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Citations to this article

Differences between purified ectopic and normal alpha subnits of human glycoprotein hormones.
B D Weintraub, … , S W Rosen, A S Babson
B D Weintraub, … , S W Rosen, A S Babson
Published October 1, 1975
Citation Information: J Clin Invest. 1975;56(4):1043-1052. https://doi.org/10.1172/JCI108151.
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Research Article

Differences between purified ectopic and normal alpha subnits of human glycoprotein hormones.

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Abstract

"Ectopic" proteins, not distinguished immunologically from the common alpha subunit of the human glycoprotein hormones, were purified approximately 10,000-fold from a gastric carcinoid tumor (A.L.-alpha) and from tissue culture medium of bronchogenic carcinoma cell lines (ChaGo-alpha). The purified A.L.-alpha was homogeneous by sodium dodecyl sulfate (SDS) gel electrophoresis while the purified ChaGo-alpha showed multiple components, some of which represented aggregated species. In SDS gel electrophoresis, the apparent molecular weights of A.L.-alpha (15,000) and dithioerythritol-reduced ChaGo-alpha (13,000) were significantly lower than those of the alpha subunits of human chorionic gonadotropin (hCG-alpha), luteinizing hormone, follicle-stimulating hormone, or thyroid-stimulating hormone (22,000-23,000). Binding experiments with [35S]-SDS suggested that these apparent differences in molecular weight resulted, at least in part, from diminished binding of the SDS by the normal compared to the ectopic alpha subunits. In gel chromatography, the apparent molecular weights of A.L.-alpha (27,000) and ChaGo-alpha (30,000) were slightly higher than those of normal alpha subunits (23,000-24,000). Both A.L.-alpha and ChaGo-alpha were not distinguished from hCG-alpha in ion-exchange chromatography. The composition of A.L.-alpha was similar to that of hCG-alpha in 13 amino acids but showed decreased phenylalanine and increased valine; glucosamine was identified in both A.L.-alpha and hCG-alpha. Under conditions in which hCG-alpha combined with the hCG beta subunit (hCG-beta) to produce 95% of the expected gonadotropin-binding activity in a rat testis radioreceptor-assay, A.L.-alpha incubation with hCG-beta resulted in only 2% of the expected activity, and ChaGo-alpha incubation with hCG-beta produced no detectable activity. These characteristics of ectopic alpha subunits may reflect abnormalities of neoplastic protein synthesis or carbohydrate attachment which result in polypeptides with chemical and immunologic similarity to normal subunits but with differences in physical and combining properties; alternatively, the ectopic subunits may represent as yet unrecognized alpha precursor forms.

Authors

B D Weintraub, G Krauth, S W Rosen, A S Babson

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Total citations by year

Year: 2011 2009 2007 1997 1996 1990 1989 1988 1987 1986 1985 1984 1983 1982 1981 1980 1979 1978 1977 1976 1974 Total
Citations: 1 1 1 2 1 1 1 2 4 2 4 4 5 1 5 6 4 3 2 1 1 52
Citation information
This citation data is accumulated from CrossRef, which receives citation information from participating publishers, including this journal. Not all publishers participate in CrossRef, so this information is not comprehensive. Additionally, data may not reflect the most current citations to this article, and the data may differ from citation information available from other sources (for example, Google Scholar, Web of Science, and Scopus).

Citations to this article (52)

