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Citations to this article

Cyclic adenosine 3′,5′-monophosphate in human lymphocytes. Alterations after phytohemagglutinin stimulation
Jay W. Smith, … , W. Marcus Newberry Jr., Charles W. Parker
Jay W. Smith, … , W. Marcus Newberry Jr., Charles W. Parker
Published February 1, 1971
Citation Information: J Clin Invest. 1971;50(2):432-441. https://doi.org/10.1172/JCI106510.
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Cyclic adenosine 3′,5′-monophosphate in human lymphocytes. Alterations after phytohemagglutinin stimulation

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Abstract

We have studied cyclic adenosine 3′,5′-monophosphate (cyclic AMP) concentrations in human peripheral blood lymphocytes after stimulation with phytohemagglutinin (PHA), isoproterenol, prostaglandins, and aminophylline. Purified lymphocytes were obtained by nylon fiber chromatography, and low speed centrifugation to remove platelets. Cyclic AMP levels were determined by a highly sensitive radioimmunoassay. At concentrations of 0.1-1.0 mmoles/liter isoproterenol and aminophylline produced moderate increases in cyclic AMP concentrations, whereas prostaglandins produced marked elevations. High concentrations of PHA produced 25-300% increases in cyclic AMP levels, alterations being demonstrated within 1-2 min. The early changes in cyclic AMP concentration appear to precede previously reported metabolic changes in PHA-stimulated cells. After 6 hr cyclic AMP levels in PHA-stimulated cells had usually fallen to the levels of control cells. After 24 hr the level in PHA-stimulated cells was characteristically below that of the control cells.

