High-affinity, limited-capacity nuclear binding activities, putative receptors for triiodothyronine, were detected after incubation of hormone with intact rat pituitary GH1 cells in culture, isolated GH1 cell nuclei, or rat liver nuclei. The total number of triiodothyronine binding sites per nucleus was similar in each case (approximately 8,000). The estimated equilibrium dissociation constants were virtually identical in isolated GH1 cell nuclei and rat liver nuclei, and both values were similar to that determined in intact GH2 cells. These results suggest that mechanisms of thyroid hormone action defined in cell culture could apply to thyroid hormone regulatory effects in vivo.
H H Samuels, J S Tsai
Title and authors | Publication | Year |
---|---|---|
Retinoid-X receptor agonists increase thyroid hormone competence in lower jaw remodeling of pre-metamorphic Xenopus laevis tadpoles
B Mengeling, L Vetter, J Furlow, M Klymkowsky |
PloS one | 2022 |
High rates of (treated) hypothyroidism among chronic migraine patients consulting a specialized headache clinic: are we missing something?
M Filipchuk, J Gassmann, TC Zamparella, MC Tibaldo, M Carpinella, PS Tagliavini, P Scarnato, MT Goicochea, O Bruera, DM Magris, M Lisicki |
Neurological Sciences | 2021 |
Transport, Metabolism, and Function of Thyroid Hormones in the Developing Mammalian Brain
BK Stepien, WB Huttner |
Frontiers in Endocrinology | 2019 |
Role of thyroid hormone in hepatic gene regulation, chromatin remodeling, and autophagy
BK Singh, RA Sinha, K Ohba, PM Yen |
Molecular and Cellular Endocrinology | 2017 |
Thyroid Hormone Receptors in Two Model Species for Vertebrate Embryonic Development: Chicken and Zebrafish
VM Darras, SL van Herck, M Heijlen, BD Groef |
Journal of Thyroid Research | 2011 |
Detection of thyroid hormone receptor disruptors by a novel stable in vitro reporter gene assay
J Freitas, P Cano, C Craig-Veit, ML Goodson, JD Furlow, AJ Murk |
Toxicology in Vitro | 2011 |
Thyroid hormone and apotransferrin regulation of growth hormone secretion by GH1 rat pituitary tumor cells in iron restricted serum-free defined medium
DA Sirbasku, R Pakala, H Sato, JE Eby |
In Vitro Cellular & Developmental Biology - Animal | 1992 |
Thyroid Disease in Clinical Practice
IR McDougall |
1992 | |
Thyroid hormone and apotransferrin regulation of growth hormone secretion by GH1 rat pituitary tumor cells in iron restricted serum-free defined medium
DA Sirbasku, R Pakala, H Sato, JE Eby |
In Vitro Cellular & Developmental Biology – Animal | 1992 |
Role of L-thyroxine in nuclear thyroid hormone receptor occupancy and growth hormone production in cultured GC cells
Y Halperin, LE Shapiro, MI Surks |
Journal of Clinical Investigation | 1991 |
Novel cold-sensitive cytosolic 3,5,3'-triiodo-L-thyronine-binding proteins in human red blood cell. Isolation and characterization
AN Fanjul, RN Farias |
The Journal of biological chemistry | 1991 |
Oligomeric binding of T3 receptor is required for maximal T3 response
GR Williams, JW Harney, BM Forman, HH Samuels, GA Brent |
The Journal of biological chemistry | 1991 |
Thyroid hormone 5′-deiodinase activity, nuclear binding, and effects on mitogenesis in umr-106 osteoblastic osteosarcoma cells
BA Lebron, AE Pekary, C Mirell, TJ Hahn, JM Hershman |
Journal of Bone and Mineral Research | 1989 |
Thyroid Hormone Receptors and Action: The 5′-Flanking Region of the Rat Growth Hormone Gene can Mediate Regulated Gene Expression
