Altered placental development and intrauterine growth restriction in IGF binding protein-1 transgenic mice
Paul A. Crossey, … , Claire C. Pillai, John P. Miell
Paul A. Crossey, … , Claire C. Pillai, John P. Miell
Published August 1, 2002
Citation Information: J Clin Invest. 2002;110(3):411-418. https://doi.org/10.1172/JCI10077.
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Article Genetics

Altered placental development and intrauterine growth restriction in IGF binding protein-1 transgenic mice

Abstract

Research Article

Authors

Paul A. Crossey, Claire C. Pillai, John P. Miell

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Figure 1

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hIGFBP-1 gene expression in maternal decidua and fetal liver. (a) hIGFBP...
hIGFBP-1 gene expression in maternal decidua and fetal liver. (a) hIGFBP-1 mRNA in decidua of transgenic dams was detected by RT-PCR using a primer pair that specifically amplifies a 372-bp product from hIGFBP-1 cDNA. Lane 1, no template (negative control); lane 2, transgenic mouse liver RNA (positive control); lane 3, wild-type mouse decidua RNA (negative control); lanes 4–6, total RNA extracted decidua of transgenic females sacrificed at the indicated timepoints. M, 100-bp ladder. (b) hIGFBP-1 expression in fetal liver was detected as described in a. Lane 1, no template (negative control); lane 2, transgenic adult mouse liver RNA (positive control); lane 3, wild-type adult mouse liver RNA (negative control); lane 4, wild-type fetal liver RNA; lanes 5 and 6, liver RNA from transgenic fetuses at e14.5 and e17.5, respectively. M, molecular size markers.