Citations to this article

Galactose-1-phosphate uridyltransferase and galactokinase activity in cultured human diploid fibroblasts and peripheral blood leukocytes: I. Analysis of transferase genotypes by the ratio of the activities of the two enzymes
Thomas A. Tedesco, William J. Mellman
Thomas A. Tedesco, William J. Mellman
Published December 1, 1969
Citation Information: J Clin Invest. 1969;48(12):2390-2397. https://doi.org/10.1172/JCI106205.
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Galactose-1-phosphate uridyltransferase and galactokinase activity in cultured human diploid fibroblasts and peripheral blood leukocytes: I. Analysis of transferase genotypes by the ratio of the activities of the two enzymes

Abstract

The specific activities of galactokinase and galactose-1-phosphate uridyltransferase were determined in peripheral blood leukocytes directly after separation from whole blood, and in cultured skin fibroblasts at various times during the subculture growth period. Growth curves were obtained for fibroblasts based on three different parameters: direct cell counts, total protein, and total deoxyribonucleic acid (DNA) content. At the time in culture when the specific activity of both enzymes was maximal and least variable, the ratio of transferase to galactokinase correlated well with the transferase genotypes of the original tissue donors. Leukocyte transferase: galactokinase ratios gave a similar distribution pattern.

Authors

Thomas A. Tedesco, William J. Mellman

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Year: 2008 1991 1988 1984 1983 1981 1979 1978 1977 1976 1975 1974 1973 1972 1971 1970 Total
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Citations to this article (37)