HH Samuels, J Casanova, RP Copp, L Janocko, BM Raaka, H Sahnoun, BM Yaffe |
Endocrine Research | 1989 |
Rat pancreatic nuclear thyroid hormone receptor: Characterization and postnatal development
JT Lee, E Lebenthal, PC Lee |
Gastroenterology | 1989 |
Purification and Characterization of NADPH-dependent Cytosolic 3,5,3′-Triiodo-L-thyronine Binding Protein in Rat Kidney
K Hashizume, T Miyamoto, K Ichikawa, K Yamauchi, M Kobayashi, A Sakurai, H Ohtsuka, Y Nishii, T Yamada |
The Journal of biological chemistry | 1989 |
Regulation of gene expression by thyroid hormone
HH Samuels, BM Forman, ZD Horowitz, ZS Ye |
Journal of Clinical Investigation | 1988 |
Thyroid hormone resistance in hibernating ground squirrels, Spermophilus richardsoni
TH Magnus, NE Henderson |
General and Comparative Endocrinology | 1988 |
Proceedings of the 1987 Laurentian Hormone Conference
RB Jaffe, JJ Mulchahey, AM di Blasio, MC Martin, Z Blumenfeld, DA Dumesic |
Proceedings of the 1987 Laurentian Hormone Conference | 1988 |
Decreased hindlimb skeletal muscle weight and nuclear T3 receptor binding of obese (ob/ob) mice
EG Gilvary, PA Lachance, SG Khan |
Nutrition Research | 1988 |
Photoaffinity labeling of thyroid hormone receptors
ZD Horowitz, HH Samuels |
Pharmacology & Therapeutics | 1987 |
Nuclear receptors for l-triiodothyronine in trout erythrocytes
CV Sullivan, DS Darling, WW Dickhoff |
General and Comparative Endocrinology | 1987 |
n-Butyrate increases the level of thyroid hormone nuclear receptor in non-pituitary cultured cells
T Mitsuhashi, H Uchimura, F Takaku |
The Journal of biological chemistry | 1987 |
Modulation of myocardial L-triiodothyronine receptors in normal, hypothyroid, and hyperthyroid rats
PW Ladenson, JD Kieffer, AP Farwell, EC Ridgway |
Metabolism | 1986 |
Nuclear thyroid hormone receptors in cultured human fibroblasts: Improved method of isolation, partial characterization, and interaction with chromatin
K Ichikawa, LJ DeGroot, S Refetoff, AL Horwitz, ER Pollak |
Metabolism | 1986 |
Nuclear thyroid hormone receptors in cultured bone cells
R Rizzoli, J Poser, U Bürgi |
Metabolism | 1986 |
Preliminary comparative nuclear T3 receptor binding characteristics in liver and skeletal muscle of the obese mouse and Zucker fatty rat
SG Khan, PC Boyle, PA Lachance |
Nutrition Research | 1986 |
Hormones and Lung Maturation
PL Ballard |
1986 | |
Frontiers in Thyroidology
G Medeiros-Neto, E Gaitan |
1986 | |
Enzymes of Lipid Metabolism II
L Freysz, H Dreyfus, R Massarelli, S Gatt |
1986 | |
Clinical Endocrinology
A Labhart |
Clinical Endocrinology | 1986 |
Loss of thyroidal inducibility of Na,K-ATPase with neoplastic transformation in tissue culture
DL Guernsey, IS Edelman |
The Journal of biological chemistry | 1986 |
Solubilization of L-triiodothyronine binding site from human erythrocyte membrane
JA Botta, RN Farías |
Biochemical and Biophysical Research Communications | 1985 |
Thyroid hormone action: determination of hormone-receptor interaction using structural analogs and molecular modeling
EN Cheung |
Trends in Pharmacological Sciences | 1985 |
Uptake of radiolabelled triiodothyronine in the brain of steelhead trout (Salmo gairdneri) during parr-smolt transformation: Implications for the mechanism of thyroid activation of olfactory imprinting
AT Scholz, RJ White, M Muzi, T Smith |
Aquaculture | 1985 |
Biochemical Actions of Hormones
D Marver |
Biochemical Actions of Hormones | 1985 |
Nuclear triiodothyronine binding sites in rat adipocytes
