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Citations to this article

Reversal of Ouabain and Acetyl Strophanthidin Effects in Normal and Failing Cardiac Muscle by Specific Antibody
Herman K. Gold, Thomas W. Smith
Herman K. Gold, Thomas W. Smith
Published June 1, 1974
Citation Information: J Clin Invest. 1974;53(6):1655-1661. https://doi.org/10.1172/JCI107716.
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Research Article

Reversal of Ouabain and Acetyl Strophanthidin Effects in Normal and Failing Cardiac Muscle by Specific Antibody

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Abstract

Isolated cat right ventricular papillary muscles were used to study the effects of antibodies with high affinity for ouabain and acetyl strophanthidin on myocardium exposed to these cardioactive steroids. Antibodies with average intrinsic affinity constants for ouabain and acetyl strophanthidin of the order of 108 M-1 were raised in rabbits challenged by repeated injection of a conjugate of ouabain covalently linked to a poly D,L-alanyl derivative of human serum albumin. Effects were assessed in terms of time-course and extent of inotropy reversal, influence of experimentally induced ventricular failure, digitalis-antibody concentration relations, influence of digitalis-antibody complex on response to additionally added digitalis, and relation of antibody effects on digitalis-induced automaticity and contracture to reversal of inotropy. Specific antibody (but not control antibody) in 1.1-1.5-fold molar excess over cardioactive steroid concentrations blocked positive inotropic effects of ouabain and acetyl strophanthidin, and gradually reversed established contractile effects of these agents with a mean time for half-reversal of ouabain-induced inotropy of 124±6 (SEM) min and 37±3 min for half-reversal of acetyl strophanthidin-induced inotropy. Papillary muscles from cats with right ventricular failure induced by chronic pulmonary artery constriction responded similarly. Both normal and failing muscles returned to but not below levels of contractility existing before cardioactive steroid exposure, and time for half-reversal of inotropy by antibody was significantly shorter than time for half-reversal after removal of ouabain or acetyl strophanthidin by muscle bath washout alone. Presence of ouabain- or acetyl strophanthidin-antibody complex did not alter the myocardial contractile response to subsequently added cardioactive steroids.

Authors

Herman K. Gold, Thomas W. Smith

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Total citations by year

Year: 2011 1985 1984 1983 1982 1980 1979 1978 1977 1974 1962 Total
Citations: 1 3 3 1 1 1 1 1 2 2 1 17
Citation information
This citation data is accumulated from CrossRef, which receives citation information from participating publishers, including this journal. Not all publishers participate in CrossRef, so this information is not comprehensive. Additionally, data may not reflect the most current citations to this article, and the data may differ from citation information available from other sources (for example, Google Scholar, Web of Science, and Scopus).

Citations to this article (17)

Title and authors Publication Year
Annual Reports in Medicinal Chemistry
JJ Liu, TW Lee
Annual Reports in Medicinal Chemistry Volume 46 2011
Monoclonal antibodies to T-2 toxin. In vitro neutralization of protein synthesis inhibition and protection of rats against lethal toxemia
G Feuerstein, JA Powell, AT Knower, KW Hunter
Journal of Clinical Investigation 1985
New Advances in the Assessment and Treatment of Digitalis Toxicity
TW Smith
The Journal of Clinical Pharmacology 1985
Digoxin Specific Antibody (Fab) Fragments in 34 Cases of Severe Digitalis Intoxication
A Smolarz, E Roesch, E Lenz, H Neubert, P Abshagen
Journal of Toxicology: Clinical Toxicology 1985
Part III Digitalis glycosides: Mechanisms and manifestations of toxicity
TW Smith, EM Antman, PL Friedman, CM Blatt, JD Marsh
Progress in Cardiovascular Diseases 1984
Part II Digitalis glycosides: Mechanisms and manifestations of toxicity
TW Smith, EM Antman, PL Friedman, CM Blatt, JD Marsh
Progress in Cardiovascular Diseases 1984
Part I Digitalis glycosides: Mechanisms and manifestations of toxicity
TW Smith, EM Antman, PL Friedman, CM Blatt, JD Marsh
Progress in Cardiovascular Diseases 1984
Digoxin-specific antibody fragments and a calcium antagonist for reversal of digoxin-induced mesenteric vasoconstriction
T Hess, G Scholtysik, R Salzmann, W Riesen
Journal of Pharmacy and Pharmacology 1983
Extracellular versus intracellular digoxin action on bovine myocardium, using a digoxin antibody and intracellular glycoside application
P Hess, P Müller
The Journal of Physiology 1982
The effectiveness of digoxin-specific F(ab‘)2-antibody fragments in the treatment of digitoxin poisoning: experimental investigations in the cat
T Hess, G Scholtysik, W Riesen
European Journal of Clinical Investigation 1980
Immunogenicity and kinetics of distribution and elimination of sheep digoxin-specific IgG and Fab fragments in the rabbit and baboon
TW Smith, BL Lloyd, N Spicer, E Haber
Clinical & Experimental Immunology 1979
Contrasting rates of reversal of digoxin toxicity by digoxin-specific IgG and Fab fragments
BL Lloyd, TW Smith
Circulation 1978
Effects of sheep digoxin-specific antibodies and their Fab fragments on digoxin pharmacokinetics in dogs
VP Butler, DH Schmidt, TW Smith, E Haber, BD Raynor, P Demartini
Journal of Clinical Investigation 1977
Reversal of Advanced Digitoxin Toxicity and Modification of Pharmacokinetics by Specific Antibodies and Fab Fragments
HR Ochs, TW Smith
Journal of Clinical Investigation 1977
PRODUCTION AND PROPERTIES OF DIGOXIN-SPECIFIC ANTIBODIES
VP Butler, DH Schmidt, JF Watson, JD Gardner
Annals of the New York Academy of Sciences 1974
DISCUSSION PAPER: USE OF ANTIBODIES IN THE STUDY OF THE MECHANISM OF ACTION OF DIGITALIS
TW Smith
Annals of the New York Academy of Sciences 1974
Cardiac Glycosides
DG Friend
New England Journal of Medicine 1962

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