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Citations to this article

Chloroquine stimulates nitric oxide synthesis in murine, porcine, and human endothelial cells.
D Ghigo, … , G Pescarmona, A Bosia
D Ghigo, … , G Pescarmona, A Bosia
Published August 1, 1998
Citation Information: J Clin Invest. 1998;102(3):595-605. https://doi.org/10.1172/JCI1052.
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Research Article Article has an altmetric score of 5

Chloroquine stimulates nitric oxide synthesis in murine, porcine, and human endothelial cells.

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Abstract

Nitric oxide (NO) is a free radical involved in the regulation of many cell functions and in the expression of several diseases. We have found that the antimalarial and antiinflammatory drug, chloroquine, is able to stimulate NO synthase (NOS) activity in murine, porcine, and human endothelial cells in vitro: the increase of enzyme activity is dependent on a de novo synthesis of some regulatory protein, as it is inhibited by cycloheximide but is not accompanied by an increased expression of inducible or constitutive NOS isoforms. Increased NO synthesis is, at least partly, responsible for chloroquine-induced inhibition of cell proliferation: indeed, NOS inhibitors revert the drug-evoked blockage of mitogenesis and ornithine decarboxylase activity in murine and porcine endothelial cells. The NOS-activating effect of chloroquine is dependent on its weak base properties, as it is exerted also by ammonium chloride, another lysosomotropic agent. Both compounds activate NOS by limiting the availability of iron: their stimulating effects on NO synthesis and inhibiting action on cell proliferation are reverted by iron supplementation with ferric nitrilotriacetate, and are mimicked by incubation with desferrioxamine. Our results suggest that NO synthesis can be stimulated in endothelial cells by chloroquine via an impairment of iron metabolism.

Authors

D Ghigo, E Aldieri, R Todde, C Costamagna, G Garbarino, G Pescarmona, A Bosia

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Total citations by year

Year: 2024 2023 2022 2021 2020 2019 2018 2017 2016 2015 2014 2013 2012 2011 2010 2009 2008 2007 2006 2005 2004 2003 2002 2001 2000 1999 Total
Citations: 1 1 3 2 2 4 4 2 2 4 8 4 8 1 5 1 3 5 5 3 1 4 4 1 4 2 84
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Citations to this article in year 2012 (8)

Title and authors Publication Year
Hematite Nanoparticles Larger than 90 nm Show No Sign of Toxicity in Terms of Lactate Dehydrogenase Release, Nitric Oxide Generation, Apoptosis, and Comet Assay in Murine Alveolar Macrophages and Human Lung Epithelial Cells
FS Freyria, B Bonelli, M Tomatis, M Ghiazza, E Gazzano, D Ghigo, E Garrone, B Fubini
Chemical Research in Toxicology 2012
The surface reactivity and implied toxicity of ash produced from sugarcane burning
JS le Blond, M Tomatis, CJ Horwell, C Dunster, F Murphy, I Corazzari, F Grendene, F Turci, E Gazzano, D Ghigo, BJ Williamson, C Oppenheimer, B Fubini
Environmental Toxicology 2012
Surface reactivity and cell responses to chrysotile asbestos nano-fibers
F Turci, M Colonna, M Tomatis, S Mantegna, G Cravotto, G Gulino, E Aldieri, D Ghigo, B Fubini
Chemical Research in Toxicology 2012
Physicochemical Determinants in the Cellular Responses to Nanostructured Amorphous Silicas
E Gazzano, M Ghiazza, M Polimeni, V Bolis, I Fenoglio, A Attanasio, G Mazzucco, B Fubini, D Ghigo
Toxicological Sciences 2012
Carbon in Intimate Contact with Quartz Reduces the Biological Activity of Crystalline Silica Dusts
M Ghiazza, M Tomatis, S Doublier, F Grendene, E Gazzano, D Ghigo, B Fubini
Chemical Research in Toxicology 2012
A case of urinary incontinence by hydroxychloroquine in a geriatric patient
C Carnovale, V Perrone, C Borsadoli, A Mambrini, A Speziali, G Froldi, S Antoniazzi, L Magistro, E Clementi, S Radice
Journal of Clinical Pharmacy and Therapeutics 2012
Chronic use of chloroquine disrupts the urine concentration mechanism by lowering cAMP levels in the inner medulla
TN von Bergen, MA Blount
American journal of physiology. Renal physiology 2012
Chronic use of chloroquine disrupts the urine concentration mechanism by lowering cAMP levels in the inner medulla
TN von Bergen, MA Blount
American journal of physiology. Renal physiology 2012

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