Title and authors Publication Year
Bovine viral diarrhea viruses differentially alter the expression of the protein kinases and related proteins affecting the development of infection and anti-viral mechanisms in bovine monocytes
GV Pinchuk, SR Lee, B Nanduri, KL Honsinger, JV Stokes, LM Pinchuk 		Biochimica et Biophysica Acta (BBA) - Proteins & Proteomics 2008
International Review of Cytology
RA Miller 		International Review of Cytology 1991
The Philadelphia variant of galactokinase in human erythrocytes: Physicochemical and catalytic properties
T Soni, M Brivet, N Moatti, A Lemonnier 		Clinica Chimica Acta 1988
Thymidine-kinase activity of cultured cells from individuals with inherited galactokinase deficiency
RC Schoen, SH Cox, RP Wagner 		The American Journal of Human Genetics 1984
Clinical Biochemistry
E Cooper, M Bowen 		Clinical Biochemistry 1984
Prenatal diagnosis of galactosemia and properties of galactose-1-phosphate uridyltransferase in erythrocytes of galactosemic variants as well as in human fetal and adult organs
YS Shin, W Endres, M Rieth, J Schaub 		Clinica Chimica Acta 1983
Influence of hexose conditions on glutamine oxidation of SV-40-transformed and diploid fibroblast human cell lines
M Kuchka, H B Markus, W J Mellman 		Biochemical Medicine 1981
Characteristics of galactokinase and galactose-1-phosphate uridyltransferase in cultivated fibroblasts and amniotic fluid cells
Y Shin-Buehring, H Leitner, H Henseleit, A Wirtz, B Haas, J Schaub 		Human Genetics 1979
Galactosemia: Alterations in Sulfate Metabolism Secondary to Galactose-1-Phosphate Uridyltransferase Deficiency
TA Tedesco, KL Miller 		Science 1979
Biochemical studies of a human low-activity galactose-1-phosphate uridyl transferase variant
WG Ng, F Kline, J Lin, R Koch, GN Donnell 		Journal of Inherited Metabolic Disease 1978
Enzymes of galactose metabolism in human hair roots
C Bruyn, C Haymakers, A Wensing, T L.oei, P Hosli 		British Journal of Dermatology 1977
The Philadelphia variant of galactokinase
TA Tedesco, KL Miller, BE Rawnsley, MC Adams, HB Markus, KG Orkwiszewski, WJ Mellman 		The American Journal of Human Genetics 1977
Growth, Nutrition, and Metabolism of Cells in Culture
HL Leffert, KS Koch 		Growth, Nutrition, and Metabolism of Cells in Culture 1977
Prenatal diagnosis of galactosemia
WG Ng, GN Donnella, WR Bergrena, O Alfia, MS Golbusa 		Clinica Chimica Acta 1977
The effect of pH on incorporation of galactose by a normal human cell line and cell lines from patients with defective galactose metabolism
HZ Hill 		Journal of Cellular Physiology 1976
Inhibitory action of D-galactose on phagocyte metabolism and function
WJ Litchfield, WW Wells 		Infection and immunity 1976
Galactose-1-phosphate uridyltransferase in cultured cells
AM Monk, JB Holton 		Clinica Chimica Acta 1976
Glk: A locus controlling galactokinase activity in the mouse
JD Mishkin, BA Taylor, WJ Mellman 		Biochemical Genetics 1976
Studies on the regulation of the three enzymes of the leloir pathway in cultured mammalian cells. I. Effect of substitution of galactose for glucose as the sole hexose in the medium in human diploid cell strains and in a rat hepatoma line
ES Stern, RS Krooth 		Journal of Cellular Physiology 1975
Studies on the regulation of the three enzymes of the leloir pathway in cultured mammalian cells II. A Search for quantitative interrelationships between the three enzymes activities
ES Stern, RS Krooth 		Journal of Cellular Physiology 1975
Galactose and glucose metabolism in galactokinase deficient, galactose-1-P-uridyl transferase deficient and normal human fibroblasts
TB Friedman, RJ Yarkin, CR Merril 		Journal of Cellular Physiology 1975
Human erythrocyte galactokinase and galactose-1-phosphate uridylyltransferase: a population survey
TA Tedesco, KL Miller, BE Rawnsley, MT Mennuti, RS Spielman, WJ Mellman 		The American Journal of Human Genetics 1975
Brain Mechanisms in Mental Retardation
TA Tedesco 		Brain Mechanisms in Mental Retardation 1975
Induction and analysis of gene mutations in cultured mammalian somatic cells
EH Chu 		Genetics 1974
Assignment of the human gene for hexose-1-phosphate uridylyltransferase to chromosome 3
TA Tedesco, R Diamond, KG Orkwiszewski, HJ Boedecker, CM Croce 		Proceedings of the National Academy of Sciences 1974
Tissue Culture
RS Krooth, EK Sell 		Tissue Culture 1973
Galactokinase deficiency: Clinical and biochemical findings in a new kindred
WR Pickering, RR Howell 		The Journal of Pediatrics 1972
Uridine diphosphoglucose pyrophosphorylase and uridine diphosphogalactose pyrophosphorylase in human skin fibroblasts derived from normal and galactosemic individuals
CM Chacko, L McCrone, HL Nadler 		Biochimica et Biophysica Acta (BBA) - Enzymology 1972
Normal pregnancy and childbirth in a galactosemic woman
T A Tedesco, G Morrow 3rd, W J Mellman 		The Journal of Pediatrics 1972
Human Galactose 1-Phosphate Uridyltransferase
TA Tedesco 		The Journal of biological chemistry 1972
Galactokinase: Evidence for a New Racial Polymorphism
TA Tedesco, R Bonow, K Miller, WJ Mellman 		Science 1972
Galactosemia: Evidence for a Structural Gene Mutation
TA Tedesco, WJ Mellman 		Science 1971
Purification and Properties of Galactokinase from Human Red Blood Cells
KG Blume, E Beutler 		The Journal of biological chemistry 1971
Evaluation of a heterozygote test for maple syrup urine disease in leucocytes and cultured fibroblasts
U Langenbeck, HW Rdiger, M Schulze-Schencking, W Keller, D Brackertz, HW Goedde 		Human Genetics 1971
The action of mendelian genes in human diploid cell strains
RS Krooth, EK Sell 		Journal of Cellular Physiology 1970
Interallelic complementation in hybrid cells derived from human diploid strains deficient in galactose-1-phosphate uridyl transferase activity
HL Nadler, CM Chacko, M Rachmeler 		Proceedings of the National Academy of Sciences 1970
Human Cell Cultures: Their Use in the Investigation and Diagnosis of Disease
WJ Mellman, G Kohn 		The Medical clinics of North America 1970

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