The effect of phytohemagglutinin (PHA) on the ability of human lymphocytes to transport the nonutilizable amino acid, α-aminoisobutyric acid (AIB) has been studied. PHA binds rapidly to plasma membrane receptor sites with half maximal binding requiring approximately 7.5 min. During the first 30 min after PHA addition to lymphocytes no change was detected in AIB transport, but then a linear increase in the initial rate of AIB transport occurred over the next 9 hr. Subsequently, the rate of AIB transport stabilized at a level 6-7 times greater than that found in control lymphocytes. The change in membrane function developed even when de novo protein synthesis was inhibited by 85-90% with puromycin or cycloheximide. However, the PHA effect did not occur when the lymphocytes were maintained at 4°C.
John Mendelsohn, Sister Ann Skinner, Stuart Kornfeld
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Cellular Immunology | 1978 |
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Cellular Immunology | 1978 |
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Contact-Mediated Bone Resorption by Human Monocytes in Vitro
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Exploring the action and specificity of cobra venom phospholipase A2 toward human erythrocytes, ghost membranes, and lipid mixtures
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Journal of Allergy and Clinical Immunology | 1977 |
Endocytosis and exocytosis of phytohemagglutinin (PHA) cell surface receptors of human lymphocytes during blast transformation
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Clinical & Experimental Immunology | 1977 |
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Clinical & Experimental Immunology | 1977 |
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Immune response in humans after vaccination with vaccinia virus: generation of a virus-specific cytotoxic activity by human peripheral lymphocytes
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Clinical & Experimental Immunology | 1977 |
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Biochimica et Biophysica Acta (BBA) - General Subjects | 1977 |
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Biochimica et Biophysica Acta (BBA) - General Subjects | 1977 |
The Diversity of Membrane
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The Diversity of Membrane | 1977 |
Purine Metabolism in Man—II
MM Müller, E Kaiser, JE Seegmiller |
1977 | |
Mechanisms of lymphocyte activation. Binding kinetics of phytohemagglutinin to human lymphocytes
GB Faguet |
The Journal of biological chemistry | 1977 |
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Journal of Clinical Investigation | 1976 |
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Journal of Clinical Investigation | 1976 |
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Journal of Clinical Investigation | 1976 |
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Experimental Cell Research | 1976 |
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Experimental Cell Research | 1976 |
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Biochimica et Biophysica Acta (BBA) - Biomembranes | 1976 |
Phospholipid metabolism of stimulated lymphocytes
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Biochimica et Biophysica Acta (BBA) - Biomembranes | 1976 |
Etude cinétique de l'activité ATPase activée par Ca2+ ou Mg2+ des membranes plasmiques lymphocytaires
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Journal of Cellular Physiology | 1976 |
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Immunological Investigations | 1976 |
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Proceedings of the National Academy of Sciences | 1976 |
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Journal of Experimental Medicine | 1976 |
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Biochimica et Biophysica Acta (BBA) - General Subjects | 1976 |
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Archives of Biochemistry and Biophysics | 1976 |
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Cellular Immunology | 1976 |
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Cellular Immunology | 1976 |
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Immunochemistry | 1976 |
The Reticuloendothelial System in Health and Disease
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1976 | |
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P Cuatrecasas, MF Greaves |
1976 | |
Opposing effects of mitogenic and nonmitogenic lectins on lymphocyte activation. Evidence that wheat germ agglutinin produces a negative signal
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The Journal of biological chemistry | 1976 |
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DA Lawrence, WO Weigle, HL Spiegelberg |
Journal of Clinical Investigation | 1975 |
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MR Quastel, JG Kaplan |
Experimental Cell Research | 1975 |
Ortho-phenanthroline inhibition of DNA synthesis in mammalian cells
