In vitro infection with Epstein-Barr virus of bone marrow-derived (B) lymphocytes from blood of patients with X-linked or common varied agammaglobulinemia resulted in the establishment of long-term B-lymphoid cell lines (LCL). LCL were established only with B lymphocytes from patients whose B cells failed to respond to in vitro mitogenic stimulation. In contrast, lymphocytes from patients without small B lymphocytes or with B cells that synthesized but failed to secrete Ig failed uniformly to establish LCL. Analysis of the "nonsecretory" B lymphocytes demonstrated the presence of receptors for C3b and C3d as well as surface Ig, but absence of detectable receptors for Epstein-Barr virus. All of the six cell lines that were established formed rosettes with EAC3 but not with sheep erythrocytes. Four of the six LCL were principally surface IgD bearing and did not synthesize detectable Ig for secretion. Two of the cell lines were indistinguishable from cell lines from normal individuals: they had surface IgG, A, M, and D and synthesized and secreted Ig.
J Schwaber, H Lazarus, F S Rosen
Title and authors | Publication | Year |
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The capacity of interleukin-4 to induce in vitro IgE synthesis by B cells of patients with common variable immunodeficiency
G Pastorelli, MG Roncarolo, C Peronne, PA Tovo, JE Vries |
Clinical & Experimental Immunology | 2008 |
Transcription of germline VH gene elements by normal human fetal liver
N Koenig, B Malone, S Hoch, J Schwaber |
Molecular Immunology | 1997 |
The Primary Immunodeficiencies
FS Rosen, MD Cooper, RJ Wedgwood |
New England Journal of Medicine | 1995 |
The capacity of interleukin-4 to induce in vitro IgE synthesis by B cells of patients with common variable immunodeficiency.
Pastorelli G, Roncarolo MG, Peronne C, Tovo PA, de Vries JE |
Clinical & Experimental Immunology | 1990 |
Interleukin-4 suppresses immunoglobulin production by peripheral blood lymphocytes of patients with common variable immunodeficiency (CVI) induced by supernatants of T cell clones
G Pastorelli, MG Roncarolo, JL Touraine, F Rousset, J Pene, JE de Vries |
Clinical & Experimental Immunology | 1989 |
HLA-DR molecules on all cells are electrophoretically unique
JM Pesando, P Hoffman, MA Stucki |
Leukemia Research | 1989 |
Regulation of the production of secretory and membrane immunoglobulin during lymphocyte development
SL Brown, SL Morrison |
Clinical Immunology and Immunopathology | 1989 |
B lymphocytes from X-linked agammaglobulinemia. Delayed expression of light chain and demonstration of Lyonization in carriers
J Schwaber, J Payne, R Chen |
Journal of Clinical Investigation | 1988 |
Bone Marrow Findings in Immunodeficiency Syndromes
MJ Duggan, DD Weisenburger, NC Sun, DT Purtilo |
Hematology/Oncology Clinics of North America | 1988 |
Human Hybridomas and Monoclonal Antibodies
EG Engleman, SK Foung, JW Larrick, AA Raubitschek |
1985 | |
Human-human hybrids secreting pneumococcal antibodies
JF Schwaber, MR Posner, SF Schlossman, H Lazarus |
Human Immunology | 1984 |
Epstein-Barr virus-induced lymphoblastoid cell lines derived from the peripheral blood of patients with X-linked agammaglobulinemia can secrete IgM
D Levitt, H Ochs, RJ Wedgwood |
Journal of Clinical Immunology | 1984 |
Early pre-B cells from normal and X-linked agammaglobulinaemia produce Cμ without an attached VH region
J Schwaber, H Molgaard, SH Orkin, HJ Gould, FS Rosen |
Nature | 1983 |
Frequency of anti-DNA antibody producing cells from normals and patients with systemic lupus erythematosus
S Hoch, PH Schur, J Schwaber |
Clinical Immunology and Immunopathology | 1983 |
Comparison of a rabbit heteroantiserum and a murine monoclonal antibody raised against a human epithelial ovarian carcinoma cell line
R Berkowitz, S Kabawat, H Lazarus, R Colvin, R Knapp, RC Bast |
American Journal of Obstetrics and Gynecology | 1983 |
Epstein-Barr virus-induced immunoglobulin synthesis by B cells from individuals with late-onset panhypogammaglobulinemia
PL Haber, H Kubagawa, MD Cooper |
Journal of Clinical Immunology | 1983 |
Immature B cells in fetal development and immunodeficiency: Studies of IgM, IgG, IgA and IgD production in vitro using epstein-Barr virus activation
S Pereira, D Webster, T Platts-Mills |
European Journal of Immunology | 1982 |
Isotypes of surface immunoglobulin on B lymphocytes from patients with immune deficiency
JF Schwaber, FS Rosen |
Journal of Clinical Immunology | 1982 |
Reactivity of a monoclonal antibody with human ovarian carcinoma
RC Bast, M Feeney, H Lazarus, LM Nadler, RB Colvin, RC Knapp |
Journal of Clinical Investigation | 1981 |
Deficiency of T Helper Cells in Transient Hypogammaglobulinemia of Infancy
RL Siegel, T Issekutz, J Schwaber, FS Rosen, RS Geha |
New England Journal of Medicine | 1981 |
IgM-restricted production of immunoglobulin by lymphoid cell lines from patients with immunodeficiency with hyper IgM (dysgammaglobulinemia)
JF Schwaber, H Lazarus, FS Rosen |
Clinical Immunology and Immunopathology | 1981 |
Human cell lines express multiple populations of Ia-like molecules
JM Pesando, LM Nadler, H Lazarus, KJ Tomaselli, P Stashenko, J Ritz, H Levine, EJ Yunis, SF Schlossman |
Human Immunology | 1981 |
Epstein-Barr Virus-induced Lymphoblastoid Cell Lines from Patients with Primary Immunodeficiency Diseases
S Tsuchiya, T Konno, K Tada, Y Ono |
Scandinavian Journal of Immunology | 1980 |
Pre-B cells and other possible precursor lymphoid cell lines derived from patients with X-linked agammaglobulinemia
SM Fu, JN Hurley, JM McCune, HG Kunkel, RA Good |
Journal of Experimental Medicine | 1980 |
Functional 'immaturity' of isolated B cells from patients with hypogammaglobulinaemia
GC de Gast, SR Wilkins, AD Webster, A Rickinson, TA Platts-Mills |
Clinical & Experimental Immunology | 1980 |
Predominance of B-lymphocyte function after cultured thymus fragment therapy in severe combined immunodeficiency disease
JF Jones, OF Sieber, VA Fulginiti, H Ochs, H Schulte-Wisserman, R Hong |
Clinical Immunology and Immunopathology | 1980 |
Induction of human immunoglobulin synthesis and secretion in somatic cell hybrids of mouse myeloma and human B lymphocytes from patients with agammaglobulinemia
JF Schwaber, FS Rosen |
Journal of Experimental Medicine | 1978 |