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Regulation of interleukin 3 gene induction in normal human T cells.
S C Guba, … , C B Thompson, S G Emerson
S C Guba, … , C B Thompson, S G Emerson
Published December 1, 1989
Citation Information: J Clin Invest. 1989;84(6):1701-1706. https://doi.org/10.1172/JCI114352.
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Regulation of interleukin 3 gene induction in normal human T cells.

Abstract

The regulation of IL-3 gene induction in human peripheral blood T cells was studied. IL-3 gene expression was inducible by crosslinking of the T cell receptor/CD3 complex using anti-CD3 MAb G19-4. Anti-CD3-induced IL-3 gene expression was found to be limited to the CD28+ T cell subset and could be augmented by costimulating T lymphocytes with antibodies directed against CD28. IL-3 expression could also be induced by costimulation of T cells with both phorbol ester and ionomycin, which are thought to mimic the intracellular effects of T cell receptor-antigen interaction. However, unlike other lymphokines such as IL-2 or granulocyte-macrophage colony-stimulating factor, IL-3 gene expression is not induced by stimulation of cells with phorbol myristate acetate and anti-CD28. We conclude that IL-3 gene regulation is under stringent control since IL-3 gene expression occurs only in the CD28+ subset of T cells, and since IL-3 induction obligately requires increased intracellular calcium.

Authors

S C Guba, G Stella, L A Turka, C H June, C B Thompson, S G Emerson

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