NA Berger, ES Johnson, SA Skinner |
Experimental Cell Research | 1975 |
Nascent DNA from Phytohemagglutinin-Stimulated Human Lymphocytes
J Mendelsohn, EM Fox, M Goulian, E Barbosa |
Immunological Investigations | 1975 |
Propriétés d'une ATPase Ca2+ ou Mg2+ dépendante des membranes plasmiques de lymphocytes
J Dornand, JC Mani, M Mousseron-Canet, B Pau |
Biochimie | 1975 |
A rapid phytohemagglutinin induced alteration in lymphocyte potassium permeability
GB Segel, MM Hollander, BR Gordon, MR Klemperer, MA Lichtman |
Journal of Cellular Physiology | 1975 |
Increased sensitivity of lymphocyte Δ1pyrroline-5-carboxylate reductase to inhibition by proline with transformation
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Nature | 1975 |
Effects of mitogens on sodium-potassium transport, 3H-ouabain binding, and adenosine triphosphatase activity in lymphocytes
R Averdunk, PK Lauf |
Experimental Cell Research | 1975 |
Fetuin, an inhibitor of lymphocyte transformation. The interaction of fetuin with phytomitogens and a possible role for fetuin in fetal development
S Yachnin |
Journal of Experimental Medicine | 1975 |
An absolute requirement for serum macromolecules in phytohaemagglutinin-induced human lymphocyte DNA synthesis
S Yachnin, J Raymond |
Clinical & Experimental Immunology | 1975 |
Detection and ultrastructural localization of human smooth muscle myosin-like molecules in human non-muscle cells by specific antibodies
RG Painter, M Sheetz, SJ Singer |
Proceedings of the National Academy of Sciences | 1975 |
Multiple protein kinases from human lymphocytes. Identification enzymes phosphorylating exogenous histon and casein
BE Kemp, M Froscio, A Rogers, AW Murray |
Biochemical Journal | 1975 |
Pathogenesis of of cytomegalovirus infection. I. Activation of virus from bone marrow-derived lymphocytes by in vitro allogenic reaction
LB Olding, FC Jensen, MB Oldstone |
Journal of Experimental Medicine | 1975 |
The oncornavirus glycoprotein gp69/71: a constituent of the surface of normal and malignant thymocytes
BC Vellano, B Nave, BP Croker, RA Lerner, FJ Dixon |
Journal of Experimental Medicine | 1975 |
Non specific effector-induced enzyme modulation in isolated plasma membranes
A Zachowski, D Migliore-Samour, A Paraf, P Jollès |
FEBS Letters | 1975 |
Molecular Approaches to Immunology
RW Holley, IS Trowbridge |
Molecular Approaches to Immunology | 1975 |
Immune Recognition
HJ Wedner, FE Bloom, CW Parker |
Immune Recognition | 1975 |
Einundachtzigster Kongress
B Schlegel |
1975 | |
Accelerated active transport of alpha aminoisobutyric acid by human leukemic leukocytes in an amino acid deficient environment
PA Frengley, WA Peck, MA Lichtman |
Experimental Cell Research | 1974 |
Regulation of amino acid uptake in lymphocytes stimulated by mitogens
KJ Berg, I Betel |
Experimental Cell Research | 1974 |
Concanavalin A-induced alterations in sodium and potassium content of Ehrlich ascites tumor cells
F Aull, MS Nachbar |
Journal of Cellular Physiology | 1974 |
Some biological characteristics of a pituitary growth factor (CGF) for cultured lapine articular chondrocytes
CJ Malemud, L Sokoloff |
Journal of Cellular Physiology | 1974 |
Lymphocytes from human newborns abrogate mitosis of their mother's lymphocytes
LB Olding, MB Oldstone |
Nature | 1974 |
Characterization of lymphocyte transformation induced by zinc ions
NA Berger, AM Skinner |
The Journal of Cell Biology | 1974 |
Impaired Lymphocyte Function in Untreated Hodgkin's Disease
R Levy, HS Kaplan |
New England Journal of Medicine | 1974 |
Correlation between mitogenicity and stimulation of calcium uptake in human lymphocytes
CW Parker |
Biochemical and Biophysical Research Communications | 1974 |
The effect of specific glycosidase treatment on the responsiveness of lymphocytes
F Miller, PS Chase |
Cellular Immunology | 1974 |
Absence of an early increase of phospholipid-phosphate turnover in mitogen-stimulated B lymphocytes
I Betel, J Martijnse, KJ van Berg |
Cellular Immunology | 1974 |
Correlation of early changes in amino acid transport and DNA synthesis in stimulated lymphocytes
KJ Berg, I Betel |
Cellular Immunology | 1974 |
Evidence for translational control of “early” protein synthesis in lymphocytes stimulated with concanavaline A
RE Wettenhall, DR London |
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis | 1974 |
Clinical Immunobiology
LE Spitler, AS Levin, HH Fudenberg |
Clinical Immunobiology | 1974 |
Concanavalin A augments the turnover of electrophoretically defined thymocyte plasma membrane proteins
R Schmidt-Ullrich, DF Wallach, E Ferber |
Biochimica et Biophysica Acta (BBA) - Biomembranes | 1974 |
Alterations in Cyclic Adenosine Monophosphate Metabolism in Human Bronchial Asthma. I. LEUKOCYTE RESPONSIVENESS TO β-ADRENERGIC AGENTS
CW Parker, JW Smith |
Journal of Clinical Investigation | 1973 |
Enhanced Effects of Prostaglandin E1 and Dibutyryl Cyclic AMP upon Human Lymphocytes in the Presence of Cortisol
J Mendelsohn, MM Multer, RF Boone |
Journal of Clinical Investigation | 1973 |
