Partition of calcium, phosphate, and protein in the fluid phase aspirated at calcifying sites in epiphyseal cartilage

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Abstract

A reproducible method, adapted from renal micropuncture techniques, was developed for sampling 10-40 mμl of a clear fluid from epiphyseal cartilage of normal or rachitic rats in vivo, either from the hypertrophic cell zone (Cf1) or surface resting cell cartilage (Lf1).

Authors

David S. Howell, Julio C. Pita, Juan F. Marquez, Juan E. Madruga