Citations to this article

Studies on the Nature of Antihemophilic Factor (Factor VIII): FURTHER EVIDENCE RELATING THE AHF-LIKE ANTIGENS IN NORMAL AND HEMOPHILIC PLASMAS
Bruce Bennett, … , Walter B. Forman, Oscar D. Ratnoff
Bruce Bennett, … , Walter B. Forman, Oscar D. Ratnoff
Published September 1, 1973
Citation Information: J Clin Invest. 1973;52(9):2191-2197. https://doi.org/10.1172/JCI107404.
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Studies on the Nature of Antihemophilic Factor (Factor VIII): FURTHER EVIDENCE RELATING THE AHF-LIKE ANTIGENS IN NORMAL AND HEMOPHILIC PLASMAS

Abstract

Normal human antihemophilic factor (AHF, factor VIII) and the protein antigenically related to it in hemophilic plasma both appeared in the void volume of columns of agarose (Sepharose 4B) during purification of these agents. On ultracentrifugation upon sucrose gradients, both agents had sedimentation characteristics similar to those of an S30 marker. After reduction, the polypeptide chains of purified normal AHF and of the nonfunctional agent from hemophilic patients had an apparent molecular weight of 200,000 as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis. These observations suggest that AHF, purified as described, exists as a large molecule with subunits of molecular weight of approximately 200,000.

Authors

Bruce Bennett, Walter B. Forman, Oscar D. Ratnoff

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Year: 2014 2011 1984 1982 1979 1977 1976 1975 1974 1960 Total
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Citations to this article (26)

Title and authors Publication Year
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NA Booth, B Bennett 		British Journal of Haematology 1982
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DE Austen 		Clinics in Haematology 1979
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Comparison of precipitating antisera against normal and abnormal factor IX
KH Ørstavik 		Thrombosis Research 1977
Coagulopathies
JH Lewis, JA Spero, U Hasiba 		Disease-a-Month 1977
The Year in Hematology
AS Gordon, R Silber, J LoBue 		1977
Studies on human antihemophilic factor. Evidence for a covalently linked subunit structure
ME Switzer, PA McKee 		Journal of Clinical Investigation 1976
Factor VIII and Factor-VIII-Related Antigen in Multiple Myelomatosis and Related Conditions
ED Gomperts, G Shulman, SR Lynch 		British Journal of Haematology 1976
In vivo dissociation of factor vii (ahf) activity and factor viii-related antigen in von willebrand's disease
EC Lian, D Deykin 		American Journal of Hematology 1976
Molecular weights of antihaemophilic factor and von Willebrand factor proteins in human plasma
J Newman, RB Harris, AJ Johnson 		Nature 1976
MOLECULAR DEFECTS IN HÆMOPHILIA A AND VON WILLEBRAND'S DISEASE
HR Gralnick, BS Coller 		The Lancet 1976
Carbohydrate Deficiency of the Factor VIII/von Willebrand Factor Protein in von Willebrand's Disease Variants
HR Gralnick, BS Coller, Y Sultan 		Science 1976
Subunit structure of factor VIII antigen synthesized by cultured human endothelial cells
EA Jaffe, RL Nachman 		Journal of Clinical Investigation 1975
Studies of the human factor VIII/von Willebrand factor protein. III. Qualitative defects in von Willebrand's disease
HR Gralnick, BS Coller, Y Sultan 		Journal of Clinical Investigation 1975
ISOLATION, SUBUNIT STRUCTURE, AND PROTEOLYTIC MODIFICATION OF BOVINE FACTOR VIII
ME Legaz, MJ Weinstein, CM Heldebrant, EW Davie 		Annals of the New York Academy of Sciences 1975
HEMOPHILIA A AND VON WILLEBRAND'S DISEASE: THE RELATIONSHIP EXAMINED
B Bennett 		Annals of the New York Academy of Sciences 1975
MOLECULAR STRUCTURAL STUDIES OF HUMAN FACTOR VIII
PA MKee, JC Andersen, ME Switzer 		Annals of the New York Academy of Sciences 1975
THEORETICAL APPROACH TO MOLECULAR BIOLOGY OF FACTOR VIII: HETEROGENEITY OF THE MOLECULE
HR Gralnick, SL Marchesi, BS Coller 		Annals of the New York Academy of Sciences 1975
Cyanogen bromide and tryptic fragments of normal and haemophilic factor VIII
L Holmberg, JO Jeppsson, IM Nilsson, J Stenflo 		Thrombosis Research 1975
The Defect in Hemophilic and von Willebrand's Disease Plasmas Studied by a Recombination Technique
HA Cooper, RH Wagner 		Journal of Clinical Investigation 1974
Annotation
B Bennett 		British Journal of Haematology 1974
Brief Reviews: Some Recent Advances in the Study of Hemostasis
OD Ratnoff 		Circulation research 1974
The Plasma Proteins
ML Petermann 		The Plasma Proteins 1960

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