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Aldosterone Stimulation of Riboflavin Incorporation into Rat Renal Flavin Coenzymes and the Effect of Inhibition by Riboflavin Analogues on Sodium Reabsorption
Daniel Trachewsky
Daniel Trachewsky
Published December 1, 1978
Citation Information: J Clin Invest. 1978;62(6):1325-1333. https://doi.org/10.1172/JCI109253.
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Research Article

Aldosterone Stimulation of Riboflavin Incorporation into Rat Renal Flavin Coenzymes and the Effect of Inhibition by Riboflavin Analogues on Sodium Reabsorption

Abstract

This study was designed to investigate a possible relationship between the effect of aldosterone upon urinary electrolytes and the incorporation of [14C]riboflavin into renal [14C]flavin mononucleotide (FMN) and [14C]flavin adenine dinucleotide (FAD). Adrenalectomized Sprague-Dawley rats that weighed between 185 and 210 g were pretreated with 15 μg/100 g body wt dexamethasone intraperitoneally. 16 h later they were administered aldosterone (1.5 μg/100 g body wt) and [14C]riboflavin (5.0 μCi/200 g body wt). The urethra of each rat was ligated, and the rats were sacrificed by decapitation 3 h later. The urine was aspirated from the bladders of each rat and analyzed for total Na+ and K+ excretion while the kidneys were removed and the formation of [14C]FMN and [14C]FAD was determined for each kidney. There was a significant increase in the formation of renal [14C]FMN and [14C]FAD (27.3 and 14.4%, respectively) after aldosterone treatment. Aldosterone significantly decreased the excretion of Na+ by 50%, and increased that of K+ by 55%.

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Daniel Trachewsky

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