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Citations to this article

In vitro synthesis of immunoglobulin by rheumatoid synovial membrane
J. Donald Smiley, … , Charlotte Sachs, Morris Ziff
J. Donald Smiley, … , Charlotte Sachs, Morris Ziff
Published March 1, 1968
Citation Information: J Clin Invest. 1968;47(3):624-632. https://doi.org/10.1172/JCI105758.
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In vitro synthesis of immunoglobulin by rheumatoid synovial membrane

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Abstract

A technique for the in vitro culture of rheumatoid synovial tissue with 14C-amino acids and isolation and quantitation of the newly synthesized immunoglobulins has been developed. This technique has been used to compare immunoglobulin synthesis of 12 rheumatoid synovia with that of synovia from nonarthritic patients and with that of normal human lymph nodes and spleen. In addition, the spleen of a patient with Felty's syndrome has also been examined. Immunoglobulin synthesis in rheumatoid synovia has been shown to be quantitatively and qualitatively similar to that of normal human spleen and lymph nodes although somewhat less active than the Felty's syndrome spleen examined. 79% of the immunoglobulin produced in rheumatoid synovia was of the IgG type, whereas IgM comprised 10% and IgA, 11% of the total. Less than 10% of the IgM synthesized was found to be rheumatoid factor. A fraction containing approximately 90% of its radioactivity in the form of IgG has been obtained for further studies.

Authors

J. Donald Smiley, Charlotte Sachs, Morris Ziff

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O Duke, GS Panayi, G Janossy, LW Poulter
Clinical & Experimental Immunology 1982
Evidence for the local production and utilization of immune reactants in rheumatoid arthritis
F Cecere, J Lessard, S Mcduffy, RM Pope
Arthritis & Rheumatism 1982
Quantitation of Cells Secreting Immunoglobulins after Elution from Rheumatoid Synovial Tissue
T Egeland, T Lea, OJ Mellbye, JA Pahle, T Ottesen, JB Natvig
Scandinavian Journal of Immunology 1982
Biochemical Immunological Pharmacology
PE Lipsky
Biochemical Immunological Pharmacology 1982
Natural killer cell activity of mononuclear cells from rheumatoid patients measured by a conjugate-binding cytotoxicity assay
E Reinitz, PA Neighbour, AI Grayzel
Arthritis & Rheumatism 1982
Apheresis therapy: spin-drying the blood
TJ Hamblin
BMJ : British Medical Journal 1982
Bone and Joint Disease
CL Berry
1982
Human mononuclear cell factors mediate cartilage matrix degradation through chondrocyte activation
HE Jasin, JT Dingle
Journal of Clinical Investigation 1981
Physiology and pathology of rheumatoid factors
DA Carson, JL Pasquali, CD Tsoukas, S Fong, SF Slovin, SK Lawrance, L Slaughter, JH Vaughan
Springer Seminars in Immunopathology 1981
Protein synthesis in rheumatoid synovial tissue
JD Smiley, WL Hoffman
Springer Seminars in Immunopathology 1981
RHEUMATOID ARTHRITIS: A DISEASE OF T-LYMPHOCYTE/MACROPHAGE IMMUNOREGULATION
G Janossy, O Duke, LW Poulter, G Panayi, M Bofill, G Goldstein
The Lancet 1981
Phagocytes and Substrates in the Joint
M Ziff
Scandinavian Journal of Rheumatology 1981
Immune response of the ileum to invasive Escherichia coli diarrheal disease in rabbits
PD O'Hanley, JR Cantey
Infection and immunity 1981
The Biochemistry of the Human Synovial Lining with Special Reference to Alterations in Metabolism in Rheumatoid Arthritis
B Henderson
Pathology - Research and Practice 1981
Fibronectin in synovial fluid and tissue in rheumatoid arthritis
