Protein starvation and the small intestine: III. Incorporation of orally and intraperitoneally administered l-leucine 4,5-³H into intestinal mucosal protein of protein-deprived rats

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Abstract

Weanling rats were fed diets which contained either no protein or 27% protein. In one experiment after 23-35 days both groups were given l-leucine-4,5-3H either intragastrically or intraperitoneally and then sacrificed 24 hr later. In a second experiment animals were given these diets for 21 days and sacrificed 3, 6, or 12 hr after either intragastric or intraperitoneal administration of the labeled leucine. In both experiments the intestinal mucosa of proximal and distal segments of the small intestine was scraped, weighed, the protein concentration measured, and the specific activty of the mucosal protein was determined.

Authors

Jan S. Hirschfield, Fred Kern Jr.