experimental Lung Research | 1986 |
Oxidant injury of the extracellular matrix: Potential role in the pathogenesis of pulmonary emphysema
DJ Riley, JS Kerr |
Lung | 1985 |
Biochemical and cellular aspects of pulmonary oxygen toxicity
B A.Freeman, AK Tanswell |
Advances in Free Radical Biology & Medicine | 1985 |
Effect of proline analogs on oxygen toxicity-induced pulmonary fibrosis in the rat
DJ Riley, JS Kerr, SY Yu |
Toxicology and Applied Pharmacology | 1984 |
Incorporation of modified amino acids into proteins in vivo
MJ Wilson, DL Hatfield |
Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression | 1984 |
Variability in Lung Collagen Amounts After Prolonged Support of Acute Respiratory Failure
JF Collins, JD Smith, JJ Coalson, WG Johanson |
Chest | 1984 |
Pulmonary Oxygen Toxicity Connective Tissue Changes during Injury and Repair
DJ Riley, JS Kerr, SY Yu, RA Berg, NH Edelman |
Chest | 1983 |
A stereochemical concept for the catalytic mechanism of prolylhydroxylase
HM Hanauske-Abel, V Günzler |
Journal of Theoretical Biology | 1982 |
Adult Respiratory-Distress Syndrome: Changing Concepts of Lung Injury and Repair
JE Rinaldo, RM Rogers |
New England Journal of Medicine | 1982 |
Cellular Biology of the Lung
G Cumming, G Bonsignore |
1982 | |
Lung Fibrosis and Emphysema: Divergent Responses to a Common Injury?
DE Niewoehner, JR Hoidal |
Science | 1982 |
The effect of indomethacin, prednisolone and Cis-4-hydroxyproline on pulmonary fibrosis produced by butylated hydroxytoluene and oxygen
JP Kehrer, H Witschi |
Toxicology | 1981 |
Effect of Cis-4-Hydroxy-L-Proline on Lung Mechanics and Collagen Content of Bleomycin-Treated H amsters
DJ Riley, JS Kerr, RA Berg, B Ianni, NH Edelman, DJ Prockop |
Chest | 1981 |