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Citations to this article

Stimulation of human neutrophil leukocyte aerobic glucose metabolism by purified chemotactic factors.
E J Goetzl, K F Austen
E J Goetzl, K F Austen
Published February 1, 1974
Citation Information: J Clin Invest. 1974;53(2):591-599. https://doi.org/10.1172/JCI107594.
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Research Article

Stimulation of human neutrophil leukocyte aerobic glucose metabolism by purified chemotactic factors.

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Abstract

The interaction of human neutrophils adherent to plastic petri dishes with the purified chemotactic factors C5a and kallikrein increased their rate of aerobic glycolysis 25-120% and the activity of their hexose monophosphate shunt (HMPS) 100-600%, reaching a plateau after 2 hr at 37 degrees C. The stimulation of either pathway required a chemotactically active stimulus since neither C5 nor prekallikrein or inactivated kallikrein could enhance metabolic activity. Marked suppression of the neutrophil chemotactic response by preincubation with a chemotactic factor to achieve deactivation, 5 x 10(-7) M diisopropyl fluorophosphate, or the neutrophil immobilizing factor (NIF) did not prevent the stimulation of HMPS activity or glycolysis by chemotactic factors. The metabolic inhibitors iodoacetate and 6-aminonicotinamide at concentrations which blocked enhancement of glycolysis or HMPS activity, respectively, partially suppressed the chemotactic response of neutrophils to the chemotactic factors. The capacity of a chemotactic factor to stimulate glucose metabolism of human neutrophils is associated with a maximal chemotactic response, but this stimulation is not alone sufficient for chemotaxis.

Authors

E J Goetzl, K F Austen

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Year: 2022 2019 2018 2015 2013 2012 2011 2009 2008 2006 2004 2003 2002 1999 1998 1997 1996 1995 1994 1993 1991 1990 1989 1988 1987 1986 1985 1984 1983 1982 1981 1980 1979 1978 1977 1976 1975 1974 Total
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