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Citations to this article

Skeletal Muscle Calcium Metabolism and Contractile Force in Vitamin D-deficient Chicks
D. Pleasure, … , N. Nugent, K. Hitz
D. Pleasure, … , N. Nugent, K. Hitz
Published November 1, 1979
Citation Information: J Clin Invest. 1979;64(5):1157-1167. https://doi.org/10.1172/JCI109569.
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Research Article

Skeletal Muscle Calcium Metabolism and Contractile Force in Vitamin D-deficient Chicks

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Abstract

The myopathy associated with vitamin D deficiency has not been well characterized, and it is not known if weakness is a result of a specific effect of vitamin D deficiency on skeletal muscle. Chicks were raised from hatching on a vitamin D-deficient diet, and by 3 wk of age were hypocalcemic and appeared weak. Tension generated by triceps surae during repetitive stimulation of posterior tibial nerve was significantly less than that developed by chicks given vitamin D3 supplements (309 g tension/g wet weight of triceps surae, SD 60, for vitamin D-deficient chicks; 470, SD 77, for vitamin D3-treated chicks, P < 0.01). Histochemical and electron microscopic examination of skeletal muscles of these chicks showed no abnormalities, and there were no electrophysiologic evidences of motor nerve or neuromuscular junction dysfunction. The concentration of ATP in skeletal muscle of the vitamin D-deficient chicks (5.75 μmol/g wet weight, SD 0.17) was not significantly different from that in vitamin D-treated chicks (5.60, SD 0.50). There was no correlation between strength and serum calcium, serum inorganic phosphate, or skeletal muscle inorganic phosphate. Relaxation of tension after tetanic stimulation was slowed in the vitamin D-deficient chicks (20.6 ms, SD 1.7, vs. 15.4, SD 1.3, in vitamin D-treated chicks and 15.3, SD 1.0, in normal control chicks), and in vitro 45Ca++ transport by sarcoplasmic reticulum from the vitamin D-deficient chicks was reduced. Calcium content of mitochondria prepared from leg muscles of vitamin D-deficient chicks (24 nmol/mg mitochondrial protein, SD 6) was considerably lower than that of mitochondria from normal control chicks (45, SD 8) or from chicks treated with vitamin D for 2 wk or more (66-100, depending upon level and duration of therapy). Treatment of the vitamin D-deficient chicks from hatching with sufficient dietary calcium to produce hypercalcemia did not significantly raise skeletal muscle mitochondrial calcium content (31 nmol/mg mitochondrial protein, SD 7) and did not prevent weakness. These studies demonstrate objective weakness as a result of myopathy in vitamin D-deficient chicks, and provide evidence that vitamin D deficiency has effects on skeletal muscle calcium metabolism not secondary to altered plasma concentrations of calcium and phosphate.

Authors

D. Pleasure, B. Wyszynski, A. Sumner, D. Schotland, B. Feldmann, N. Nugent, K. Hitz

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Total citations by year

Year: 2022 2020 2019 2018 2017 2016 2015 2014 2013 2012 2011 2010 2009 2005 2004 2003 2002 1999 1997 1990 1989 1988 1987 1985 1984 1983 1982 1981 1979 Total
Citations: 1 4 2 2 2 6 2 1 2 4 2 3 1 2 1 3 2 1 1 1 5 2 1 3 3 9 1 3 1 71
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Citations to this article (71)

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