An inhibitory effect of furosemide on sodium reabsorption by the proximal tubule of the rat nephron

Barry M. Brenner; Robert I. Keimowitz; Fred S. Wright; Robert W. Berliner

doi:10.1172/JCI105985

An inhibitory effect of furosemide on sodium reabsorption by the proximal tubule of the rat nephron

Barry M. Brenner, Robert I. Keimowitz, Fred S. Wright, and Robert W. Berliner

Published February 1, 1969 - More info

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Abstract

The evidence from previous micropuncture studies for an inhibitory effect of furosemide on proximal sodium reabsorption in the rat has been conflicting. Intrinsic reabsorptive capacity, estimated in free flow and shrinking drop experiments, has been reported to be depressed, whereas fractional reabsorption usually remains unchanged.

We have recently reported that, during conditions of elevated intraluminal hydrostatic pressure, unless care is taken to prevent retrograde flow of tubule fluid from more distal sites, the concentration of inulin in late proximal fluid is often factitiously elevated. Since furosemide raises intraluminal pressures, often markedly, the failure to detect a depression of fractional reabsorption might be the consequence of retrograde contamination during fluid collection.

Experiments were designed to compare the effect of furosemide on fractional sodium reabsorption by the proximal tubule when collections were obtained with distal oil blocks of conventional length as well as with unusually long blocks of oil of low and high viscosities.

When reflux is prevented, fractional sodium reabsorption is usually depressed by furosemide, whereas when conventional distal blocks are used, the calculated values for fractional reabsorption either remain unchanged or increase. Simultaneous measurements of nephron glomerular filtration rate indicate that the latter is the consequence of retrograde contamination.