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Free access | 10.1172/JCI108907
Department of Pediatrics, the Duke University School of Medicine, Durham, North Carolina 27710
Department of Microbiology and Immunology, the Duke University School of Medicine, Durham, North Carolina 27710
Find articles by Sanal, S. in: JCI | PubMed | Google Scholar
Department of Pediatrics, the Duke University School of Medicine, Durham, North Carolina 27710
Department of Microbiology and Immunology, the Duke University School of Medicine, Durham, North Carolina 27710
Find articles by Buckley, R. in: JCI | PubMed | Google Scholar
Published January 1, 1978 - More info
To gain insight into a possible role for antibody-dependent cell-mediated cytotoxicity in vivo, we examined the ability of leukocytes from 28 patients with primary immunodeficiency and from 20 normal controls to lyse three different types of antibody-coated targets in vitro. Mean cytotoxic indices ±1 SD elicited by unfractionated mononuclear cells from normal controls were 28.74±13.26 for human HLA antibody-coated lymphocyte targets, 42.79±8.27 for rabbit IgG antibody-coated chicken erythrocyte targets, and 47.58±10.34 for human anti-CD (Ripley)-coated O+ erythrocyte targets. Significantly (P=<0.05) lower than normal mean cytotoxic indices against lymphocyte targets were seen with effector cells from 10 patients with X-linked agammaglobulinemia (3.7±4.33), in 10 with common variable agammaglobulinemia (16.05±7.74), in 3 with immunodeficiency with hyper IgM (18.41±4.88), and in 2 with severe combined immunodeficiency (3.94±0.3). Antibody-dependent cytotoxicity against chicken erythrocytes was significantly (P=<0.05) lower than normal only in the common variable agammaglobulinemic group (33.33±12.3) and against human erythrocytes only in the common variable (34.36±9.59) and hyper IgM (27.54±0.66) groups. Rosette and anti-F(ab′)2 depletion studies with normal leukocytes indicated that a nonadherent, nonphagocytic, non-Ig-bearing, non-C receptor-bearing, Fc receptor-bearing lymphocyte was the only effector capable of lysing HLA antiboyd-coated lymphocyte targets. Patients with infantile X-linked agammaglobulinemia and severe combined immunodeficiency appear to have a marked deficiency in this type of effector cell function.