Cyclic 3′,5′-adenosine monophosphate in the human leukocyte: synthesis, degradation, and effects on neutrophil candidacidal activity

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Abstract

Prostaglandins E1 and E2 (PGE1 and PGE2) stimulate adenyl cyclase activity in broken cell preparations of normal human leukocytes, whereas prostaglandin F1a produces no effect. PGE1 and PGE2 also cause increased accumulation of cyclic 3′,5′-adenosine monophosphate-3H (3H-labeled AMP) in intact leukocytes which have been preincubated with adenine-3H in vitro. Theophylline inhibits leukocyte phosphodiesterase activity and potentiates the stimulatory effect of the prostaglandins on intracellular accumulation of cyclic 3′,5′-AMP-3H.

Authors

Henry R. Bourne, Robert I. Lehrer, Martin J. Cline, Kenneth L. Melmon