Effects of chelating agents on the initial interaction of phytohemagglutinin with lymphocytes and the subsequent stimulation of amino acid uptake
RB Whitney, RM Sutherland |
Biochimica et Biophysica Acta (BBA) - Biomembranes | 1973 |
Characteristics of calcium accumulation by lymphocytes and alterations in the process induced by phytohemagglutinin
RB Whitney, RM Sutherland |
Journal of Cellular Physiology | 1973 |
Increased transport of 2-aminoisobutyric acid in rat lymphocytes stimulated with concanavalin A
KJ Berg, I Betel |
Experimental Cell Research | 1973 |
Effect of phytohaemagglutinin on the carbohydrate metabolism of human blood lymphocytes after inhibition of the oxidative phosphorylation
D Roos, JA Loos |
Experimental Cell Research | 1973 |
Selective early activation of a sodium dependent amino acid transport system in stimulated rat lymphocytes
KJ Berg, I Betel |
FEBS Letters | 1973 |
Properties of an oncornavirus glycoprotein: Evidence for its presence on the surface of virions and infected cells
SJ Kennel, BC Villano, RL Levy, RA Lerner |
Virology | 1973 |
The control of protein synthesis during the stimulation of lymphocytes by phytohaemagglutinin
T Ahern, JE Kay |
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis | 1973 |
Proteins synthesized in lymphoid cells early after activation by phytohemagglutinin
R Levy, SA Rosenberg |
Cellular Immunology | 1973 |
Drugs and the Cell Cycle
HL Cooper |
Drugs and the Cell Cycle | 1973 |
Contemporary Topics in Molecular Immunology
RA Reisfeld, WJ Mandy |
1973 | |
Alpha-aminoisobutyric acid transport in human leukemic lymphocytes: in vitro characteristics and inhibition by cortisol and cycloheximide
DT Baran, MA Lichtman, WA Peck |
Journal of Clinical Investigation | 1972 |
Lymphocyte Transformation Induced by Autologous Cells: Stimulation by Cultured Lymphoblast Lines
ME Weksler, G Birnbasum |
Journal of Clinical Investigation | 1972 |
Studies of Two Subpopulations of Human Lymphocytes Differing in Responsiveness to Concanavalin A
S Boldt, AM Skinner, S Kornfeld |
Journal of Clinical Investigation | 1972 |
Requirement for calcium ions in lymphocyte transformation stimulated by phytohemagglutinin
RB Whitney, RM Sutherland |
Journal of Cellular Physiology | 1972 |
Lymphocyte activation: Rapid changes in the phospholipid metabolism of plasma membranes during stimulation
K Resch, EW Gelfand, K Hansen, E Ferber |
European Journal of Immunology | 1972 |
Dose-response of lymphocyte carbohydrate metabolism to phytohaemagglutinin
D Roos, JE Boer, L Huismans, AJ Boom |
Experimental Cell Research | 1972 |
Lymphocyte stimulation by phytohaemagglutinin: Role of the early stimulation of potassium uptake
JE Kay |
Experimental Cell Research | 1972 |
Lymphocyte monovalent cation metabolism: Cell volume, cation content and cation transport
MA Lichtman, AH Jackson, WA Peck |
Journal of Cellular Physiology | 1972 |
Elicitation of Selective T and B Lymphocyte Responses by Cell Surface Binding Ligands
M Greaves, G Janossy |
Immunological Reviews | 1972 |
Phospholipid Metabolism of Stimulated Lymphocytes. Effects of Phytohemagglutinin, Concanavalin A and an Anti-Immunoglobulin Serum
K Resch, E Ferber |
European Journal of Biochemistry | 1972 |
Interaction of Concanavalin A with Rat Lymphocytes
I Betel, KJ BERG |
European Journal of Biochemistry | 1972 |
Microviscosity of the cell membrane
B Rudy, C Gitler |
Biochimica et Biophysica Acta (BBA) - Biomembranes | 1972 |
Histone phosphatase and cyclic nucleotide-stimulated protein kinase from human lymphocytes
AW Murray, M Froscio, BE Kemp |
Biochemical Journal | 1972 |
Control of transcription of RNA rich in polyadenylic acid in human lymphocytes
MG Rosenfeld, JB Abrass, J Mendelsohn, BA Ross, RF Boone, LD Garren |
Proceedings of the National Academy of Sciences | 1972 |
Production of macrophage migration inhibition factor by continuous cell lines
DG Tubergen, JD Feldman, EM Pollock, RA Lerner |
Journal of Experimental Medicine | 1972 |
The induction of ornithine decarboxylase during lymphocyte stimulation by phytohaemagglutinin
JE Kay, VJ Lindsay |
Biochemical Journal | 1972 |
The immunological and physicochemical properties of mitogenic proteins derived from Phaseolus vulgaris
S Yachnin, RH Svenson |
Immunology | 1972 |
Excretion of deoxyribonucleic acid by lymphocytes stimulated with phytohemagglutinin or antigen
JC Rogers, D Boldt, S Kornfeld, A Skinner, CR Valeri |
Proceedings of the National Academy of Sciences | 1972 |
Sugar and Amino Acid Transport by Cells in Culture — Differences between Normal and Malignant Cells
D Deykin, C Balko, KJ Isselbacher |
New England Journal of Medicine | 1972 |
Insulin Action in Isolated Rat Thymocytes
ID Goldfine, P Sherline |
The Journal of biological chemistry | 1972 |
Characterization of the Cell Surface Receptor for the Agaricus bisporus Hemagglutinin
CA Presant, S Kornfeld |
The Journal of biological chemistry | 1972 |
The Nature of the Cell Surface Receptor Site for Lens culinaris Phytohemagglutinin
S Kornfeld, J Rogers, W Gregory |
The Journal of biological chemistry | 1971 |