Authors

Jay W. Smith, Alton L. Steiner, W. Marcus Newberry Jr., Charles W. Parker

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Lymphocyte and macrophage adenyl cyclase activity in animais with enhanced cell-mediated resistance to infection and tumors
MJ Droller, JS Remington
Cellular Immunology 1975
Induction of T-cell dependent splenic prostaglandin F2α by T-cell dependent antigen
PL Osheroff, DR Webb, J Paulsrud
Biochemical and Biophysical Research Communications 1975
Immune Recognition
HJ Wedner, FE Bloom, CW Parker
Immune Recognition 1975
Methods in Membrane Biology
ED Korn
1975
Human Tumor Cells in Vitro
J Fogh
1975
Phosphorylation of Lymphocyte Nuclear Acidic Proteins: Regulation by Cyclic Nucleotides
EM Johnson, JW Hadden
Science 1975
Release of Prostaglandin by Mitogen- and Antigen-Stimulated Leukocytes in Culture
VA Ferraris, FR DeRubertis
Journal of Clinical Investigation 1974
CELL MIGRATION INHIBITION: AN APPRAISAL
J Morley
Allergy 1974
Biphasic effect of cyclic AMP on an immune response
HS Teh, V Paetkau
Nature 1974
Inhibition by X-irradiation and antimetabolites of DNA synthesis without affecting cAMP elevation in isoproterenol-stimulated mouse parotid gland
I Furuno, K Asami, H Matsudaira
Experimental Cell Research 1974
Soluble lymphocytic mediators. I. Inhibition of macrophage migration inhibitory factor production by drugs
E Pick
Immunology 1974
Les prostaglandines et la réponse immunitaire
LF Perrin
Revue Française d'Allergologie et d'Immunologie Clinique 1974
Cyclic amp, prostaglandins, and the control of cell proliferation
JP MacManus, JF Whitfield
Prostaglandins 1974
Anti-inflammatory Agents
RJ Haslam
Anti-inflammatory Agents 1974
Adenylate cyclase activity in lymphoid organs during regional and systemic graft-versus-host reactions
JN Singh, NS Dhalla
Biochimica et Biophysica Acta (BBA) - Enzymology 1974
Correlation between mitogenicity and stimulation of calcium uptake in human lymphocytes
CW Parker
Biochemical and Biophysical Research Communications 1974
Effects of phytohemagglutinin and concanavalin-A on mammalian epidermal cells
SJ Stegman, ND Bonfilio, K Fukuyama, WL Epstein
Cell Differentiation 1974
Biochemistry of Lipids
E Horton
Biochemistry of Lipids 1974
Concanavalin A augments the turnover of electrophoretically defined thymocyte plasma membrane proteins
R Schmidt-Ullrich, DF Wallach, E Ferber
Biochimica et Biophysica Acta (BBA) - Biomembranes 1974
Immunological Aspects of Allergy and Allergic Diseases
E Rajka, S Korossy
1974
Cyclic adenosine-3?,5?-monophosphate in cellular immunity
AA Hakim
Die Naturwissenschaften 1974
Cyclic AMP, Cell Growth, and the Immune Response
W Braun, LM Lichtenstein, CW Parker
1974
Cyclic Nucleotides and their Role in Gastrointestinal Secretion
DV Kimberg
Gastroenterology 1974
Modulation of Inflammation and Immunity by Cyclic AMP: Receptors for vasoactive hormones and mediators of inflammation regulate many leukocyte functions
HR Bourne, Y Weinstein, KL Melmon, LM Lichtenstein, CS Henney, GM Shearer
Science 1974
Cyclic AMP, Cell Growth, and the Immune Response: Proceedings of the Symposium Held at Marco Island, Florida January 8–10, 1973
W Braun, LM Lichtenstein, CW Parker
1974
Early Nuclear Events in the Induction of Lymphocyte Proliferation by Mitogens
EM Johnson, J Karn, VG Allfrey
The Journal of biological chemistry 1974
Alterations in Cyclic Adenosine Monophosphate Metabolism in Human Bronchial Asthma. I. LEUKOCYTE RESPONSIVENESS TO β-ADRENERGIC AGENTS
CW Parker, JW Smith
Journal of Clinical Investigation 1973
Effects of Cholera Enterotoxin on Adenosine 3′,5′-Monophosphate and Neutrophil Function. COMPARISON WITH OTHER COMPOUNDS WHICH STIMULATE LEUKOCYTE ADENYL CYCLASE
HR Bourne, RI Lehrer, LM Lichtenstein, G Weissmann, R Zurier
Journal of Clinical Investigation 1973
Alterations in Cyclic AMP Metabolism in Human Bronchial Asthma. II. LEUKOCYTE AND LYMPHOCYTE RESPONSES TO PROSTAGLANDINS
CW Parker, ML Baumann, MG Huber
Journal of Clinical Investigation 1973
Alterations in Cyclic AMP Metabolism in Human Bronchial Asthma. II. LEUKOCYTE AND LYMPHOCYTE RESPONSES TO PROSTAGLANDINS
CW Parker, ML Baumann, MG Huber
Journal of Clinical Investigation 1973
Enhanced Effects of Prostaglandin E1 and Dibutyryl Cyclic AMP upon Human Lymphocytes in the Presence of Cortisol
J Mendelsohn, MM Multer, RF Boone
Journal of Clinical Investigation 1973
Calcium, cyclic adenosine 3′, 5′-monophosphate, and the control of cell proliferation: A review
JF Whitfield, RH Rixon, JP MacManus, SD Balk
In Vitro 1973
Effects of chelating agents on the initial interaction of phytohemagglutinin with lymphocytes and the subsequent stimulation of amino acid uptake
RB Whitney, RM Sutherland
Biochimica et Biophysica Acta (BBA) - Biomembranes 1973
The effect of extracellular calcium and magnesium on the proliferation of murine lymphoblasts
JK Brennan, MA Lichtman
Journal of Cellular Physiology 1973
Characteristics of calcium accumulation by lymphocytes and alterations in the process induced by phytohemagglutinin
RB Whitney, RM Sutherland
Journal of Cellular Physiology 1973
EPIDERMAL INTERCELLULAR STAINING WITH FLUORESCEIN-CONJUGATED PHYTOHEMAGGLUTININS
ML Nieland
Journal of Investigative Dermatology 1973
Studies on thymus products. VI. The effects of cyclic nucleotides and prostaglandins on rosette-forming cells. Interactions with thymic factor