U Buergi, U Abbuehl |
Metabolism | 1984 |
Modulation of adenovirus transformation by thyroid hormone
PB Fisher, DL Guernsey, IB Weinstein, IS Edelman |
Proceedings of the National Academy of Sciences | 1983 |
Effects of various pathological conditions on the nuclear T3 receptors of rat cerebral cortex: comparison with liver
CL Thrall |
Brain Research | 1983 |
New Comprehensive Biochemistry
S Yamamoto |
New Comprehensive Biochemistry | 1983 |
Molecular Basis of Thyroid Hormone Action
P Larsen, J Silva |
Molecular Basis of Thyroid Hormone Action | 1983 |
High affinity L-triiodothyronine binding sites on washed rat erythrocyte membranes
JA Botta, D de Mendoza, RD Morero, RN Farías |
The Journal of biological chemistry | 1983 |
Hormonal Regulation of Growth Hormone mRNA
M Wegnez, B Schachter, J Baxter, J Martial |
DNA | 1982 |
A new unextracted-sample radioimmunoassay method for hepatic endogenous nuclear L-tri-iodothyronine content. Validity of its use in determining nuclear receptor binding characteristics
T Yagura, PG Walfish |
Biochemical Journal | 1982 |
Impaired repair capacity of DNA strand breaks induced by 125I-triiodothyronine in Chinese hamster cells
S Sundell-Bergman, KJ Johanson |
Biochemical and Biophysical Research Communications | 1982 |
Reciprocal effects of an acute load of thyroxine or triiodothyronine on their peripheral metabolism and deiodination in the cold-acclimated rat
R Boado, E Ulloa, AA Zaninovich |
Journal of Endocrinological Investigation | 1982 |
Interaction of drugs with calmodulin
B Weiss, WC Prozialeck, TL Wallace |
Biochemical Pharmacology | 1982 |
The size of the thyroid hormone receptor in chromatin
DJ Gruol, ES Kempner |
The Journal of biological chemistry | 1982 |
Triiodothyronine and Adipose Conversion of OB17Preadipocytes: Binding to High Affinity Sites and Effects on Fatty Acid Synthetizing and Esterifying Enzymes
J Gharbi-chihi, P Grimaldi, J Torresani, GÉ Ailhaud |
Journal of Receptors and Signal Transduction | 1981 |
Specific triiodothyronine binding by tumor cells and spleen cells in a thyroid hormone dependent mouse tumor system
MK Gupta, T Chiang, SD Deodhar |
European Journal of Cancer and Clinical Oncology | 1981 |
Corticosteroids in pregnancy: Their potential hazards and track record
BP Sachs |
Journal of Obstetrics & Gynaecology | 1981 |
Nuclear binding of T3 and effects of QO2, Na-K-ATPase, and alpha-GPDH in liver and kidney
D Somjen, F Ismail-Beigi, IS Edelman |
American journal of physiology. Endocrinology and metabolism | 1981 |
Development of support matrices for affinity chromatography of thyroid hormone receptors
KR Latham, JW Apriletti, NL Eberhardt, JD Baxter |
The Journal of biological chemistry | 1981 |
Fatty acid synthesis in fetal lung
DK Das |
Biochemical and Biophysical Research Communications | 1980 |
Specific thyroxine receptors in mammary cytosol from lactating cattle
DB Wilson, RC Gorewit |
Biochemical and Biophysical Research Communications | 1980 |
Proceedings of the 1979 Laurentian Hormone Conference
DT Krieger, A Liotta, M Brownstein, E Zimmerman |
Proceedings of the 1979 Laurentian Hormone Conference | 1980 |
Relationship of receptor affinity to the modulation of thyroid hormone nuclear receptor levels and growth hormone synthesis by L-triiodothyronine and iodothyronine analogues in cultured GH1 cells
HH Samuels, F Stanley, J Casanova |
Journal of Clinical Investigation | 1979 |
Regulation of the Conversion of Thyroxine to Triiodothyronine in the Perfused Rat Liver
