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Research Article Free access | 10.1172/JCI107611

Remodeling of Granulocyte Membrane Fatty Acids During Phagocytosis

James E. Smolen and Stephen B. Shohet

Divisions of Hematology, the Department of Medicine, Children's Hospital Medical Center, Harvard Medical School, Boston, Massachusetts 02114

Department of Medicine, Moffitt Hospital, University of California, San Francisco, California 94143

Department of Laboratory Medicine, Moffitt Hospital, University of California, San Francisco, California 94143

San Francisco Medical Center, San Francisco, California 94143

Find articles by Smolen, J. in: PubMed | Google Scholar

Divisions of Hematology, the Department of Medicine, Children's Hospital Medical Center, Harvard Medical School, Boston, Massachusetts 02114

Department of Medicine, Moffitt Hospital, University of California, San Francisco, California 94143

Department of Laboratory Medicine, Moffitt Hospital, University of California, San Francisco, California 94143

San Francisco Medical Center, San Francisco, California 94143

Find articles by Shohet, S. in: PubMed | Google Scholar

Published March 1, 1974 - More info

Published in Volume 53, Issue 3 on March 1, 1974
J Clin Invest. 1974;53(3):726–734. https://doi.org/10.1172/JCI107611.
© 1974 The American Society for Clinical Investigation
Published March 1, 1974 - Version history
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Abstract

During phagocytosis, new phospholipid is synthesized from triglyceride fatty acid and may be utilized to form the membranes of phagocytic vesicles. In addition, hydrogen peroxide, which can peroxidize unsaturated fatty acids, is generated. Since both of these processes could change membrane fatty acid composition during the conversion of cytoplasmic granules and plasma membranes to phagosomes, the lipid compositions of these structures were examined. Phagocytic vesicles were prepared by density gradient centrifugation of polystyrene latex particles after phagocytosis. Granule and plasma membrane fractions were isolated by density gradient and differential centrifugation. Phospholipids and fatty acids were analyzed by thin-layer chromatography and gas-liquid chromatography.

While whole cells, granules, plasma membranes, and phagosomes were all similar in phospholipid composition, phagosome fatty acids were significantly more saturated than those of the other fractions. This was primarily due to reduced oleic and arachidonic acids and increased palmitic acid in the phagocytic vesicle lipids. Plasma membrane was also more saturated in comparison to whole cells and granules. However, this difference was not sufficient to explain the marked comparative saturation of the phagosomes. The observed increase in fatty acid saturation in these lipids may have been induced by a combination of either peroxidative destruction of polyunsaturated fatty acids or phospholipase activity, coupled with reacylation mechanisms favoring saturated fatty acids.

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