Citations to this article

Utilization of cultured human lymphoid cells for detection of humoral sensitization in prospective recipients of kidney transplants.
S Ferrone, … , P I Terasaki, R A Reisfeld
S Ferrone, … , P I Terasaki, R A Reisfeld
Published February 1, 1975
Citation Information: J Clin Invest. 1975;55(2):388-394. https://doi.org/10.1172/JCI107942.
View: Text | PDF
Research Article

Utilization of cultured human lymphoid cells for detection of humoral sensitization in prospective recipients of kidney transplants.

Abstract

Prospective recipients of kidney transplants were tested for lymphocytotoxicity; from these we selected 102 sera that lacked cytotoxic antibodies against peripheral lymphocytes from at least 80 unrelated subjects. To detect humoral sensitization, we then reacted these with 17 cultured human lymphoid cell lines having different HL-A phenotypes. Cytotoxic antibodies reacting with these cultured cells were now detected in some of the sera. These antibodies were not directed against HL-A antigens, yet mediated lysis of target cells in the presence of rabbit but not of human or guinea pig complement. Furthermore, they activated the classical pathway of the rabbit complement system. Later, a significant association was found between occurrence of cytotoxic antibodies and rejection of the transplant. Thus, cultured human lymphoid cells, because of their great susceptibility to complement-mediated lysis, appear to be useful in detecting humoral sensitization in candidates for kidney grafts.

Authors

S Ferrone, A Ting, M A Pellegrino, P I Terasaki, R A Reisfeld

×

Total citations by year

Year: 2015 1982 1981 1980 1979 1977 1976 1973 Total
Citations: 1 1 4 2 1 1 2 1 13
Citation information

Citations to this article (13)

Title and authors Publication Year
An Anti-C1s Monoclonal, TNT003, Inhibits Complement Activation Induced by Antibodies Against HLA: TNT003 Blocks HLA-Ab Complement Activation
KA Thomas, NM Valenzuela, D Gjertson, A Mulder, MC Fishbein, GC Parry, S Panicker, EF Reed 		American Journal of Transplantation 2015
An antiglobulin microcytotoxicity assay to analyze non-complement fixing monoclonal antibodies to human histocompatibility antigens
MA Pellegrino, C Russo, AK Ng, AG Pellegrino, S Ferrone 		Journal of Immunological Methods 1982
Ultrastructural alterations in human lymphoblastoid B cell lines treated with tunicamycin
MC Glassy, S Ferrone 		The American Journal of Pathology 1981
Expression of Ia-like antigens on the vasculature of human kidney
PG Natali, CD Martino, M Marcellini, V Quaranta, S Ferrone 		Clinical Immunology and Immunopathology 1981
The monoclonal xenoantibody Q6/64 recognizes a determinant expressed by certain gene products of the A and B loci of the HLA region
V Quaranta, MA Pellegrino, S Ferrone 		Immunogenetics 1981
Cross-reactivity between human and murine lymphocyte antigens: IV. Reactivity of H-2 alloantisera with HLA-A, B antigens
V Quaranta, MA Pellegrino, GN Callahan, S Ferrone 		Immunogenetics 1981
Lymphocytotoxic antibodies in systemic lupus erythematosus patients and their relatives
RP Messner, RD Horatius, S Ferrone 		Arthritis & Rheumatism 1980
Serological, functional, and immunochemical characterization of a monoclonal antibody (MoAb Q2/70) to human Ia-like antigens
V Quaranta, F Indiveri, MC Glassy, A Ng, C Russo, GA Molinaro, MA Pellegrino, S Ferrone 		Human Immunology 1980
Detection of human histocompatibility (HLA) antigens with an indirect rosette microassay
F Indiveri, WB S., MA Pellegrino, S Ferrone 		Journal of Immunological Methods 1979
Cytotoxic Antibodies to Cultured Human Lymphoid Cells in Polytransfused Thrombocytopenic Patients: Association with Reduced Survival of Allogeneic Transfused Platelets
S Ferrone, MA Pellegrino, KK Mittal 		Immunological Investigations 1977
The Major Histocompatibility Complex — Genetics and Biology
FH Bach, JJ van Rood 		New England Journal of Medicine 1976
Expression of C4 on Human Lymphoid Cells and Possible Involvement in Immune Recognition Phenomena
S Ferrone, MA Pellegrino, NR Cooper 		Science 1976
Contemporary Topics in Molecular Immunology
RA Reisfeld, WJ Mandy 		1973

Advertisement