Title and authors Publication Year
Advances in Cancer Research
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Advances in Cancer Research Volume 110 2011
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ZC Chroneos, Z Sever-Chroneos, VL Shepherd
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2009
Non-Gonadotropin-Releasing Hormone-Mediated Transcription and Secretion of Large Human Glycoprotein Hormone α-Subunit in Human Embryonic Kidney-293 Cells
I Casella, H Lindner, C Zenzmaier, D Riitano, P Berger, T Costa
Endocrinology 2007
Characteristics of antigens from an HCGα secreting cell line (ElCo) derived from human breast carcinoma in a Native American patient
RA Pattillo, RO Hussa, MT Story
American Journal of Obstetrics and Gynecology 1997
Disappearance of human chorionic gonadotropin and its α- and β-subunits after term pregnancy
J Korhonen, H Alfthan, P Ylöstalo, J Veldhuis, UH Stenman
Clinical chemistry 1997
Pathophysiological importance of various molecular forms of human choriogonadotropin
H Alfthan, UH Stenman
Molecular and Cellular Endocrinology 1996
N-linked oligosaccharides on free alpha interfere with its ability to combine with human chorionic gonadotropin-beta subunit
DL Blithe
The Journal of biological chemistry 1990
Control of the Thyroid Gland
R Ekholm, LD Kohn, SH Wollman
1989
Pituitary glycoprotein hormone oligosaccharides: Structure, synthesis and function of the asparagine-linked oligosaccharides on lutropin, follitropin and thyrotropin
JU Baenziger, ED Green
Biochimica et Biophysica Acta (BBA) - Reviews on Biomembranes 1988
Purification and characterization of the glycoprotein hormone .alpha.-subunit-like material secreted by HeLa cells
GS Cox, RA Rimerman
Biochemistry 1988
Human chorionic gonadotropin in the rectosigmoid colon. Immunohistochemical study on unbalanced distribution of subunits
M Fukayama, Y Hayashi, M Koike
The American Journal of Pathology 1987
Detection of Early Pregnancy Factor-Like Activity in Women With Gestational Trophoblastic Tumors
AR Mehta, SK Shahani
American Journal of Reproductive Immunology and Microbiology 1987
Molecular Cloning of Hormone Genes
JF Habener
1987
Gonadotropin alpha subunit. Differential processing of free and combined forms in human trophoblast and transfected mouse cells
CL Corless, M Bielinska, TV Ramabhadran, S Daniels-McQueen, T Otani, BA Reitz, DC Tiemeier, I Boime
The Journal of biological chemistry 1987
A single amino acid substitution in an ectopic alpha subunit of a human carcinoma choriogonadotropin
R Nishimura, J Shin, I Ji, CR Middaugh, W Kruggel, RV Lewis, TH Ji
The Journal of biological chemistry 1986
Differential processing of Asn-linked oligosaccharides on pituitary glycoprotein hormones: implications for biologic function
ED Green, I Boime, JU Baenziger
Molecular and Cellular Biochemistry 1986
Production of β-human chorionic gonadotrophin by human squamous carcinoma cell lines
G Cowley, JA Smith, M Ellison, B Gusterson
International Journal of Cancer 1985
INAPPROPRIATE LUTEINIZING HORMONE CONCENTRATION IN HUMAN BREAST CANCER
R Gunasegaram, KL Peh, A Loganath, LC Ang, D Thiagaraj, SR Kottegoda, SS Ratnam
ANZ Journal of Surgery 1985
Proceedings of the 1984 Laurentian Hormone Conference
WF Crowley, M Filicori, DI Spratt, NF Santoro
Proceedings of the 1984 Laurentian Hormone Conference 1985
Contemporary Endocrinology
SH Ingbar
1985
Sulfation of lutropin oligosaccharides with a cell-free system
ED Green, J Gruenebaum, M Bielinska, JU Baenziger, I Boime
Proceedings of the National Academy of Sciences 1984
The effect of protein and RNA synthesis inhibitors on the synthesis and secretion of hCG, α- and β-hCG subunits, in organ culture
H Lorberboum, T Schneider, N Groot, AA Hochberg
European Journal of Obstetrics & Gynecology and Reproductive Biology 1984
Proceedings of the 1983 Laurentian Hormone Conference
IA Kourides, JA Gurr, O Wolf
Proceedings of the 1983 Laurentian Hormone Conference 1984
Free alpha-like material from bovine pituitaries. Removal of its O-linked oligosaccharide permits combination with lutropin-beta
TF Parsons, JG Pierce
The Journal of biological chemistry 1984
