Somatostatin- and Epinephrine-Induced Modifications of ⁴⁵Ca⁺⁺ Fluxes and Insulin Release in Rat Pancreatic Islets Maintained in Tissue Culture

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Abstract

The effects of somatostatin and epinephrine have been studied with regard to glucose-induced insulin release and 45Ca++ uptake by rat pancreatic islets after 2 days in tissue culture and with regard to 45Ca++ efflux from islets loaded with the radio-isotope during the 2 days of culture. 45Ca++ uptake, measured simultaneously with insulin release, was linear with time for 5 min. 45Ca++ efflux and insulin release were also measured simultaneously from perifused islets.

Authors

Claes B. Wollheim, Masatoshi Kikuchi, Albert E. Renold, Geoffrey W. G. Sharp