T Vartio, A Vaheri, RV Essen, H Isomäki, S Stenman
European Journal of Clinical Investigation 1981
Inflammatory synovial tissue mononuclear cells release leucocyte migration inhibition factor in antigen- and mitogen-free cultures
T Egeland, OJ Mellbye, JA Pahle
Annals of the rheumatic diseases 1981
Immunological Aspects of Rheumatology
WC Dick
1981
Platelet-activating activity in synovial fluids of patients with rheumatoid arthritis, juvenile rheumatoid arthritis, gout, and noninflammatory arthropathies
C Shapleigh, FH Valone, PH Schur, EJ Goetzl, KF Austen
Arthritis & Rheumatism 1980
Observations on some properties of immune complexes in a rheumatoid arthritis patient presenting with simultaneous synovitis and pleurisy
JT Halla, RE Schrohenloher, JE Volanakis
Arthritis & Rheumatism 1980
Laboratory Findings in Patients with Psoriasis, with Special Reference to Immunological Parameters, Associations with Arthropathy and Sacro-Iliitis
O Vinje, P Møller, OJ Mellbye
Scandinavian Journal of Rheumatology 1980
Local immunoglobulin synthesis in periodontal disease
ET Lally, PC Baehni, WP McArthur
Journal of Periodontal Research 1980
Enhanced in vitro synthesis of igm rheumatoid factor in rheumatoid arthritis
WJ Koopman, RE Schrohenloher
Arthritis & Rheumatism 1980
A sensitive radioimmunoassay for quantitation of igm rheumatoid factor
WJ Koopman, RE Schrohenloher
Arthritis & Rheumatism 1980
Granulocyte-Specific Antinuclear Antibodies.: Possible Significance for the Pathogenesis, Clinical Features and Diagnosis of Rheumatoid Arthritis
A Wilk
Allergy 1980
Production of human antithyroglobulin in vitro
GN Beall, SR Kruger
Clinical Immunology and Immunopathology 1980
Soluble Immune Complexes in Human Disease
UE Nydegger, JS Davis
Critical Reviews in Clinical Laboratory Sciences 1980
Inflammation: Mechanisms and Treatment
DA Willoughby, JP Giroud
1980
Enhancement of lymphocyte response to PHA by lysosomal enzymes from polymorphonuclear leukocytes of RA joint fluid: I. Biological effect on T lymphocyte function
T Abe, C Morimoto, T Toguchi, M Kiyotaki, M Homma
Inflammation 1980
The nonspecific inhibitory effect of synovial tissue extracts on leucocyte migration in vitro
JE Morgan, ND Hall, AJ Collins, PA Bacon
Annals of the rheumatic diseases 1980
ANTITHYROID-ANTIBODY ACTIVITY IN THE SYNOVIAL FLUID OF PATIENTS WITH VARIOUS ARTHRITIDES
DR Blake, E Stansfield, AM Mcgregor, BR Smith
The Lancet 1979
Senile Cardiac Amyloidosis: Evidence of Two Different Amyloid Substances in the Ageing Heart
P Westermark, B Johansson, JB Natvig
Scandinavian Journal of Immunology 1979
Increased Antibody-Dependent Cell-Mediated Cytotoxicity by Monocytes from Patients with Rheumatoid Arthritis
PH Waytz, SD Douglas
Arthritis & Rheumatism 1979
T lymphocyte subpopulations in synovial fluid of patients with rheumatic disease
G Biberfeld, E Nilsson, P Biberfeld
Arthritis & Rheumatism 1979
Effect of gold sodium thiomalate on murine lymphocyte functions
JJ Jennings, S Macrae, RM Gorczynski
Clinical & Experimental Immunology 1979
Lymphocyte depletion by continuous flow cell centrifugation in rheumatoid arthritis
J Karsh, DG Wright, JH Klippel, JL Decker, AB Deisseroth, MW Flye
Arthritis & Rheumatism 1979
Immune Complexes and the Evolution of Lyme Arthritis: Dissemination and Localization of Abnormal C1q Binding Activity
JA Hardin, AC Steere, SE Malawista
New England Journal of Medicine 1979
The Antigens
PJ Lachmann
The Antigens 1979
Immunological studies in juvenile rheumatoid arthritis
R K Chandra, S Bhatnagar, O P Ghai
The Indian Journal of Pediatrics 1979
