We have identified two populations of human lymphocytes differing in responsiveness to the plant mitogen concanavalin A (Con-A). When peripheral blood lymphocytes are passed through a nylon column a population of lymphocytes highly responsive to Con-A adheres to the fibers while a second population of cells relatively unresponsive to Con-A emerges from the column. The untreated peripheral blood lymphocytes are termed “unfiltered” cells while the lymphocytes which pass through the column are termed “filtered” cells.
David Boldt, Sister Ann Marie Skinner, Stuart Kornfeld
Title and authors | Publication | Year |
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Discovery and Identification of an Endogenous Metabolite of Tramiprosate and Its Prodrug ALZ-801 that Inhibits Beta Amyloid Oligomer Formation in the Human Brain
JA Hey, P Kocis, J Hort, S Abushakra, A Power, M Vyhnálek, JY Yu, M Tolar |
CNS Drugs | 2018 |
Advances in Immunology
E Pinaud, M Marquet, R Fiancette, S Péron, C Vincent-Fabert, Y Denizot, M Cogné |
Advances in Immunology Volume 110 | 2011 |
Characterization of lectin isolated from Momordica charantia seed as a B cell activator
L Huang, T Adachi, Y Shimizu, Y Goto, J Toyama, H Tanaka, R Akashi, A Sawaguchi, H Iwata, T Haga |
Immunology Letters | 2008 |
Transcriptional regulation of the tartrate-resistant acid phosphatase (TRAP) gene by iron
O Alcantara, SV Reddy, GD Roodman, DH Boldt |
Biochemical Journal | 1994 |
Disparity between expression of transferrin receptor ligand binding and non-ligand binding domains on human lymphocytes
DH Boldt, JL Phillips, O Alcantara |
Journal of Cellular Physiology | 1987 |
Separation of human lymphocyte subclasses by rosettes with latex-lectin particles
ID Dios, M Manso, V Leon, AL Borrasca |
Biochemical Medicine and Metabolic Biology | 1986 |
Biochemical Actions of Hormones
D Marver |
Biochemical Actions of Hormones | 1985 |
Receptors for pea and lentil lectins on human lymphoid cells: Demonstration of distinct lectin-defined cell subclasses
DH Boldt |
Journal of Cellular Physiology | 1984 |
CELLULAR IMMUNOLOGY IN THE BABOON (PAPIO CYNOCEPHALUS): EXAMINATION OF STRUCTURAL AND FUNCTIONAL CHARACTERISTICS OF ADULT AND FETAL LYMPHOID CELLS
DH Boldt, M Fischbach, TJ Kuehl |
European Journal of Immunogenetics | 1984 |
Lymphocyte subpopulations in chronic lymphocytic leukemia detected by lectin binding and flow cytometry
DH Boldt, MO Nelson |
Cancer | 1983 |
Catechol-O-methyltransferase activity and aminergic binding sites distribution in human peripheral blood lymphocyte subpopulations
JM Bidart, P Motte, M Assicot, C Bohuon, D Bellet |
Clinical Immunology and Immunopathology | 1983 |
Blood T-Lymphocyte Functions in Healthy Adults in Relation to Age
C Wedelin, M Björkholm, G Holm, S Ogenstad, H Mellstedt |
Scandinavian Journal of Haematology | 1982 |
Mitogen- and alloantigen-stimulated blastogenic responses of dinitrophenyl-modified human lymphocytes
RF Barth, JJ O'Hara, RJ Duquesnoy, SN Chen, JL Winklehake |
Immunology | 1982 |
Chronic lymphocytic leukemia: Demonstration of cell surface structural heterogeneity by lectin affinity chromatography of cell extracts
DH Boldt |
Leukemia Research | 1982 |
Hyporesponsiveness of Canine Bronchoalveolar Lymphocytes to Mitogens: II.: Analysis of Macromolecular Synthesis, T-Cell Antigen, and Mitogen-Binding
MJ Ansfield, HB Kaltreider |
Experimental Lung Research | 1981 |
Surface Alterations of Mammalian Cells upon Ionizing Radiation as Detected by a Lectin-binding Technique: 1. Binding of Concanavalin A by Blood Cells of X-irradiated Mice
T Kubasova, LP Varga, GJ Köteles |
International Journal of Radiation Biology and Related Studies in Physics, Chemistry and Medicine | 1981 |