HUMAN CHORIONIC GONADOTROPIN IN HUMAN GASTRIC CARCINOMA
H ITO, E TAHARA
Pathology International 1983
Molecular heterogeneity of human chorionic gonadotropin and its subunits in testicular cancer
K Mann, HJ Karl
Cancer 1983
Butyrate regulates glycosylation of the glycoprotein hormone alpha subunit secreted by “glucose-starved” human liver cells
JS Morrow, BD Weintraub, SW Rosen
Biochemical and Biophysical Research Communications 1983
Gonadotropic Hormones
M Sairam
Gonadotropic Hormones 1983
Purification of an alternate form of the alpha subunit of the glycoprotein hormones from bovine pituitaries and identification of its O-linked oligosaccharide
TF Parsons, GA Bloomfield, JG Pierce
The Journal of biological chemistry 1983
Combination of rat lutropin subunits occurs early in the secretory pathway
H Hoshina, I Boime
Proceedings of the National Academy of Sciences 1982
Pure Alpha-Secreting Pituitary Adenomas
EC Ridgway, A Klibanski, PW Ladenson, D Clemmons, IZ Beitins, JW McArthur, MA Martorana, NT Zervas
New England Journal of Medicine 1981
Human chorionic gonadotropin α-subunit in pregnancy
R Benveniste, A Scommegna
American Journal of Obstetrics and Gynecology 1981
Synthesis of the glycoprotein hormone .alpha. subunit and placental alkaline phosphatase by HeLa cells: effect of tunicamycin, 2-deoxyglucose, and sodium butyrate
GS Cox
Biochemistry 1981
Pathologie der endokrinen Organe
E Altenähr, W Bröcker, G Dhom, W Gusek, PU Heitz, G Klöppel, H Lietz, H Mitschke, W Saeger, HJ Schäfer, JJ Staub, H Steiner
1981
A single gonadotropin alpha-subunit gene in normal tissue and tumor-derived cell lines
M Boothby, RW Ruddon, C Anderson, D McWilliams, I Boime
The Journal of biological chemistry 1981
Qualitative and quantitative analyses of human chorionic gonadotropin and its subunits produced by malignant tumors
M Hattori, Y Yoshimoto, S Matsukura, T Fujita
Cancer 1980
Ectopic hormone syndromes
H Imura
Clinics in Endocrinology and Metabolism 1980
SERUM CONCENTRATION OF HUMAN CHORIONIC GONADOTROPIN AND ITS ALPHA AND BETA SUBUNITS. 2. TROPHOBLASTIC TUMOURS
UJ Gaspard, AM Reuter, JL Devìlle, Y Vrindts-Gevaert, KD Bagshawe, P Franchimont
Clinical Endocrinology 1980
SERUM CONCENTRATIONS OF HUMAN CHORIONIC GONADOTROPHIN AND ITS ALPHA AND BETA SUBUNITS. 1. DURING NORMAL SINGLETON AND TWIN PREGNANCIES
AM Reuter, UJ Gaspard, JL Deville, Y Vrindts-Gevaert, P Franchimont
Clinical Endocrinology 1980
Chorionic Gonadotropin
SJ Segal
1980
Synthesis and secretion of human chorionic gonadotropin subunits by cultured human malignant cells
RW Ruddon, CA Hanson, AH Bryan, GJ Putterman, EL White, F Perini, KS Meade, PH Aldenderfer
The Journal of biological chemistry 1980
Synthesis and processing of human chorionic gonadotropin subunits in cultured choriocarcinoma cells
RW Ruddon, CA Hanson, NJ Addison
Proceedings of the National Academy of Sciences 1979
Glycosylation, a variable in the production of hCG by cancers
Y Yoshimoto, AR Wolfsen, WD Odell
The American Journal of Medicine 1979
The synthesis of the glycoprotein hormone α subunit by human breast carcinomas
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European Journal of Cancer (1965) 1979
Placental Proteins
A Klopper, T Chard
1979
Ectopic Secretion of Calcitonin
H Mulder, WH Hackeng
Acta Medica Scandinavica 1978
Precursor—product relationships in the biosynthesis and secretion of thyrotropin and its subunits by mouse thyrotropic tumor cells
BD Weintraub, BS Stannard
FEBS Letters 1978
The Year in Endocrinology 1977
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1978
Clinical relevance of research on the structure of human chorionic gonadotropin
GT Ross
American Journal of Obstetrics and Gynecology 1977
Tumour Markers: Cell Differentiation and the Biological Significance of Inappropriate Tumour Products
ML Ellison
Proceedings of the Royal Society of Medicine 1977
HeLa cells secrete α subunit of glycoprotein tropic hormones
JM Lieblich, BD Weintraub, SW Rosen, JY Chou, JC Robinson
Nature 1976
Current Topics in Experimental Endocrinology
M Jutisz, C Tertrin-Clary
Current Topics in Experimental Endocrinology 1974

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