Immune complex detection and complement activity in rheumatoid arthritis: a comparative study of a radioimmunoassay using monoclonal rheumatoid factor, gel diffusion techniques and C4 activity
JT Halla, JE Volanakis, JG Hardin, RE Schrohenloher
Clinical & Experimental Immunology 1978
Lymphocyte migratory pathways in adjuvant disease. I. Distribution of 51Cr-labeled thoracic duct lymph-borne
RH Kelly, VS Harvey
The American Journal of Pathology 1978
Susceptibility of rheumatoid and nonrheumatoid synovial cells to antibody-dependent cell-mediated cytotoxicity
MM Griffiths, CB Smith, BJ Pepper
Arthritis & Rheumatism 1978
Antigen-induced arthritis. decreased proteoglycan content and inhibition of proteoglycan synthesis in articular cartilage
DA Lowther, JD Sandy, VB Santer, HL Brown
Arthritis & Rheumatism 1978
Synthesis by an Established Lymphocyte Cell Line From a Rheumatoid Synovium
BC Gilliland, DK Ford, M Mannik
Arthritis & Rheumatism 1978
Subclasses IgA1 and IgA2 in serum and synovial fluid in rheumatoid arthritis and reactive synovitis of local origin
Z Hrncir, M Tichy
Annals of the rheumatic diseases 1978
Electron Microscopy and Synovial Pathology
KD Muirden, K Rogers
Australian and New Zealand Journal of Medicine 1978
THE IMMUNOLOGICAL PATHOGENESIS OF RHEUMATOID ARTHRITIS
LE Glynn
The Medical Journal of Australia 1978
The Interaction of Immunocompetent Cells and Chronic Inflammation as Exemplified by Rheumatoid Arthritis
JM Dayer, SM Krane
Clinics in rheumatic diseases 1978
Inhibition of antigen- and mitogen-induced human lymphocyte proliferation by gold compounds
PE Lipsky, M Ziff
Journal of Clinical Investigation 1977
Cathepsin D Agglutinators and Neutral Protease Agglutinators in Rheumatoid Arthritis
K Fehr, G Artmann, M Velvart, A Bʼnni
Arthritis & Rheumatism 1977
Immunohistochemical localization of prostaglandin e in rheumatoid synovial tissues
G Husby, AD Bankhurst, RC Williams
Arthritis & Rheumatism 1977
Enhancement of in vitro immunoglobulin synthesis of rheumatoid lymphocytes by aggregated human gamma globulin
K Yamasaki, M Ziff
Arthritis & Rheumatism 1977
The biology of the rheumatoid synovial cell
DM Haselwood, JJ Castles
The Western journal of medicine 1977
Rheumatoid factor plaque-forming cells in rheumatoid synovial tissue
MM Taylor-Upsahl, TG Abrahamsen, JB Natvig
Clinical & Experimental Immunology 1977
Immunoglobulin and specific-antibody synthesis in vitro by enteral and nonenteral lymphoid tissues after subcutaneous cholera immunization
AM Svennerholm, J Holmgren
Infection and immunity 1977
Immunofluorescence studies for immunoglobulins and complement C3 in synovial joint membranes in psoriatic arthritis
O Fyrand, OJ Mellbye, JB Natvig
Clinical & Experimental Immunology 1977
Cathepsin D Agglutinators in Rheumatoid Arthritis
G Artmann, K Fehr, A Böni
Arthritis & Rheumatism 1977
Levels of immunoglobulin E (IgE) in paired examinations of serum and synovial fluid in rheumatoid arthritis and reactive synovitis of local origin
Z Hrnc̆ír̆, M Tichý, J S̆ims̆a, M Salavec, J Vavr̆ina
Clinica Chimica Acta 1977
Local synovial synthesis of oligoclonal measles virus antibodies and of smooth muscle antibodies in a case of atypical rheumatoid arthritis
B Vandvik, OJ Mellbye, E Norrby
Annals of the rheumatic diseases 1977
Perspectives in Inflammation: Future Trends and Developments
DA Willoughby, JP Giroud, GP Velo
1977
Mixed lymphocyte cultures in rheumatoid arthritis
P Stastny
Journal of Clinical Investigation 1976
Cytotoxic activity of rheumatoid and normal lymphocytes against allogeneic and autologous synovial cells in vitro
