Measurement of Intracellular pH of Skeletal Muscle with pH-sensitive Glass Microelectrodes

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Abstract

We used three methods to examine the relationship among intracellular pH, transmembrane potential, and extracellular pH. Single-barreled electrodes permitted the determination of resting potential and intracellular pH with a minimum of cellular injury. Double-barreled electrodes, which incorporated a reference as well as a pH-sensitive electrode in a single tip, facilitated the direct measurement of intracellular pH without the interposition of the transmembrane potential. Triple-barreled electrodes permitted measurement of intracellular pH during the controlled hyperpolarization or depolarization of the cell membrane.

Authors

Norman W. Carter, Floyd C. Rector Jr., David S. Campion, Donald W. Seldin