AS Jennings, DC Ferguson, RD Utiger |
Journal of Clinical Investigation | 1979 |
Dissociable and non-dissociable cytoplasmic protein-thyroid hormone interactions
K Yoshida, PJ Davis, M Schoenl |
Biochimica et Biophysica Acta (BBA) - General Subjects | 1979 |
In vitro triiodothyronine binding to cytoplasmic proteins from human red blood cells
J Michelot, B Dastugue, N Defer, G Meyniel |
Biochemical and Biophysical Research Communications | 1979 |
In vivo demonstration of the presence of specific saturable binding sites for triiodothyronine in nuclei of tadpole liver
E Toth, M Tabachnick |
General and Comparative Endocrinology | 1979 |
Na+ pump activity and nuclear T3 receptors in tissues of genetically obese () mice
DL Guernsey, WK Morishige |
Metabolism | 1979 |
Non-thyrotropic pituitary cells in culture convert T4 to T3
S Melmed, G Kurtzman, A Reed, JH Hershman |
Life Sciences | 1979 |
Evidence of specific nuclear binding sites for T3 in the mouse cultured fibroblast
A Brisson-Lougarre, S Jozan, C Blum |
Journal of Endocrinological Investigation | 1979 |
Hormone Receptors
JD Baxter, JW Funder |
New England Journal of Medicine | 1979 |
Receptor affinity and biological potency of thyroid hormones in thyrotropic cells
MC Gershengorn, E Geras, BE Marcus-Samuels, MJ Rebecchi |
American journal of physiology. Endocrinology and metabolism | 1979 |
Thyroid Hormone Action at the Cellular Level
JH Oppenheimer |
Science | 1979 |
Contributions of Plasma Triiodothyronine and Local Thyroxine Monodeiodination to Triiodothyronine to Nuclear Triiodothyronine Receptor Saturation in Pituitary, Liver, and Kidney of Hypothyroid Rats FURTHER EVIDENCE RELATING SATURATION OF PITUITARY NUCL
JE Silva, PR Larsen |
Journal of Clinical Investigation | 1978 |
Properties of solubilized nuclear triiodothyronine binding proteins
J Torresani, A Anselmet, R Wahl |
Molecular and Cellular Endocrinology | 1978 |
Nuclear triiodothyronine receptors in the developing rat brain
T Valcana, PS Timiras |
Molecular and Cellular Endocrinology | 1978 |
Molecular mechanisms at the tissue level in hyperthyroidism
JH Oppenheimer, WH Dillmann |
Clinics in Endocrinology and Metabolism | 1978 |
Receptors and Hormone Action
AW Norman, WR Wecksler |
Receptors and Hormone Action | 1978 |
Klinik der inneren Sekretion
A Labhart |
1978 | |
Thyroid Hormonelike Actions of 3,3′,5′- SS Papavasiliou, JA Martial, KR Latham, JD Baxter |
Journal of Clinical Investigation | 1977 |
High affinity thyroid hormone binding sites on purified rat liver plasma membranes
NB Pliam, ID Goldfine |
Biochemical and Biophysical Research Communications | 1977 |
Thyroid hormone stimulates de novo growth hormone synthesis in cultured GH1 cells: evidence for the accumulation of a rate limiting RNA species in the induction process
HH Samuels, LE Shapiro |
Proceedings of the National Academy of Sciences | 1976 |
Dose-dependent depletion of nuclear receptors by L-triiodothyronine: evidence for a role in induction of growth hormone synthesis in cultured GH1 cells
HH Samuels, F Stanley, LE Shapiro |
Proceedings of the National Academy of Sciences | 1976 |
Binding of insulin to isolated nuclei
ID Goldfine, GJ Smith |
Proceedings of the National Academy of Sciences | 1976 |
The uptake and distribution of radiolabelled thyroxine by isolated cardiac myocytes
AH Burns, WJ Reddy |
Life Sciences | 1976 |
Absence of high-affinity low-capacity components of T4-binding sites in the cytosol of the bullfrog tadpole
