Studies on the purification and characterization of human factor VIII

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Abstract

Factor VIII (antihemophilic globulin) has been prepared from Hyland method IV AHG and cryoprecipitate using limited chymotryptic digestion followed by Sepharose gel filtration. The activity of factor VIII is unaffected by the digestion procedure, while fibrinogen in converted to large noncoagulable fragments.

Authors

Sally L. Marchesi, N. Raphael Shulman, Harvey R. Gralnick