MM Griffiths, CB Smith, JR Ward, MR Klauber
Journal of Clinical Investigation 1976
Rheumatoid factor-producing cells detected by direct hemolytic plaque assay
JH Vaughan, T Chihara, TL Moore, DL Robbins, K Tanimoto, JS Johnson, R McMillan
Journal of Clinical Investigation 1976
Immunoglobulins IgG and IgM in synovial fluids of swine with erysipelothrix polyarthritis
JF Timoney, U Yarkoni
Veterinary Microbiology 1976
Predominance of t cells in the lymphocytic infiltrates of synovial tissues in rheumatoid arthritis
AD Bankhurst, G Husby, RC Williams
Arthritis & Rheumatism 1976
Selective inhibition of lymphocyte responsiveness to phytohaemagglutinin in patients with Reiter's syndrome
I Scher, A Ahmed, DM Strong, R Wistar
Clinical & Experimental Immunology 1976
Electron microscopic observations of immunoreactive cells in the rheumatoid synovial membrane
H Ishikawa, M Ziff
Arthritis & Rheumatism 1976
Complement and immunoglobulins in synovial fluid from synovectomized patients with rheumatoid arthritis
OJ Mellbye, B Naes, E Munthe
Annals of the rheumatic diseases 1976
Transformation of human lymphocytes in vitro by autologous and allogeneic rheumatoid synovial fluids
TD Kinsella
Annals of the rheumatic diseases 1976
EVIDENCE FOR AN INFECTIOUS ETIOLOGY OF RHEUMATOID ARTHRITIS
D Hamerman
Annals of the New York Academy of Sciences 1975
Synovial fluid lymphocytes in rheumatoid arthritis
PD Utsinger
Arthritis & Rheumatism 1975
THE ROLE OF THE LYMPHOCYTE AND ITS PRODUCTS IN THE PROPAGATION OF JOINT DISEASE
CM Pearson, HE Paulus, HI Machleder
Annals of the New York Academy of Sciences 1975
A Comparative Study of the Protein Pattern in Serum and Synovial Fluid
L Willumsen, J Friis
Scandinavian Journal of Rheumatology 1975
Immunologic characterization of the mononuclear cell infiltrates in rheumatoid synovia, in rheumatoid nodules, and in lip biopsies from patients with sjö;ugren's syndrome
H Tannenbaum, GS Pinkus, LG Anderson, PH Schur
Arthritis & Rheumatism 1975
Elution and Characterization of Lymphocytes from Rheumatoid Inflammatory Tissue
TG Abrahamsen, SS Froland, JB Natvig, J Pahle
Scandinavian Journal of Immunology 1975
Surface characteristics of synovial fluid and peripheral blood lymphocytes in inflammatory arthritis
AI Brenner, MA Scheinberg, ES Cathcart
Arthritis & Rheumatism 1975
LYMPHOKINES IN RHEUMATOID SYNOVITIS
P Stastny, M Rosenthal, M Andreis, D Cooke, M Ziff
Annals of the New York Academy of Sciences 1975
Presence of antibodies specific to cartilage-type collagen in rheumatoid synovial tissue
J Mestecky, EJ Miller
Clinical & Experimental Immunology 1975
Mechanism of trapping of immune complexes in joint collagenous tissues
HE Jasin
Clinical & Experimental Immunology 1975
Retardation of colony growth of in vitro bone marrow culture using sera from patients with felty' syndrome, disseminated lupus erythematosus (sle), rheumatoid arthritis, and other disease states
DJ Duckham, RL Rhyne, FE Smith, RC Williams
Arthritis & Rheumatism 1975
Identification of immunoglobulins and complement in rheumatoid articular collagenous tissues
TD Cooke, ER Hurd, HE Jasin, J Bienenstock, M Ziff
Arthritis & Rheumatism 1975
Lymphokines in the rheumatoid joint
P Stastny, M Rosenthal, M Andreis, M Ziff
Arthritis & Rheumatism 1975
Study of lymphoid cells from inflamed synovial membranes
G Loewi, EM Lance, J Reynolds
Annals of the rheumatic diseases 1975
T and B Lymphocytes in Peripheral Blood and Tissue Lesions in Sjögren's Syndrome
N Talal, RA Sylvester, TE Daniels, JS Greenspan, RC Williams
Journal of Clinical Investigation 1974
