Citations to this article

Quantitative immunology of immune hemolytic anemia: I. The fixation of C1 by autoimmune antibody and heterologous anti-IgG antibody
Wendell F. Rosse
Wendell F. Rosse
Published April 1, 1971
Citation Information: J Clin Invest. 1971;50(4):727-733. https://doi.org/10.1172/JCI106543.
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Quantitative immunology of immune hemolytic anemia: I. The fixation of C1 by autoimmune antibody and heterologous anti-IgG antibody

Abstract

The fixation of the first component of complement (C[unk]1) by rabbit and goat anti-IgG antibodies reacting with auto- or isoimmune antibodies attached to red cells has been investigated. Two molecules of the rabbit IgG anti-IgG were required to fix a single molecule of C[unk]1, whereas only one molecule of goat IgM anti-IgG was required. The relationship between the number of auto- or isoimmune antibody molecules attached to the red cells and the amount of C[unk]1 fixed by anti-IgG was determined by the concentration of anti-IgG. A concentration of anti-IgG was found such that the number of molecules of C[unk]1 fixed was directly proportional to the concentration of auto- or isoimmune antibody. By this method a sensitive, reproducible minimum estimate of the amount of cell-bound and serum antibody could be made.

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Wendell F. Rosse

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Year: 2024 2017 2014 1983 1975 1973 1971 Total
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Citations to this article (10)

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Abendstein L, Noteborn WE, Veenman LS, Dijkstra DJ, van de Bovenkamp FS, Trouw LA, Sharp TH 		Journal of the American Chemical Society 2024
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TZ Chang, I Diambou, JR Kim, B Wang, JA Champion 		Bioengineering & Translational Medicine 2017
Host- and pathogen-derived adjuvant coatings on protein nanoparticle vaccines: CHANG et al
TZ Chang, I Diambou, JR Kim, B Wang, JA Champion 		Bioengineering & Translational Medicine 2017
Complement Is Activated by IgG Hexamers Assembled at the Cell Surface
CA Diebolder, FJ Beurskens, RN de Jong, RI Koning, K Strumane, MA Lindorfer, M Voorhorst, D Ugurlar, S Rosati, AJ Heck, JG van de Winkel, IA Wilson, AJ Koster, RP Taylor, EO Saphire, DR Burton, J Schuurman, P Gros, PW Parren 		Science 2014
Antibodies reactive with surface membranes of cellular elements in the blood
DB Cines 		Human Pathology 1983
Effect of corticosteroids on the human monocyte IgG and complement receptors
AD Schreiber, J Parsons, P McDermott, RA Cooper 		Journal of Clinical Investigation 1975
Use of horseradish peroxidase-labelled antiglobulin for the colorimetric quantitation of erythrocyte antibodies
TJ Greenwalt, EM Swierk, EA Steane 		Journal of Immunological Methods 1975
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GL Logue, WF Rosse, JP Gockerman 		Journal of Clinical Investigation 1973
Effect of immunoglobulin class and affinity on the initiation of complement-dependent damage to liposomal model membranes sensitized with dinitrophenylated phospholipids
HR Six, K Uemura, SC Kinsky 		Biochemistry 1973
Quantitative immunology of immune hemolytic anemia II. The relationship of cell-bound antibody to hemolysis and the effect of treatment
WF Rosse 		Journal of Clinical Investigation 1971

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