Citations to this article

Immunoinhibition of ristocetin-induced platelet aggregation.
R L Nachman, … , E A Jaffe, B B Weksler
R L Nachman, … , E A Jaffe, B B Weksler
Published January 1, 1977
Citation Information: J Clin Invest. 1977;59(1):143-148. https://doi.org/10.1172/JCI108612.
View: Text | PDF
Research Article

Immunoinhibition of ristocetin-induced platelet aggregation.

Abstract

Human platelets washed and fixed in paraformaldehyde aggregate in the presence of the antibiotic ristocetin and normal plasma. This aggregation response is abolished after digestion of the fixed platelets with chymotrypsin. Antisera to fixed washed platelets were produced in rabbits and absorbed with chymotrypsin-treated, fixed washed platelets. Monovalent Fab fragments obtained from the isolated gamma-globulin fractions of the antisera blocked ristocetin-induced aggregation of fixed washed platelets in buffer and normal platelets in platelet-rich plasma. By double-antibody immunoprecipitation, it was shown that the antibody which blocked the ristocetin reaction interacted with a platelet membrane surface protein of mol wt 155,000. The results suggest that the glycoprotein I complex on the surface of the human platelet mediates ristocetin-induced von Willebrand factor-dependent platelet aggregation.

Authors

R L Nachman, E A Jaffe, B B Weksler

×

Total citations by year

Year: 2017 2013 2008 1998 1993 1991 1990 1989 1988 1986 1985 1984 1983 1982 1981 1980 1979 1978 1977 Total
Citations: 1 1 1 1 1 2 1 1 1 1 4 2 7 4 6 3 9 3 1 50
Citation information

Citations to this article in year 1983 (7)

Title and authors Publication Year
Monoclonal Antibodies against Platelet Membrane Glycoproteins. Characterization and Effect on Platelet Function
JL McGREGOR, J BROCHIER, F WILD, G FOLLEA, MC TRZECIAK, E JAMES, M DECHAVANNE, L McGREGOR, KJ CLEMETSON 		European Journal of Biochemistry 1983
Heparin-binding platelet proteins demonstrated by crossed affinity immunoelectrophoresis
GO Gogstad, NO Solum, MB Krutnes 		British Journal of Haematology 1983
Antibodies against platelet membrane glycoproteins
CS Jenkins, KJ Clemetson, EF Ali-Briggs 		British Journal of Haematology 1983
Potentiation of wheat germ agglutinin aggregation of platelets by von Willebrand protein and by a 116,000 molecular weight tryptic fragment
SE Martin, CW Francis, VJ Marder 		Thrombosis Research 1983
Antibodies against platelet membrane glycoproteins.: III. EFFECT ON THROMBIN- AND BOVINE VON WILLEBRAND FACTOR-INDUCED AGGREGATION
CS Jenkins, KJ Clemetson, EF Ali-Briggs 		British Journal of Haematology 1983
Antiplatelet Agents: Rationale and Results
JG Kelton 		Clinics in Haematology 1983
Purification of human platelet calcium-activated protease. Effect on platelet and endothelial function
N Yoshida, B Weksler, R Nachman 		The Journal of biological chemistry 1983

Advertisement