Antibacterial mechanisms of the lower respiratory tract. I. Immunoglobulin synthesis and secretion
WL Hand, JR Cantey
Journal of Clinical Investigation 1974
The Roles of IgG, IgM Rheumatoid Factor, and their Complexes in the Induction of Polymorphonuclear Leukocyte Chemotactic Factor from Complement
T Wagner, G Abraham, J Baum
Journal of Clinical Investigation 1974
RUBELLA-ANTIBODY TITRES IN RHEUMATOID ARTHRITIS
F Stanford
The Lancet 1974
MICROBIOLOGICAL HAZARDS OF INTRAVENOUS INFUSIONS
N Hirschhorn
The Lancet 1974
Comparative Investigation of Protein Concentration in Serum and Synovial Fluid
EM Veys
Scandinavian Journal of Rheumatology 1974
The biological activity of bovine cartilage preparations
JF Prudden, LL Balassa
Seminars in Arthritis and Rheumatism 1974
Cellular immunological aspects of rheumatoid arthritis
DT Yu, JB Peter
Seminars in Arthritis and Rheumatism 1974
In vitro studies on peripheral blood leucocytes in rheumatoid arthritis
HA Ménard, T Haim, AP Peltier
Annals of the rheumatic diseases 1974
B- and T-lymphocytes in rheumatoid synovitis and the effect of cyclophosphamide
M Ziff, ER Hurd, P Stastny
Proceedings of the Royal Society of Medicine 1974
Immunoglobulin E (IgE) levels in serum and synovial fluid in rheumatoid arthritis
GG Hunder, GJ Gleich
Arthritis & Rheumatism 1974
Rheumatoid Arthritis: Biosynthesis of Complement Proteins by Synovial Tissues
S Ruddy, HR Colten
New England Journal of Medicine 1974
Experimental arthritis produced by injection of mediators of delayed hypersensitivity
M Andreis, P Stastny, M Ziff
Arthritis & Rheumatism 1974
Relation of cellular infiltration of rheumatoid synovial membrane to its immune response
M Ziff
Arthritis & Rheumatism 1974
Virus resistance transferred from human rheumatoid cells to rabbit synovial cells. I. Methods and results of intra-articular injection of human cells into rabbit joints
C Smith, D Hamerman, R Janis, E Habermann
Annals of the rheumatic diseases 1974
Mycoplasmas and rheumatoid arthritis
SM Stewart, JJ Duthie, JM Mackay, BP Marmion, WR Alexander
Annals of the rheumatic diseases 1974
Die Behandlung der Rheumatoiden Arthritis mit D-Penicillamin
VR Ott
1974
Erworbene Herzfehler Entzündlicher Rheumatismus
O Hammer
1974
Immunologically induced acute synovitis in rabbits studies of immune complexes in synovial fluid
JR Hollister, GC Liang, M Mannik
Arthritis & Rheumatism 1973
Identification of Three Different Human Lymphocyte Populations by Surface Markers
SS Froland, JB Natvig
Immunological Reviews 1973
Immunological Characterization of Lymphocytes in Synovial Fluid from Patients with Rheumatoid Arthritis
SS FRphiLAND, JB Natvig, G Husby
Scandinavian Journal of Immunology 1973
Production of a macrophage migration inhibitory factor in rabbits with experimental arthritis
P Stastny, TD Cooke, M Ziff
Clinical & Experimental Immunology 1973
The Effect of IgG in Vitro on Leukocytes from Patients with Rheumatoid Arthritis
SS Froland, PI Gaarder
Scandinavian Journal of Immunology 1973
Experimental Arthritis, Light and Electron Microscope Studies of Antigen and Antibody Using Horseradish Peroxidase as Antigen
A Doble, J Dorling, LE Glynn, JA Webb, JH Wilcox
British Journal of Experimental Pathology 1973
Electron Microscopic Studies of Lymphoid Cells in the Rheumatoid Synovial Membrane
I Kobayashi, M Ziff
Arthritis & Rheumatism 1973
The pathogenesis of chronic inflammation in experimental antigen-induced arthritis. I. The role of antigen on the local immune response
TD Cooke, HE Jasin
Arthritis & Rheumatism 1972
Immunglobulin classes, subclasses and complexes of IgG rheumatoid factor in rheumatoid plasma cells
