Citations to this article

Hormone synthesis and secretion by rat parathyroid glands in tissue culture
William Y. W. Au, … , Paula H. Stern, Lawrence G. Raisz
William Y. W. Au, … , Paula H. Stern, Lawrence G. Raisz
Published September 1, 1970
Citation Information: J Clin Invest. 1970;49(9):1639-1646. https://doi.org/10.1172/JCI106381.
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Research Article

Hormone synthesis and secretion by rat parathyroid glands in tissue culture

Abstract

Rat parathyroid glands maintained in organ culture secrete biologically active parathyroid hormone (PTH) and synthesize and secrete labeled proteins from 3H- or 14C-labeled amino acids added to the medium. The amounts of biological activity and labeled protein in the medium are both inversely proportional to the calcium concentration. Some of the labeled low molecular weight protein was identified as PTH which had been synthesized and secreted in culture by preliminary isolation on Sephadex G-100 columns and further purification using an antibody to bovine PTH which cross-reacted with rat PTH. The cross-reacting antibody inhibited the biological effects of rat PTH and caused hypocalcemia in intact rats. The antibody bound some of the labeled low molecular weight protein of the medium at neutral pH so that it migrated as a large molecular weight complex on Sephadex. Biologically active, labeled PTH was recovered by dissociation of this complex in acid and rechromatography.

Authors

William Y. W. Au, Alan P. Poland, Paula H. Stern, Lawrence G. Raisz

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Year: 2020 2010 1997 1996 1984 1983 1982 1981 1979 1978 1977 1976 1975 1974 1972 1971 Total
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Citations to this article (25)

Title and authors Publication Year
Parathyroid Glands in Chronic Kidney Disease
A Covic, D Goldsmith, PA Torres 		2020
Alan Poland, MS, MD: 1940–2020 Poisons as Probes of Biological Function
MN Avilla, CA Bradfield, E Glover, ME Hahn, KM Malecki, PH Stern, RH Wilson 		Chemical Research in Toxicology 2020
Vitamins & Hormones
KJ Hare, FK Knop 		Vitamins & Hormones 2010
Osteoporosis
DL Glaser, FS Kaplan 		Spine 1997
Direct effect of phosphorus on PTH secretion from whole rat parathyroid glands in vitro
E Slatopolsky, J Finch, M Denda, C Ritter, M Zhong, A Dusso, PN MacDonald, AJ Brown 		Journal of Clinical Investigation 1996
Direct effect of phosphorus on PTH secretion from whole rat parathyroid glands in vitro
E Slatopolsky, J Finch, M Denda, C Ritter, M Zhong, A Dusso, PN MacDonald, AJ Brown 		Journal of Clinical Investigation 1996
Inhibition by 1,25 dihydroxycholecalciferol of hormonal secretion of rat parathyroid gland in organ culture
W Y.W.Au 		Calcified Tissue International 1984
Primary monolayer cell culture of bovine parathyroids: Effects of calcium, isoproterenol and growth factors
RR MacGregor, MP Sarras, A Houle, DV Cohn 		Molecular and Cellular Endocrinology 1983
Phosphorylation of parathyroid secretory protein
G Bhargava, J Russell, LM Sherwood 		Proceedings of the National Academy of Sciences 1983
Parathyroid function in persistent hyperparathyroidism: Relationship to gland size
DA McCarron, RS Muther, B Lenfesty, WM Bennett 		Kidney International 1982
Disorders of Mineral Metabolism
RK Rude, FR Singer 		Disorders of Mineral Metabolism 1982
Extraction, purification, and partial characterization of bovine parathyroid secretory protein
K Takatsuki, AB Schneider, KY Shin, LM Sherwood 		The Journal of biological chemistry 1981
Effects of calcium and cyclic nucleotides on rat calcitonin and parathyroid hormone secretion
WK Ramp, CW Cooper, AJ Ross, SA Wells 		Molecular and Cellular Endocrinology 1979
The secretion of parathormone and glycosylated proteins by parathyroid cells in culture
JJ Morrissey, JW Hamilton, DV Cohn 		Biochemical and Biophysical Research Communications 1978
I. Aspetti Anatomopatologici E Fisiopatologici Dell'Iperparatiroidismo Primario
D Fontana, A Tizzani, B Frea 		1977
Cortisol Stimulation of Parathyroid Hormone Secretion by Rat Parathyroid Glands in Organ Culture
WY Au 		Science 1976
Regulation of circulating parathyroid hormone levels: Normal physiology and consequences in disorders of mineral metabolism
JA Fischer, JW Blum, W Hunziker, U Binswanger 		Klinische Wochenschrift 1975
Biosynthesis of parathyroid hormone
TJ Martin 		Clinics in Endocrinology and Metabolism 1974
Bone-Resorbing Activity in Supernatants from Lymphoid Cell Lines
GR Mundy, RA Luben, LG Raisz, JJ Oppenheim, DN Buell 		New England Journal of Medicine 1974
Parathyroid Secretion: Discovery of a Major Calcium-Dependent Protein
B Kemper, JF Habener, A Rich, JT Potts 		Science 1974
Secretion of glucosamine-containing macromolecules by rat parathyroid glands in tissue culture
AA Licata, WY Au, LG Raisz 		Biochimica et Biophysica Acta (BBA) - General Subjects 1972
Endocrinology 1971
WB Blythe, D D'avila, HJ Guelman, LG Welt 		Endocrinology 1971 1972
Human parathyroid hormone: Glandular and secreted molecular species
CD Arnaud, GW Sizemore, SB Oldham, JA Fischer, HS Tsao, ET Littledike 		The American Journal of Medicine 1971
Dynamics of parathyroid hormone secretion in vitro
SB Oldham, JA Fischer, CC Capen, GW Sizemore, CD Arnaud 		The American Journal of Medicine 1971
Studies on the Biosynthesis in Vitro of Parathyroid Hormone
JW Hamilton, FW Spierto, RR MacGregor, DV Cohn 		The Journal of biological chemistry 1971

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