Interaction of wheat germ agglutinin with human peripheral blood mononuclear cells. Binding kinetics and flow microfluorometric analysis
DH Boldt |
Molecular Immunology | 1980 |
Immunobiology of primary intracranial tumours. III. Demonstration of a qualitative lymphocyte abnormality in patients with primary brain tumours
TL Roszman, WH Brooks |
Clinical & Experimental Immunology | 1980 |
Fractionation of human lymphocytes with plant lectins. III. Identification of cells regulating the in vitro response to L-phytohaemagglutinin
DH Boldt, RD Lyons |
Immunology | 1980 |
Redistribution of concanavalin a receptors on peripheral blood lymphocytes of myeloma patients
O Wetter, H Delbrück, KH Linder, D Brandhorst |
Leukemia Research | 1980 |
Release of arachidonic acid from human lymphocytes in response to mitogenic lectins
CW Parker, JP Kelly, SF Falkenhein, MG Huber |
Journal of Experimental Medicine | 1979 |
Suppressive effect of alcoholic liver disease sera on lymphocyte transformation
GP Young, FJ Dudley, MB van der Weyden |
Gut | 1979 |
Altered regulation of mitogen responsiveness by suppressor cells in multiple sclerosis
RL Gonzalez, PC Dau, LE Spitler |
Clinical & Experimental Immunology | 1979 |
Fractionation of human lymphocytes with plant lectins
DH Boldt, RD Lyons |
Cellular Immunology | 1979 |
The Antigens
PJ Lachmann |
The Antigens | 1979 |
Cell surface changes in transformed human lymphocytes
SF Speckart, DH Boldt, KL Ryerson |
Experimental Cell Research | 1978 |
Role of adherent cells in immune responses to phytohemagglutinin and concanavalin A
MP Arala-Chaves, L Hope, JH Korn, H Fudenberg |
European Journal of Immunology | 1978 |
Cytochalasin binding in lymphocytes and polymorphonuclear leukocytes
CW Parker, WC Greene, HH MacDonald |
Experimental Cell Research | 1976 |
Kinetics of binding of the toxic lectins abrin and ricin to surface receptors of human cells
K Sandvig, S Olsnes, A Pihl |
The Journal of biological chemistry | 1976 |
The lack of mitogenic response of neuraminidase-treated and untreated human blood lymphocytes to divalent, hexavalent, or insoluble Helix pomatia a hemagglutinin
ML Dillner, S Hammarström, P Perlmann |
Experimental Cell Research | 1975 |
Effect of prednisolone on the leukocyte counts of ponies and on the reactivity of lymphocytes in vitro and in vivo
SP Targowski |
Infection and immunity | 1975 |
Activation of human peripheral blood lymphocytes by concanavalin A dependence of monocytes
E Hedfors, G Holm, D Pettersson |
Clinical & Experimental Immunology | 1975 |
The Effect of In Vivo Hydrocortisone on Subpopulations of Human Lymphocytes
AS Fauci, DC Dale |
Journal of Clinical Investigation | 1974 |
Studies on the interaction between mitogens and human lymphocytes in vitro
VT Skoog, TH Weber, W Richter |
Experimental Cell Research | 1974 |
Characterization of lymphocyte transformation induced by zinc ions
NA Berger, AM Skinner |
The Journal of Cell Biology | 1974 |
Chronic lymphocytic leukaemia. Studies on mitogen-stimulated lymphocyte interferon as a new technique for assessing T lymphocyte effector function
L Epstein, MJ Cline |
Clinical & Experimental Immunology | 1974 |
B cell and T cell lymphopenia in systemic lupus erythematosus
MA Scheinberg, ES Cathcart |
Cellular Immunology | 1974 |
Cell-surface immunoglobulins in chronic lymphocytic leukemia and allied disorders
AC Aisenberg, KJ Bloch, JC Long |
The American Journal of Medicine | 1973 |
Changes in the binding of concanavalin A and wheat germ agglutinin to human lymphocytes during transformation
U Krug, MD Hollenberg, P Cuatrecasas |
Biochemical and Biophysical Research Communications | 1973 |
Technical methods. A simple method for the isolation of blood platelets
P Ganguly, WJ Sonnichsen |
Journal of clinical pathology | 1973 |