Carbonic Anhydrase Function and the Epithelial Organization of H⁺ Secretion in Turtle Urinary Bladder

• Text
• PDF

Abstract

The function of carbonic anhydrase in H+ secretion by the turtle bladder was studied in vitro. Dose response curves were obtained for the carbonic anhydrase inhibitors, acetazolamide and ethoxzolamide, with and without addition of CO2 to the system. In addition, carbonic anhydrase was assayed in homogenates of mucosal cells. The activity in the homogenates was 155±16 U/g dry wt, of which only 11 U represented contamination from erythrocytes; after addition of 5 × 10−6m acetazolamide, no enzyme activity was detectable.

Authors

John H. Schwartz, Seymour Rosen, Philip R. Steinmetz