The importance of antigen site density has been studied by means of a model passive hemagglutination system using human red cells coupled with sulfanilic acid groups. Relative site numbers were estimated from the covalent linkage of sulfanilic acid-35S to red cell membrane protein and the effective antigen site number was determined with 125I-labeled rabbit IgG antisulfanilic acid.
Leon W. Hoyer, Norma C. Trabold
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