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Research Article Free access | 10.1172/JCI105652

Diffusion Equilibrium for Ammonia in the Kidney of the Acidotic Dog

William J. Stone, Sulamita Balagura, and Robert F. Pitts

The Department of Physiology, Cornell University Medical College, New York

‡

Fellow of the New York Heart Association. Present address: William Beaumont Hospital, El Paso, Tex.

§

Established Investigator of the American Heart Association. Address requests for reprints to Dr. Sulamita Balagura, Department of Physiology, Cornell University Medical College, 1300 York Avenue, New York 10021.

*

Received for publication 15 May 1967 and in revised form 22 June 1967.

This study was supported by U. S. Public Health Service grants AM 10101-02 and HE 00814.

Find articles by Stone, W. in: PubMed | Google Scholar

The Department of Physiology, Cornell University Medical College, New York

‡

Fellow of the New York Heart Association. Present address: William Beaumont Hospital, El Paso, Tex.

§

Established Investigator of the American Heart Association. Address requests for reprints to Dr. Sulamita Balagura, Department of Physiology, Cornell University Medical College, 1300 York Avenue, New York 10021.

*

Received for publication 15 May 1967 and in revised form 22 June 1967.

This study was supported by U. S. Public Health Service grants AM 10101-02 and HE 00814.

Find articles by Balagura, S. in: PubMed | Google Scholar

The Department of Physiology, Cornell University Medical College, New York

‡

Fellow of the New York Heart Association. Present address: William Beaumont Hospital, El Paso, Tex.

§

Established Investigator of the American Heart Association. Address requests for reprints to Dr. Sulamita Balagura, Department of Physiology, Cornell University Medical College, 1300 York Avenue, New York 10021.

*

Received for publication 15 May 1967 and in revised form 22 June 1967.

This study was supported by U. S. Public Health Service grants AM 10101-02 and HE 00814.

Find articles by Pitts, R. in: PubMed | Google Scholar

Published October 1, 1967 - More info

Published in Volume 46, Issue 10 on October 1, 1967
J Clin Invest. 1967;46(10):1603–1608. https://doi.org/10.1172/JCI105652.
© 1967 The American Society for Clinical Investigation
Published October 1, 1967 - Version history
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Abstract

Inflow of preformed ammonia in arterial blood, renal production of ammonia, outflow of ammonia in renal venous blood, and urinary excretion of ammonia were measured during the infusion of 15NH4Cl into one renal artery of dogs with chronic metabolic acidosis. Our results show that the specific activity of ammonia measured in the urine and that calculated in the renal pool agree within 95%. Pool specific activity is obtained by dividing the rate of infusion of isotope by the pool turnover rate, i.e., the sum of the rate of ammonia output in the urine and that in renal venous blood. An average of 35% of urinary ammonia is derived from arterial ammonia in these experiments.

We conclude that ammonia is distributed evenly throughout all phases of the kidney within a period less than the transit time of blood through the kidney. Furthermore, from the proportion of urinary ammonia we found to be derived from preformed arterial ammonia (35%), and from our previous demonstration that 73% of urinary ammonia derives from deamidation and/or deamination of plasma glutamine, alanine, glycine, and glutamate, we can account for all of the ammonia that leaves the kidney in renal venous blood and in urine.

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