Termination of the Respiratory Burst in Human Neutrophils

Robert C. Jandl; Janine André-Schwartz; Linda Borges-Dubois; Ruby S. Kipnes; B. Jane McMurrich; Bernard M. Babior

doi:10.1172/JCI109033

Termination of the Respiratory Burst in Human Neutrophils

Robert C. Jandl, Janine André-Schwartz, Linda Borges-Dubois, Ruby S. Kipnes, B. Jane McMurrich, and Bernard M. Babior

Published May 1, 1978 - More info

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Abstract

Recent evidence has suggested that a particulate O₂⁻-forming system is responsible for the respiratory burst in activated neutrophils. The respiratory burst is normally a transient event, lasting only 30-60 min. To investigate the mechanism by which the burst is terminated, we examined the O₂⁻-forming activity of neutrophil particles as a function of time in the presence and absence of agents known to affect the function of intact cells. Measurements of the O₂⁻-forming capacity of the particles against time of exposure of neutrophils to opsonized zymosan, a potent stimulating agent, revealed a rapid fall in activity when exposure was continued beyond 3 min. Exposure to zymosan under conditions in which the myeloperoxidase system was inactive (i.e., in the presence of myeloperoxidase inhibitors, or in the absence of oxygen) resulted in a substantial increase in the initial O₂⁻-forming activity of particles from the zymosan-treated cells, but did not prevent the sharp fall in activity seen when zymosan exposure exceeded 10 min. The fall in activity was, however, prevented when activation took place in the presence of cytochalasin B (1.5 μg/ml), an agent thought to act largely by paralyzing the neutrophil through an interaction with its microfilament network.

We conclude from these findings that the termination of the respiratory burst results at least in part from the inactivation of the particulate O₂⁻-forming system. This inactivation involves at least two processes which probably act simultaneously. One is the destruction of the system through the action of myeloperoxidase. The other appears to require active cell motility and is independent of oxygen. The current view holds that the O₂⁻-forming system of the neutrophil is located in the plasma membrane. It may be that the second process involves the internalization and degradation of this membrane-bound system.

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