MA Bach, JF Bach
European Journal of Immunology 1973
Stimulation of adenylate cyclase activity in lymph nodes of rats with graft-versus-host disease
JN Singh, NS Dhalla
Cellular Immunology 1973
Promotion of replication in lymphoid cells by specific thiols and disulfides in vitro. Effects on mouse lymphoma cells in comparison with splenic lymphocytes
JD Broome, MW Jeng
Journal of Experimental Medicine 1973
The depression of phagocytosis by exogenous cyclic nucleotides, prostaglandins, and theophylline
JP Cox, ML Karnovsky
The Journal of Cell Biology 1973
A cell-contact model for cellular position determination in development
D McMahon
Proceedings of the National Academy of Sciences 1973
The role of adenosine 3',5'-cyclic monophosphate in the regulation of mammalian cell division
CW Abell, TM Monahan
The Journal of Cell Biology 1973
The effect of Bordetella pertussis on lymphocyte cyclic AMP metabolism
CW Parker, SI Morse
Journal of Experimental Medicine 1973
Application of competitive ligand-binding radioassays to the nucleotides
AL Steiner
Metabolism 1973
Mitogen-induced human lymphocyte activation in serum-free medium
DR Webb, DP Stites, J Perlman, HH Fudenberg
Clinical Immunology and Immunopathology 1973
Lymphocyte activation: The dualistic effect of cAMP
DR Webb, DP Stites, JD Perlman, D Luong, HH Fudenberg
Biochemical and Biophysical Research Communications 1973
Cyclic AMP inhibition of cytotoxin (“lymphotoxin”) elaboration by stimulated lymphocytes
RB Lies, JB Peter
Cellular Immunology 1973
Drugs and the Cell Cycle
HL Cooper
Drugs and the Cell Cycle 1973
Contemporary Topics in Molecular Immunology
RA Reisfeld, WJ Mandy
1973
Neunundsiebzigster Kongress
K Miehlke
1973
The Role of Cyclic AMP and Calcium in Cell Activatio
H Rasmussen, DB Goodman, A Tenenhouse, AB Borle
Critical Reviews in Biochemistry and Molecular Biology 1972
In vitro response of lymphocytes to phytohemagglutinin (PHA) as studied with antiserum to PHA
LH Younkin
Experimental Cell Research 1972
Requirement for calcium ions in lymphocyte transformation stimulated by phytohemagglutinin
RB Whitney, RM Sutherland
Journal of Cellular Physiology 1972
Cultured human lymphocytes
GE Moore
Journal of Surgical Oncology 1972
Lymphocyte activation: Rapid changes in the phospholipid metabolism of plasma membranes during stimulation
K Resch, EW Gelfand, K Hansen, E Ferber
European Journal of Immunology 1972
The influence of calcium on the cyclic AMP-mediated stimulation of DNA synthesis and cell proliferation by prostaglandin E1
JF Whitfield, JP Macmanus, BM Braceland, DJ Gillan
Journal of Cellular Physiology 1972
Histone phosphatase and cyclic nucleotide-stimulated protein kinase from human lymphocytes
AW Murray, M Froscio, BE Kemp
Biochemical Journal 1972
Guanosine 3':5'-cyclic monophosphate: a possible intracellular mediator of mitogenic influences in lymphocytes
JW Hadden, EM Hadden, MK Haddox, ND Goldberg
Proceedings of the National Academy of Sciences 1972
Emergence of insulin receptors on human lymphocytes during in vitro transformation
U Krug, F Krug, P Cuatrecasas
Proceedings of the National Academy of Sciences 1972
Control of transcription of RNA rich in polyadenylic acid in human lymphocytes
MG Rosenfeld, JB Abrass, J Mendelsohn, BA Ross, RF Boone, LD Garren
Proceedings of the National Academy of Sciences 1972
Cyclic 3′,5′-AMP phosphodiesterase of human blood lymphocytes
A Lagarde, L Colobert
Biochimica et Biophysica Acta (BBA) - Enzymology 1972
Regulation of Organ and Tissue Growth
B Lytton
Regulation of Organ and Tissue Growth 1972
The Prostaglandins
MF Cuthbert
The Prostaglandins 1972
Enhanced uptake of calcium by transforming lymphocytes
RB Whitney, RM Sutherland
Cellular Immunology 1972
Study of a cyclic AMP-dependent protein kinase from calf thymus
M Pierre, JE Loeb
Biochimica et Biophysica Acta (BBA) - Enzymology 1972
Prostaglandins in Cellular Biology
PW Ramwell, BB Pharriss
1972
Activation of T and B Lymphocytes by Insoluble Phytomitogens
MF Greaves, S Bauminger
Nature: New biology 1972
Contemporary Topics in Immunochemistry
FP Inman
1972
Leukämie
R Gross, J van de Loo
1972
Stimulation of Ribonucleic Acid Synthesis in Horse Lymphocytes by Exogenous Cyclic Adenosine 3′,5′-Monophosphate
MJ Averner, ML Brock, JP Jost
The Journal of biological chemistry 1972
The Binding of Phytohemagglutinins to Human Platelet Plasma Membranes
PW Majerus, GN Brodie
The Journal of biological chemistry 1972
Insulin Action in Isolated Rat Thymocytes
ID Goldfine, P Sherline
The Journal of biological chemistry 1972
The roles of calcium and cyclic AMP in the stimulatory action of parathyroid hormone on thymic lymphocyte proliferation
JF Whitfield, JP MacManus, T Youdale, DJ Franks
Journal of Cellular Physiology 1971
Restoration of contact-inhibited growth to transformed cells by dibutyryl adenosine 3':5'-cyclic monophosphate
JR Sheppard
Proceedings of the National Academy of Sciences 1971
The effect of prostaglandins on cyclic AMP production and cell proliferation in thymic lymphocytes
DJ Franks, JP Macmanus, JF Whitfield
Biochemical and Biophysical Research Communications 1971
Immunopharmacology
BB Levine
Immunopharmacology 1968
Advances in Lipid Research
AM Scanu
Advances in Lipid Research 1965
Metabolic Inhibitors
QH Gibson
Metabolic Inhibitors 1963

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