E Munthe, JB Natvig
Clinical & Experimental Immunology 1972
Chronic synovitis in rheumatoid arthritis
FR Schmid, JT Tesar
Journal of Chronic Diseases 1972
Salivary gland immunoglobulin and rheumatoid factor synthesis in Sjögren's syndrome
LG Anderson, NA Cummings, R Asofsky, MB Hylton, TM Tarpley, TB Tomasi, RO Wolf, GL Schall, N Talal
The American Journal of Medicine 1972
Immunoglobulins A, G, and M in synovial fluid in rheumatoid arthritis. Reactive synovitis of local origin and in post mortem synovial fluid
Z Hrncir, M Tichy, M Salavec, J Vavrina
Annals of the rheumatic diseases 1972
Serum Factor protecting against Experimental Arthritis
RJ Perper, AL Oronsky
Nature: New biology 1972
Response of the rheumatoid synovial membrane to exogenous immunization
JH Herman, J Bradley, M Ziff, JD Smiley
Journal of Clinical Investigation 1971
Permeability of human synovial membrane to plasma proteins. Relationship to molecular size and inflammation
I Kushner, JA Somerville
Arthritis & Rheumatism 1971
Macroglobulins in rheumatoid arthritis and allied diseases
N Svartz
Seminars in Arthritis and Rheumatism 1971
Characterization of IgG complexes in eluates from rheumatoid tissue
E Munthe, JB Natvig
Clinical & Experimental Immunology 1971
Studies of cryoprecipitates from synovial fluid of rheumatoid patients
A Cracchiolo, LS Goldberg, EV Barnett, R Bluestone
Immunology 1971
Progress in Immunology
IH Lepow
Progress in Immunology 1971
Human Anti-Human Gammaglobulins
E Munthe, JB Natvig
Human Anti-Human Gammaglobulins 1971
Rheumatic diseases of the aged: diagnosis and management
J S Paolino
Journal of the American Geriatrics Society 1971
Immunological reactivity to Mycoplasma fermentans in patients with rheumatoid arthritis. Preliminary communication.
Williams MH, Bruckner FE
Annals of the rheumatic diseases 1971
Further speculation on the pathogenesis of joint inflammation in rheumatoid arthritis
NJ Zvaifler
Arthritis & Rheumatism 1970
Agammaglobulinemia with polyarthritis and subcutaneous nodules
EV Barnett, A Winkelstein, HJ Weinberger
The American Journal of Medicine 1970
POSSIBLE ROLE OF MYCOPLASMA FERMENTANS IN PATHOGENESIS OF RHEUMATOID ARTHRITIS
MH Williams, J Brostoff, IM Roitt
The Lancet 1970
Immunological studies in tropical splenomegaly syndrome
JV Wells
Transactions of the Royal Society of Tropical Medicine and Hygiene 1970
On the Presence of Rheumatoid Factor in Joints before and after Synovectomy
I Goldie
Acta Rheumatologica Scandinavica 1970
Immunoglobulin synthesis in vitro by human thymus: comparison of myasthenia gravis and normal thymus
JD Smiley, J Bradley, D Daly, M Ziff
Clinical & Experimental Immunology 1969
Local Immune Response in the Kidneys of Rabbits with Nephrotoxic Serum Nephritis*
JD Lehmann, JW Smith, TE Miller, JA Barnett, JP Sanford
British Journal of Experimental Pathology 1969
Exchange of IgM and albumin between plasma and synovial fluid in rheumatoid arthritis
DL Brown, AG Cooper, R Bluestone
Annals of the rheumatic diseases 1969
Local immune response in experimental pyelonephritis
JD Lehmann, JW Smith, TE Miller, JA Barnett, JP Sanford
Journal of Clinical Investigation 1968
Serum immunoconglutinin levels in human amyloidosis and myelomatosis
TJ Muckle
Annals of the rheumatic diseases 1968
Free immunoglobulin light chains in connective tissue diseases
A Cooper, R Bluestone
Annals of the rheumatic diseases 1968
Autoimmunity in Rheumatoid Arthritis
RB Goudie, WW Buchanan
Scottish Medical Journal 1967

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