Citations to this article

Inhibition of erythrocyte sickling in vitro by pyridoxal.
J A Kark, … , M Woods, L S Lessin
J A Kark, … , M Woods, L S Lessin
Published October 1, 1978
Citation Information: J Clin Invest. 1978;62(4):888-891. https://doi.org/10.1172/JCI109202.
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Research Article

Inhibition of erythrocyte sickling in vitro by pyridoxal.

Abstract

To test the antisickling activity of pyridoxal, we compared the oxygen affinity and the percent sickling at low PO2 of untreated erythrocytes with values for cells from the same blood sample incubated with pyridoxal, glyceraldehyde, or pyridoxine. Pyridoxal increased oxygen affinity much more than glyceraldehyde. 20 mM pyridoxal and glyceraldehyde had equivalent antisickling activity. At PO2 levels above 20 mm Hg, both agents reduced sickling to less than 2%. In samples examined by electron microscopy, pyridoxal reduced the percent sickled cells and the percent cells that contain hemoglobin S fibers by the same amount (from 74 to 3%). Pyridoxine had no effect on oxygen affinity or sockling. Pyridoxal reacts with intracellular hemoglobin to increase oxygen affinity, which inhibits hemoglobin S polymerization and sickling.

Authors

J A Kark, M P Kale, P G Tarassoff, M Woods, L S Lessin

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Year: 2020 2017 2011 2002 1997 1987 1986 1983 1982 1981 Total
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Citations to this article (13)

Title and authors Publication Year
Polymerization of deoxygenated sickle hemoglobin in the presence of fractionated leaf extracts of Anacardium occidentale, Psidium guajava, and Terminalia catappa
PC Chikezie, RC Ekeanyanwu, AB Chile-Agada 		2020
Antioxidant properties and global metabolite screening of the probiotic yeast Saccharomyces cerevisiae var. boulardii: Antioxidant properties and global metabolite screening of probiotic yeast
S Datta, DJ Timson, US Annapure 		Journal of the Science of Food and Agriculture 2017
Sodium metabisulfite-induced polymerization of sickle cell hemoglobin incubated in the extracts of three medicinal plants (Anacardium occidentale, Psidium guajava, and Terminalia catappa)
PC Chikezie 		Pharmacognosy magazine 2011
Metabolic correction in the management of diabetic peripheral neuropathy: improving clinical results beyond symptom control
JR Miranda-Massari, MJ Gonzalez, FJ Jimenez, MZ Allende-Vigo, J Duconge 		Current clinical pharmacology 2011
Vitamin B6 Status of Children With Sickle Cell Disease
MC Nelson, BS Zemel, DA Kawchak, EM Barden, EA Frongillo, SP Coburn, K Ohene-Frempong, VA Stallings 		Journal of Pediatric Hematology/Oncology 2002
Shifts in affinity and enthalpy of oxygen binding to human hemoglobin A induced by pyridoxal and pyridoxal 5′-phosphate
JA Westrick, CR Johnson, JH Simmons, A Smolen 		The Journal of Nutritional Biochemistry 1997
6 Rheology of the sickle cell disorders
J Stuart, CS Johnson 		Baillière's Clinical Haematology 1987
Covalent binding of glutathione to hemoglobin. I. Inhibition of hemoglobin S polymerization
MC Garel, C Domenget, J Caburi-Martin, C Prehu, F Galacteros, Y Beuzard 		The Journal of biological chemistry 1986
Pyridoxal phosphate as an antisickling agent in vitro
JA Kark, PG Tarassoff, R Bongiovanni 		Journal of Clinical Investigation 1983
Schiff base adducts of glyceraldehyde with hemoglobin. Differences in the Amadori rearrangement at the alpha-amino groups
AS Acharya, LG Sussman, JM Manning 		The Journal of biological chemistry 1983
Specific modification of the carboxyl groups of hemoglobin S
R Seetharam, JM Manning, AS Acharya 		The Journal of biological chemistry 1983
A rapid fluorometric assay for erythrocyte pyridoxal kinase
JA Kark, MJ Haut, CU Hicks, CT McQuilkin, RD Reynolds 		Biochemical Medicine 1982
The Molecular Basis of Mutant Hemoglobin Dysfunction
DR Harkness 		The Molecular Basis of Mutant Hemoglobin Dysfunction 1981

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