Citations to this article

Studies on the Mode of Action of Cholera Toxin: EFFECTS ON SOLUBILIZED ADENYLATE CYCLASE
Barbara Beckman, … , Patricia A. Witkum, Geoffrey W. G. Sharp
Barbara Beckman, … , Patricia A. Witkum, Geoffrey W. G. Sharp
Published April 1, 1974
Citation Information: J Clin Invest. 1974;53(4):1202-1205. https://doi.org/10.1172/JCI107660.
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Studies on the Mode of Action of Cholera Toxin: EFFECTS ON SOLUBILIZED ADENYLATE CYCLASE

Abstract

To gain further insight into the mechanism of action of cholera toxin, solubilized preparations of adenylate cyclase from control and toxin-treated rat livers were studied. Adenylate cyclase activity was measured in both particulate and solubilized form in rat liver under control conditions and after intravenous injection of cholera toxin. Cholera toxin caused a 3.3-fold activation of adenylate cyclase in the particulate preparation and a 5.8-fold increase in the solubilized preparation. Thus, the ability of cholera toxin to stimulate adenylate cyclase is present even when the enzyme membrane environment is disrupted. Furthermore, the solubilized enzyme, after treatment with cholera toxin, retained its ability to respond to catecholamines, but not to glucagon. In contrast, the control enzyme lost its responsiveness to catecholamines and glucagon after solubilization.

Authors

Barbara Beckman, Jorge Flores, Patricia A. Witkum, Geoffrey W. G. Sharp

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Total citations by year

Year: 2014 2008 1986 1985 1981 1980 1979 1978 1977 1976 1975 1974 Total
Citations: 1 1 1 1 1 2 2 6 5 5 10 2 37
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Citations to this article (37)

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