Citations to this article

Biosynthesis of Chenodeoxycholic Acid in Man: STEREOSPECIFIC SIDE-CHAIN HYDROXYLATIONS OF 5β-CHOLESTANE-3α, 7α-DIOL
S. Shefer, … , G. S. Tint, G. Salen
S. Shefer, … , G. S. Tint, G. Salen
Published September 1, 1978
Citation Information: J Clin Invest. 1978;62(3):539-545. https://doi.org/10.1172/JCI109158.
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Biosynthesis of Chenodeoxycholic Acid in Man: STEREOSPECIFIC SIDE-CHAIN HYDROXYLATIONS OF 5β-CHOLESTANE-3α, 7α-DIOL

Abstract

Stereospecific side-chain hydroxylations of 5β-cholestane-3α, 7α-diol were studied in mitochondrial and microsomal fractions of human liver. Incubation of 5β-cholestane-3α, 7α-diol resulted in hydroxylations at C-12, C-24, C-25, and C-26. Hydroxylations at C-24 and C-26 were accompanied by the introduction of additional asymmetric carbon atoms at C-24 and C-25 respectively, that led to the formation of two distinct pairs of diastereoisomers, namely 5β-cholestane-3α, 7α,24-triols (24R and 24S) and 5β-cholestane-3α, 7α,26-triols (25R and 25S). A sensitive and reproducible radioactive assay to measure the formation of the different biosynthetic 5β-cholestanetriols was developed. Optimal assay conditions for human mitochondrial and microsomal systems were tentatively established.

Authors

S. Shefer, F. W. Cheng, A. K. Batta, B. Dayal, G. S. Tint, G. Salen

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Year: 2013 2005 2002 2001 2000 1997 1996 1990 1985 1984 1983 1982 1981 1980 1979 Total
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Citations to this article (30)

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