Cyclic changes in blood neutrophil counts of grey collie dogs with cyclic hematopoiesis can be eliminated by daily endotoxin injections. Studies were performed to determine the mechanism whereby endotoxin alters this disease. Bone marrow granulocyte-macrophage progenitor cells (colony-forming cells [CFUc]) showed cyclic variation in the untreated grey collie, which was eliminated by chronic endotoxin treatment (Salmonella typhosa lipopolysaccharide W, 5 microgram/kg per day). Similar cyclic variation in blood CFUc was eliminated by this treatment. Tritiated thymidine suicide of the marrow colony-forming cells failed to show cyclic changes to explain the marked swing in CFUc numbers in untreated grey collies. The thymidine suicide rates were not significantly changed by chronic endotoxin treatment. Similarly, serum colony-stimulating activity did not show cyclic variation with the cyclic neutrophil counts in untreated grey collies and was not altered by chronic endotoxin treatment. We suggest that endotoxin eliminates neutrophil cycling in cyclic hematopoiesis by a direct effect on the flux of pluripotent stem cells into the committed stem cell compartment and that this occurs independent of changes in serum colony-stimulating activity.
W P Hammond, E R Engelking, D C Dale
Title and authors | Publication | Year |
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Chronic neutropenia. A new canine model induced by human granulocyte colony-stimulating factor
WP Hammond, E Csiba, A Canin, H Hockman, LM Souza, JE Layton, DC Dale |
Journal of Clinical Investigation | 1991 |
Synchrony of bone marrow proliferation and maturation as the origin of cyclic haemopoiesis
S Schmitz, M Loeffler, JB Jones, RD Lange, HE Wichmann |
Cell Proliferation | 1990 |
Myelopoiesis and Marrow Adherent Cells in Estradiol-Treated Mice
SD Gaunt, KR Pierce |
Veterinary pathology | 1985 |
Canine cyclic hematopoiesis is associated with abnormal purine and pyrimidine metabolism
WR Osborne, WP Hammond, DC Dale |
Journal of Clinical Investigation | 1983 |
Mechanism of canine cyclic hematopoiesis: The role of prostaglandin E in feedback regulation
WP Hammond, RP Robertson, DC Dale |
American Journal of Hematology | 1983 |
Clinical and pathologic features of cyclic hematopoiesis in grey collie dogs
RF DiGiacomo, WP Hammond, LL Kunz, PA Cox |
The American Journal of Pathology | 1983 |
Canine cyclic haematopoiesis: the effect of endotoxin on erythropoiesis
WP Hammond, JW Adamson, DC Dale, E Rodger, C Wise |
British Journal of Haematology | 1982 |
Immunologic Defects in Laboratory Animals 1
ME Gershwin, B Merchant |
1981 | |
The role of colony-stimulating factor in granulopoiesis
RK Shadduck, G Pigoli, A Waheed, F Boegel |
Journal of Supramolecular Structure | 1980 |