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Research Article Free access | 10.1172/JCI105850
Department of Medicine, Los Angeles County—University of Southern California Medical Center, Los Angeles, California 90033
Find articles by Bethune, J. in: JCI | PubMed | Google Scholar
Department of Medicine, Los Angeles County—University of Southern California Medical Center, Los Angeles, California 90033
Find articles by Turpin, R. in: JCI | PubMed | Google Scholar
Published July 1, 1968 - More info
A procedure for bioassaying parathyroid hormone-like activity in human urine has been developed. 24-hr urine samples were concentrated with dry Sephadex G-25 and bioassayed in the young thyroparathyrocauterized mouse by the measurement of whole blood calcium. Recovery of biological activity and radioiodinated beef parathyroid hormone was over 80%. Normal subjects usually excreted less than 30 U (USP) of activity per day while 18 patients with proven primary hyperparathyroidism excreted a mean of 182 U/day (USP). The activity was not found in 7 patients with hypoparathyroidism or in 5 patients with carcinoma of the breast, but was present in 9 patients with uremia and in 5 with carcinoma of the lung and hypercalcemia.