K Yoshizato, A Kistler, N Shioya |
General and Comparative Endocrinology | 1976 |
Proceedings of the 1975 Laurentian Hormone Conference
J Genest, W Nowaczynski, O Kuchel, R Boucher, J Rojo-Ortega, G Constantopoulos, D Ganten, F Messerli |
Proceedings of the 1975 Laurentian Hormone Conference | 1976 |
Hormone—receptor interactions in brain: Uptake and binding of thyroid hormone
NL Eberhardt, T Valcana, PS Timiras |
Psychoneuroendocrinology | 1976 |
Thyroid Hormones: Effect of Physiological Concentrations on Cultured Cardiac Cells
JS Tsai, A Chen |
Science | 1976 |
In vitro binding of L-triiodothyronine to receptors in rat liver nuclei. Kinectics of binding, extraction properties, and lack of requirement for cytosol proteins
MI Surks, DH Koerner, JH Oppenheimer |
Journal of Clinical Investigation | 1975 |
PERIPHERAL RESISTANCE TO THYROXINE: A CAUSE OF SHORT STATURE IN A BOY WITHOUT GOITRE
G Schneider, HR Keiser, CW Bardin |
Clinical Endocrinology | 1975 |
Thyroid hormone binding by a component of mitochondrial membrane
K Sterling, PO Milch |
Proceedings of the National Academy of Sciences | 1975 |
Nuclear receptors for thyroid hormone: evidence for nonrandom distribution within chromatin
MA Charles, GU Ryffel, M Obinata, BJ McCarthy, JD Baxter |
Proceedings of the National Academy of Sciences | 1975 |
Nuclear thyroid hormone receptors: Evidence for association with nucleolar chromatin
RS Gardner |
Biochemical and Biophysical Research Communications | 1975 |
DNA binding of thyroid hormone receptors
KM MacLeod, JD Baxter |
Biochemical and Biophysical Research Communications | 1975 |
Triiodothyronine binding proteins in rat liver cytosol
N Defer, B Dastugue, MM Sabatier, P Thomopoulos, J Kruh |
Biochemical and Biophysical Research Communications | 1975 |
Initiation of Thyroid-Hormone Action
HL Bleich, ES Boro, JH Oppenheimer |
New England Journal of Medicine | 1975 |
The Peripheral Action of the Thyroid Hormones
JH Oppenheimer, MI Surks |
Medical Clinics of North America | 1975 |
Nuclear Binding Capacity Appears to Limit the Hepatic Response to L-Triiodothyronine (T 3 )
JH Oppenheimer, HL Schwartz, MI Surks |
Endocrine Research | 1975 |
Thyroid hormone receptors. Binding characteristics and lack of hormonal dependency for nuclear localization
BJ Spindler, KM MacLeod, J Ring, JD Baxter |
The Journal of biological chemistry | 1975 |
Binding of selected iodothyronine analogues to receptor sites of isolated rat hepatic nuclei. High correlation between structural requirements for nuclear binding and biological activity
D Koerner, HL Schwartz, MI Surks, JH Oppenheimer |
The Journal of biological chemistry | 1975 |
Thyroid Hormone Action IN VITRO CHARACTERIZATION OF SOLUBILIZED NUCLEAR RECEPTORS FROM RAT LIVER AND CULTURED GH1 CELLS
HH Samuels, JS Tsai, J Casanova, F Stanley |
Journal of Clinical Investigation | 1974 |
The thyroid and the kidney
SE Bradley, F Stéphan, JB Coelho, P Réville |
Kidney International | 1974 |
In vitro triiodothyronine binding to non-histone proteins from rat liver nuclei
P Thomopoulos, B Dastugue, N Defer |
Biochemical and Biophysical Research Communications | 1974 |
Physical Properties of a Dog Liver and Kidney Cytosol Protein That Binds Thyroid Hormone
PJ Davis, BS Handwerger, F Glaser |
The Journal of biological chemistry | 1974 |
Thyroid Hormone Action: In vitro Demonstration of Putative Receptors in Isolated Nuclei and Soluble Nuclear Extracts
HH Samuels, JS Tsai, J Casanova |
Science | 1974 |