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Citations to this article

Factors governing the transepithelial potential difference across the proximal tubule of the rat kidney.
L J Barratt, … , J P Kokko, D W Seldin
L J Barratt, … , J P Kokko, D W Seldin
Published February 1, 1974
Citation Information: J Clin Invest. 1974;53(2):454-464. https://doi.org/10.1172/JCI107579.
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Research Article

Factors governing the transepithelial potential difference across the proximal tubule of the rat kidney.

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Abstract

Previous measurements of the transepithelial potential difference (PD) of the proximal tubule have yielded widely conflicting values (range -20 to +3 mV). In a recent study, Kokko has demonstrated that the PD of the in vitro perfused isolated proximal tubule of the rabbit varies in a predictable way from -6 to +3 mV, depending on the concentration of chloride, bicarbonate, glucose, and amino acids in the perfusing solution. The present micropuncture study examines the effect of tubular fluid composition on the PD profile along the proximal tubule of the in vivo rat kidney. Low resistance measuring electrodes with large tips (3-5 microns OD) filled with 3 M KCl, were used to provide stable PD recordings. Experiments were performed to validate the use of these electrodes. Transepithelial PD measurements were made in immediate postglomerular segments identified by injection of dye into Bowman's space of accessible surface glomeruli and in randomly selected more distal segments of the proximal tubule. In the control state, the first loop was found to have a small but consistently negative PD which could be obliterated by an infusion of phloridzin. In contrast, the PD in later segments was consistently positive. Infusion of acetazolamide abolished the positive PD in the later segments. Acetazolamide and glucose infusion resulted in a negative PD which was abolished by the additional infusion of phloridzin. These data provide evidence that glucose reabsorption is electrogenic and can account for the small negative PD normally present in the early proximal tubule. The positive PD in later segments appears to be a passive chloride diffusion potential. This positive potential is discussed as an important electrochemical driving force for significant passive reabsorption of sodium in the proximal tubule.

Authors

L J Barratt, F C Rector Jr, J P Kokko, D W Seldin

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Total citations by year

Year: 2024 2020 2019 2017 2016 2015 2014 2013 2012 2009 2003 2002 1998 1989 1987 1986 1985 1984 1983 1982 1981 1980 1979 1978 1977 1976 1975 1974 1969 Total
Citations: 2 1 1 5 2 1 3 1 5 1 2 1 1 1 1 2 3 1 3 2 8 3 6 6 9 15 6 2 1 95
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