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Free access | 10.1172/JCI108927
Laboratory of Kidney and Electrolyte Physiology and Departments of Medicine, Peter Bent Brigham Hospital and Harvard Medical School, Boston, Massachusetts 02115
Department of Chemical Engineering, Stanford University, Stanford, California 94305
Departments of Medicine, Harbor General Hospital, Torrance, California 90509
University of California, Los Angeles, California 90024
Find articles by Bohrer, M. in: JCI | PubMed | Google Scholar
Laboratory of Kidney and Electrolyte Physiology and Departments of Medicine, Peter Bent Brigham Hospital and Harvard Medical School, Boston, Massachusetts 02115
Department of Chemical Engineering, Stanford University, Stanford, California 94305
Departments of Medicine, Harbor General Hospital, Torrance, California 90509
University of California, Los Angeles, California 90024
Find articles by Baylis, C. in: JCI | PubMed | Google Scholar
Laboratory of Kidney and Electrolyte Physiology and Departments of Medicine, Peter Bent Brigham Hospital and Harvard Medical School, Boston, Massachusetts 02115
Department of Chemical Engineering, Stanford University, Stanford, California 94305
Departments of Medicine, Harbor General Hospital, Torrance, California 90509
University of California, Los Angeles, California 90024
Find articles by Humes, H. in: JCI | PubMed | Google Scholar
Laboratory of Kidney and Electrolyte Physiology and Departments of Medicine, Peter Bent Brigham Hospital and Harvard Medical School, Boston, Massachusetts 02115
Department of Chemical Engineering, Stanford University, Stanford, California 94305
Departments of Medicine, Harbor General Hospital, Torrance, California 90509
University of California, Los Angeles, California 90024
Find articles by Glassock, R. in: JCI | PubMed | Google Scholar
Laboratory of Kidney and Electrolyte Physiology and Departments of Medicine, Peter Bent Brigham Hospital and Harvard Medical School, Boston, Massachusetts 02115
Department of Chemical Engineering, Stanford University, Stanford, California 94305
Departments of Medicine, Harbor General Hospital, Torrance, California 90509
University of California, Los Angeles, California 90024
Find articles by Robertson, C. in: JCI | PubMed | Google Scholar
Laboratory of Kidney and Electrolyte Physiology and Departments of Medicine, Peter Bent Brigham Hospital and Harvard Medical School, Boston, Massachusetts 02115
Department of Chemical Engineering, Stanford University, Stanford, California 94305
Departments of Medicine, Harbor General Hospital, Torrance, California 90509
University of California, Los Angeles, California 90024
Find articles by Brenner, B. in: JCI | PubMed | Google Scholar
Published January 1, 1978 - More info
To examine the electrostatic effects of fixed negative charges on the glomerular capillary wall, polydisperse [3H]DEAE dextran, a polycationic form of dextran, was infused into 10 Munich-Wistar rats. Fractional clearances of DEAE ranging in radius from 18 to 44Å were determined in these rats, together with direct measurements of the forces and flows governing the glomerular filtration rate of water. These results were compared with data previously obtained in Munich-Wistar rats receiving tritiated neutral dextran (D) and polyanionic dextran sulfate (DS). Measured values for the determinants of the glomerular filtration rate of water in rats given DEAE were found to be essentially identical to those in rats given either D or DS. In addition, DEAE was shown to be neither secreted nor reabsorbed. Fractional clearances of polycationic DEAE were increased relative to both D and DS, the increase relative to D being significant for effective molecular radii ranging from 24 to 44Å.
Fractional DEAE clearances were also measured in a separate group of six Munich-Wistar rats in the early autologous phase of nephrotoxic serum nephritis (NSN). Fractional DEAE clearances in NSN rats were reduced significantly, relative to values measured in normal rats, for effective DEAE radii ranging from 18 to 42Å. Moreover, in NSN rats, fixed negative charges on the glomerular capillary wall were greatly reduced, relative to non-NSN rats, as evidenced by a reduction in intensity of colloidal iron staining. Thus, in NSN rats, DEAE clearances were essentially indistinguishable from values obtained with both neutral D and polyanionic DS.