Citations to this article

C1r, subunit of the first complement component: purification, properties, and assay based on its linking role
Maria M. E. De Bracco, R. M. Stroud
Maria M. E. De Bracco, R. M. Stroud
Published April 1, 1971
Citation Information: J Clin Invest. 1971;50(4):838-848. https://doi.org/10.1172/JCI106555.
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Research Article

C1r, subunit of the first complement component: purification, properties, and assay based on its linking role

Abstract

A method to obtain C1r, a subunit of the first complement component, in a highly purified state has been described for the first time. The stepwise method starts with a neutral euglobulin precipitation, after diethylaminoethyl- and carboxymethyl-cellulose chromatography and a final preparative polyacrylamide electrophoresis step. Such C1r preparations are devoid of C1q and C1s activities and show only one protein band on analytic polyacrylamide electrophoresis. Rabbits injected with this preparation produced antisera showing only one precipitation band. The stability of C1r activity was determined under different conditions, and C1r was found to be labile at 37°C, pH 7-8 and low ionic strength.

Authors

Maria M. E. De Bracco, R. M. Stroud

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Citations to this article (39)

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Advances in Protein Chemistry
CM Silva, J Isgaard, MO Thorner 		Advances in Protein Chemistry 1998
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GR Stuart, NJ Lynch, J Lu, A Geick, BE Moffatt, RB Sim, WJ Schwaeble 		FEBS Letters 1996
The Complement System
A Agostoni, M Cicardi, M Gardinali, L Bergamaschini 		International journal of immunopathology and pharmacology 1992
A novel leupeptin-sensitive serine endopeptidase present in normal and malignant rat mammary tissues
I Eto, MD Bandy 		Molecular and Cellular Biochemistry 1990
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T Sasaki, K Yonemasu, M Matsumoto, K Nagaki 		Microbiology and Immunology 1989
Complete amino acid sequence of the catalytic chain of human complement subcomponent C.hivin.1r
GJ Arlaud, J Gagnon 		Biochemistry 1983
Characterization of the activation of the human C1r complement molecule
J Bauer, G Valet 		Molecular Immunology 1982
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GJ Arlaud, J Gagnon, RR Porter 		Biochemical Journal 1982
Fluid phase activation of proenzymic C1r purified by affinity chromatography
CL Villiers, AM Duplaa, GJ Arlaud, MG Colomb 		Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology 1982
Isolierung und Kristallisation der Human-Komplement-Subkomponente Clr̄ des Cl-Komplexes
H HAUPT, S BAUNDER 		Hoppe-Seyler´s Zeitschrift für physiologische Chemie 1981
Familial C1q deficiency associated with renal and cutaneous disease
F Leyva-Cobián, I Moneo, F Mampaso, M Sánchez-Bayle, JL Ecija, A Bootello 		Clinical & Experimental Immunology 1981
C $$\bar l$$ r and C $$\bar l$$ s subcomponents of human complement: Two serine proteinases lacking the ?histidine-loop? disulphide bridge
GJ Arlaud, J Gagnon 		Bioscience Reports 1981
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DH Bing, JM Andrews, KM Morris, E Cole, V Irish 		Preparative Biochemistry 1980
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MM de Bracco, MT Rimoldi, PM Cossio, A Rabinovich, JI Maiztegui, G Carballal, RM Arana 		New England Journal of Medicine 1978
Direct Demonstration and Quantitation of the First Complement Component in Human Serum
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Complement: A host defense mechanism ready for pharmacological manipulation?
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RB Sim, RR Porter, KB Reid, I Gigli 		Biochemical Journal 1977
Studies on the precursor from of the first component of complement—I
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IMMUNOCHEMISTRY AND IMMUNOBIOLOGY OF THE COMPLEMENT SYSTEM
I Gigli 		Journal of Investigative Dermatology 1976
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AB Laurell, U Mártensson, AG Sjöholm 		Acta Pathologica Microbiologica Scandinavica Section C Immunology 1976
Ultracentrifugation studies on the native form of the first component of human complement (C1)
M Loos, HU Hill, B Wellek, HP Heinz 		FEBS Letters 1976
Partial purification and autocatalytic activation of the subunit of the first component of human complement, C1r
K Takahashi, S Nagasawa, J Koyama 		FEBS Letters 1976
Proteolysis and Physiological Regulation
NR Cooper, RJ Ziccardi 		Proteolysis and Physiological Regulation 1976
The human complement system
JE Volanakis 		Journal of Oral Pathology and Medicine 1975
A gross structure of an activated form of a subunit of the first component of human complement. Cl
K Takahashi, S Nagasawa, J Koyama 		FEBS Letters 1975
Modern Aspects of Electrochemistry: Volume 18
RE White, JO Bockris, BE Conway 		1975
Isolation of a complex of the subcomponents of the activated first component of complement, ClrCls, from ACD-Human plasma
S Nagasawa, K Takahashi, J Koyama 		FEBS Letters 1974
Specific antisera to Clr. Quantitation of Clr in normal and pathological human sera
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The activation of Cls with purified Clr
K Sakai, RM Stroud 		Immunochemistry 1974
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G Zeipel, A Sjöholm, AB Laurell 		Acta Pathologica Microbiologica Scandinavica Section B Microbiology and Immunology 1973
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C1r deficiency: an inborn error associated with cutaneous and renal disease
NK Day, H Geiger, R Stroud, M DeBracco, B Mancaido, D Windhorst, RA Good 		Journal of Clinical Investigation 1972
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Progress in Immunology
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The Plasma Proteins
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