Citations to this article

Partial purification and properties of a plasminogen activator from human erythrocytes
M. Semar, … , L. Skoza, A. J. Johnson
M. Semar, … , L. Skoza, A. J. Johnson
Published October 1, 1969
Citation Information: J Clin Invest. 1969;48(10):1777-1785. https://doi.org/10.1172/JCI106144.
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Research Article

Partial purification and properties of a plasminogen activator from human erythrocytes

Abstract

The lysis time of euglobulin clots made with whole blood (plasma and red cells) was very much shorter than that of clots made with plasma alone, indicating a fibrinolytic component in red cells. A plasminogen activator was found in the stroma-free hemolysate, and proteolytic activity was found in the stromal fraction. The plasminogen activator, purified by using diethylaminoethyl-cellulose (DEAE-cellulose) in a batch procedure followed by column chromatography, was called erythrokinase (EK). On preliminary characterization, EK appears to activate human and bovine plasminogen in a manner similar to urokinase (UK), as determined by fibrinolytic and caseinolytic assays. The two enzymes can be separated by DEAE chromatography and acrylamide-gel electrophoresis, however, and they hydrolyze acetyl-L-lysine methyl ester and benzoyl arginine methyl ester at different rates.

Authors

M. Semar, L. Skoza, A. J. Johnson

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Total citations by year

Year: 2011 2001 1997 1984 1983 1981 1979 1976 1974 1973 1972 1971 Total
Citations: 1 1 1 1 3 2 1 1 6 2 4 1 24
Citation information

Citations to this article (24)

Title and authors Publication Year
Annual Reports in Medicinal Chemistry
JJ Liu, TW Lee 		Annual Reports in Medicinal Chemistry Volume 46 2011
Fibrinolytics and Antifibrinolytics
F Bachmann 		2001
Bovine erythrocyte haemolysates enhance plasminogen activation by tissue-type plasminogen activator
M Yamada, T Horiuchi, T Oribe, S Yamamoto, I Sugie, P A Gentry 		Veterinary Research Communications 1997
Isolation and characterization of a urokinase-type plasminogen activator (Mr = 54,000) from cultured human endothelial cells indistinguishable from urinary urokinase
FM Booyse, G Osikowicz, S Feder, J Scheinbuks 		The Journal of biological chemistry 1984
Identification of the plasminogen activator(s) produced by the transformed liver cell line, SK-HEP-1
GH Barlow, SL Firestone, KC Robbins 		Thrombosis Research 1983
The Physiology of Hemostasis
D Ogston 		1983
Purification and characterization of the biliary plasminogen activator bilokinase
S Oshiba, T Ariga 		The Journal of biological chemistry 1983
Plasminogen activator content of neoplastic and benign human prostate tissues; fibrin augmentation of an activator activity
SM Camiolo, G Markus, JL Evers, H Hobika, JL Depasquale, S Beckley, JP Grimaldi 		International Journal of Cancer 1981
Immunological identification and comparison of plasminogen activator forms in cultured normal human endothelial cells and smooth muscle cells
FM Booyse, J Scheinbuks, J Radek, G Osikowicz, S Feder, AJ Quarfoot 		Thrombosis Research 1981
Neutral proteases from human and ovine erythrocyte membranes
GK Scott, TB Kee 		International Journal of Biochemistry 1979
Relationship between cell surface protease activity and doubling time in various normal and transformed cells
VB Hatcher, MS Wertheim, CY Rhee, G Tsien, PG Burk 		Biochimica et Biophysica Acta (BBA) - General Subjects 1976
Increased protease levels in transformed cells: A casein overlay assay for the detection of plasminogen activator production
AR Goldberg 		Cell 1974
Some properties of the tissue plasminogen activator from the pig heart
A Hijikata, K Fujimoto, H Kitaguchi, S Okamoto 		Thrombosis Research 1974
Interaction between red cell membranes and fibrinogen
M Murray, DE Rearick 		Thrombosis Research 1974
The Red Blood Cell
GJ Brewer 		The Red Blood Cell 1974
Blood
WH Crosby 		Blood 1974
Proteinase Inhibitors
H Fritz, H Tschesche, LJ Greene, E Truscheit 		1974
The Influence of Haemoglobin on the Fibrinolytic Enzyme System
D Ogston, RJ Herbert 		Scandinavian Journal of Haematology 1973
Proteolytic cleavage by plasmin of the HA polypeptide of influenza virus: Host cell activation of serum plasminogen
SG Lazarowitz, AR Goldberg, PW Choppin 		Virology 1973
Increase in Fibrinogen and Fibrin-Related Antigen in Human Serum Due to In Vitro Lysis of Fibrin by Thrombin
C Merskey, AJ Johnson, P Lalezari 		Journal of Clinical Investigation 1972
Clinical experience with brinase
EP Frisch 		Journal of clinical pathology 1972
Disseminated intravascular coagulation (DIC): An approach
RW Colman, SJ Robboy, JD Minna 		The American Journal of Medicine 1972
The Physiology of Fibrinolysis
T Astrup, S Thorsen 		Medical Clinics of North America 1972
11. ON THE ORIGIN OF THE PROTEOLYTIC ACTIVITY RESPONSIBLE FOR THE RELEASE OF FIBRINOGEN-FIBRIN RELATED ANTIGEN IN VITRO*
AJ Johnson, C Merskey 		Scandinavian Journal of Haematology 1971

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