The Anticoagulant Activity of Lysosomal Cationic Proteins from Polymorphonuclear Leukocytes

Hussain I. Saba; Harold R. Roberts; John C. Herion

doi:10.1172/JCI105559

^{Department of Medicine, University of North Carolina School of Medicine, Chapel Hill, N. C.}

^{Department of Pathology, University of North Carolina School of Medicine, Chapel Hill, N. C.}

†

U. S. Public Health Service fellow in hematology.

‡

Address requests for reprints to Dr. John C. Herion, Dept. of Medicine, University of North Carolina School of Medicine, Chapel Hill, N. C. 27514.

Submitted for publication July 7, 1966; accepted December 22, 1966.

Supported in part by U. S. Public Health Service grants AI 04925, HE 06350, and TI AM 5345.

Find articles by Saba, H. in: JCI | PubMed | Google Scholar

^{Department of Medicine, University of North Carolina School of Medicine, Chapel Hill, N. C.}

^{Department of Pathology, University of North Carolina School of Medicine, Chapel Hill, N. C.}

†

U. S. Public Health Service fellow in hematology.

‡

Address requests for reprints to Dr. John C. Herion, Dept. of Medicine, University of North Carolina School of Medicine, Chapel Hill, N. C. 27514.

Submitted for publication July 7, 1966; accepted December 22, 1966.

Supported in part by U. S. Public Health Service grants AI 04925, HE 06350, and TI AM 5345.

Find articles by Roberts, H. in: JCI | PubMed | Google Scholar

^{Department of Medicine, University of North Carolina School of Medicine, Chapel Hill, N. C.}

^{Department of Pathology, University of North Carolina School of Medicine, Chapel Hill, N. C.}

†

U. S. Public Health Service fellow in hematology.

‡

Address requests for reprints to Dr. John C. Herion, Dept. of Medicine, University of North Carolina School of Medicine, Chapel Hill, N. C. 27514.

Submitted for publication July 7, 1966; accepted December 22, 1966.

Supported in part by U. S. Public Health Service grants AI 04925, HE 06350, and TI AM 5345.

Find articles by Herion, J. in: JCI | PubMed | Google Scholar

Published in Volume 46, Issue 4 on April 1, 1967
J Clin Invest. 1967;46(4):580–589. https://doi.org/10.1172/JCI105559.
© 1967 The American Society for Clinical Investigation

Published April 1, 1967 - Version history

A cationic protein fraction from rabbit polymorphonuclear leukocyte lysosomes has been shown to exert a potent anticoagulant effect on human blood in vitro. The anticoagulant activity is detectable in the whole blood clotting time, the recalcification time of platelet-rich plasma, the prothrombin time, the partial thromboplastin time, and the thromboplastin generation test. The lysosomal cationic proteins do not inhibit any of the known specific procoagulants. They appear to inhibit clotting by blocking the formation of intrinsic thromboplastin possibly by interfering with the role of phospholipids in the reaction involving Factors V and X and calcium.