Citations to this article

Biosynthesis of Proparathyroid Hormone and Parathyroid Hormone by Human Parathyroid Glands
Luke L. H. Chu, … , James W. Hamilton, David V. Cohn
Luke L. H. Chu, … , James W. Hamilton, David V. Cohn
Published December 1, 1973
Citation Information: J Clin Invest. 1973;52(12):3089-3094. https://doi.org/10.1172/JCI107508.
View: Text | PDF
Research Article

Biosynthesis of Proparathyroid Hormone and Parathyroid Hormone by Human Parathyroid Glands

Abstract

Human parathyroid glands obtained at autopsy were incubated with [3H]leucine and [3H]lysine. After incubation, nonradioactive parathyroid tissue of either human or bovine origin was added. Radioactive parathyroid hormone and proparathyroid hormone were isolated from the gland and medium by organic solvent and salt fractionation, trichloroacetic acid precipitation, Sephadex G-100 gel filtration, and carboxymethyl cellulose column chromatography. The human hormonal peptides were identified in the ion-exchange column eluates by their relatively high levels of radioactivity, their elution positions, and their immunoreactivity to anti-PTH antiserum. The time-course of radioactive amino acid incorporation into these peptides and a brief incubation of the gland with radioactive amino acids, followed by various lengths of incubation with nonradioactive amino acids, indicated that a precursor-product relationship exists for the two peptides. An alternate method for isolation of the hormone and prohormone, which involves separation of peptides by urea-polyacrylamide gel electrophoresis, confirmed the identities of the human parathyroid hormone and proparathyroid hormone.

Authors

Luke L. H. Chu, Ronal R. MacGregor, Paul I. Liu, James W. Hamilton, David V. Cohn

×

Total citations by year

Year: 2010 2008 2002 1996 1983 1981 1980 1978 1977 1976 1975 1974 Total
Citations: 1 1 1 1 1 2 1 2 4 4 6 5 29
Citation information

Citations to this article (29)

Title and authors Publication Year
Vitamins & Hormones
KJ Hare, FK Knop 		Vitamins & Hormones 2010
Novartis Foundation Symposia
EJ Campbell 		Ciba Foundation Symposium 44 - Research and Medical Practice: Their Interaction 2008
Biosynthesis and Secretion of Parathyroid Hormone Are Sensitive to Proteasome Inhibitors in Dispersed Bovine Parathyroid Cells
AM Sakwe, Å Engström, M Larsson, L Rask 		The Journal of biological chemistry 2002
Endocrine System
TC Jones, CC Capen, U Mohr 		1996
Proceedings of the 1982 Laurentian Hormone Conference
L Sokoloff 		Proceedings of the 1982 Laurentian Hormone Conference 1983
The effects of tris(hydroxymethyl)aminomethane and calcium ionophores on the biosynthesis of proparathormone and the formation and degradation of parathormone in bovine parathyroid tissue
DH McGregor, JJ Morrissey, DV Cohn 		The American Journal of Pathology 1981
Oxyphil cell adenoma as a cause of hyperparathyroidism
SH Jone, P Dietler 		The American Journal of Surgery 1981
Klinische Osteologie · A
BA Ashton, HF DeLuca, HJ Dulce, IM Evans, PP Fietzek, HM Frost, LS Galante, JG Ghazarian, HG Haas, JF Habener, F Heuck, CJ Hillyard, H Höhling, KH Knese, J Knop, T Lauffenburger, C Lentner, I MacIntyre, R Montz, BE Nordin, AJ Olah, JA Parsons, G Pfleiderer, JT Potts, WG Robertson, RK Schenk, K Vanselow, K zum Winkel, JM Zanelli, F Kuhlencordt, H Bartelheimer 		1980
Direct release of parathyroid hormone fragments from functioning bovine parathyroid glands in vitro
DA Hanley, K Takatsuki, JM Sultan, AB Schneider, LM Sherwood 		Journal of Clinical Investigation 1978
Functioning oxyphil adenoma of parathyroid gland. An ultrastructural and biochemical study
DH McGregor, LG Lotuaco, MS Rao, LL Chu 		The American Journal of Pathology 1978
Stimulation of Gastrin Secretion and Synthesis in Antral Organ Culture
RF Harty, JC van der Vijver, JE McGuigan 		Journal of Clinical Investigation 1977
Disruption of the Golgi zone and inhibition of the conversion of proparathyroid hormone to parathyroid hormone in human parathyroid tissue by tris(hydroxymethyl)aminomethane
DH McGregor, LL Chu, RR MacGregor, DV Cohn 		The American Journal of Pathology 1977
Axonal transport and axonal processing of low molecular weight proteins from the abdominal ganglion ofAplysia
RW Berry, AW Schwartz 		Brain Research 1977
Metabolic Bone Disease
OL Bijvoet 		Metabolic Bone Disease 1977
CHEMISTRY AND PHYSIOLOGY OF PARATHYROID HORMONE
JO POTTS 		Clinical Endocrinology 1976
Recent advances in calcium metabolism
AW Root, HE Harrison 		The Journal of Pediatrics 1976
Inborn Errors of Calcium and Bone Metabolism
H Bickel, J Stern 		1976
Peptide Hormones
JA Parsons 		1976
Pre - proparathyroid hormone identified by cell - free translation of messenger RNA from hyperplastic human parathyroid tissue
JF Habener, B Kemper, JT Potts, A Rich 		Journal of Clinical Investigation 1975
Regulation of circulating parathyroid hormone levels: Normal physiology and consequences in disorders of mineral metabolism
JA Fischer, JW Blum, W Hunziker, U Binswanger 		Klinische Wochenschrift 1975
The NH2-terminal amino acid sequence of human proparathyroid hormone by radioisotope microanalysis
WY Huang, LL Chu, JW Hamilton, DH McGregor, DV Cohn 		Archives of Biochemistry and Biophysics 1975
Porcine proparathyroid hormone. Identification, biosynthesis, and partial amino acid sequence
LL Chu, WY Huang, ET Littledike, JW Hamilton, DV Cohn 		Biochemistry 1975
Reinvestigation of the amino-terminal sequence of human parathyroid hormone
HT Keutmann, HD Niall, JL O'Riordan, JT Potts 		Biochemistry 1975
Calcium Metabolism, Bone and Metabolic Bone Diseases
F Kuhlencordt, HP Kruse 		1975
Human Parathyroid Hormone IMMUNOLOGICAL CHARACTERIZATION OF ANTIBODIES AGAINST A GLANDULAR EXTRACT AND THE SYNTHETIC AMINO-TERMINAL FRAGMENTS 1-12 AND 1-34 AND THEIR USE IN THE DETERMINATION OF IMMUNOREACTIVE HORMONE IN HUMAN SERA
JA Fischer, U Binswanger, FM Dietrich 		Journal of Clinical Investigation 1974
The N-terminal amino-acid sequence of bovine proparathyroid hormone
JW Hamilton, HD Niall, JW Jacobs, HT Keutmann, JT Potts, DV Cohn 		Proceedings of the National Academy of Sciences 1974
Calcium homeostasis and the pathogenesis and management of hypercalcemic disorders
AB Schneider, LM Sherwood 		Metabolism 1974
Biosynthesis of proparathyroid hormone and parathyroid hormone
DV Cohn, RR MacGregor, LL Chu, DW Huang, CS Anast, JW Hamilton 		The American Journal of Medicine 1974
Radioimmunoassay for Proparathyroid Hormone
JF Habener, GW Tregear, TD Stevens, PC Dee, JT Potts 		Endocrine Research 1974

Advertisement